RESUMO
The changes in synaptosomal membranes in comparison with those of macrophages and mitochondria during repeated psychogenic stress were evaluated using a specially designed multiprobe procedure. Activity of Na,K-ATPase activity was measured as a functional marker for synaptosomal membranes. The interaction of peritoneal macrophages with nitrobluetetrazole (NBT-test) was also evaluated. Some bioenergetic parameters were measured in mitochondria using spectrophotometric techniques. The data revealed profound structural and functional changes in synaptosomal membranes on 15th day of stress. Those in macrophage membranes, present on 3rd and 6th day were not accompanied by functional ones. In conclusion the present data suggest that stress repeated for 15 days leads to a modelled psychopathology in rats and induce selective structural and functional changes in synaptosomal membranes from the cerebral cortex. This model may be used for investigations of the effects of psychotropic drugs.
Assuntos
Córtex Cerebral/patologia , Membranas Intracelulares/análise , Estresse Psicológico/patologia , Sinaptossomos/análise , Animais , Córtex Cerebral/análise , Metabolismo Energético , Membranas Intracelulares/ultraestrutura , Macrófagos/análise , Masculino , Fluidez de Membrana , Mitocôndrias/análise , Ratos , ATPase Trocadora de Sódio-Potássio/análiseRESUMO
High levels of bradykinin (60--80 ng/ml) were found in abdominal transudate from a patient with nephrotic syndrome caused by chronic glomerulonephritis. The abdominal transudate contained neutral kininogenase and its precursor, identified with plasma kallikrein and prekallikrein, respectively, as well as both forms of kininogen, the low-molecular-weight form predominating. The abdominal transudate was characterized also by very low kininase activity and low levels of alpha 1-antitrypsin (0.46 g/l) and alpha 2-macroglobulin. Large amounts of very low density lipoproteins were present in the transudate. Despite the difference in total protein content between the abdominal transudate and the patient's serum (4.3 g/l and 48 g/l, respectively) their protein fraction composition was similar. The data obtained suggest that bradykinin is important in maintenance of long-lasting blood vessel hyperpermeability, which, in turn, is a driving force in the pathogenesis of refractory nephrotic edema.