Nanoencapsulation of apocynin and vanillic acid extracted from Picrorhiza kurroa Royle ex Benth plant roots and its characterisation.

Picrorhiza kurroa Royle ex Benth (P.kurroa) is an important medicinal plant in the ayurvedic system for treating various liver and inflammatory conditions. The present study aimed to extract the phytocompounds from various extracts (Acetone, Chloroform, Ethanol, Ethyl acetate, Hexane, and Methanol) of P. kurroa. Further, the major phytocompounds were nano-encapsulated by PLGA (Poly-lactic-co-glycolic acid) method and characterized to enhance activity towards the target. The highest polyphenolic value was found to be 323.2 ± 16.6 and 316.3 ± 12.1 µg GAEq./mg in ethanolic and methanolic extracts. The highest flavonoid value was found to be 280.3 ± 19.8 and 300.8 ± 15.2 in ethanolic and methanolic extracts µg QEq./mg. P. kurroa exhibited DPPH radical scavenging with IC50 of 38.2 ± 1.1 and 43.7 ± 1.8 µg/mL and also showed potent ferric reducing power and total antioxidant activities. The major phytocompounds, such as apocynin (AP) and vanillic acid (VA), were confirmed using HPLC. Further, the nano-encapsulation of apocynin and vanillic acid successfully achieved by PLGA methods. The average particle size of nano-encapsulated apocynin, vanillic acid is 350 nm, 204.4 nm, and zeta potential were -25.3 mv and -11.2 mv. Nanoformulations showed an apocynin and vanillic acid encapsulation efficiency of 93.6% and 93.3%, respectively. SEM and AFM confirmed the round and smooth morphology of the nanoparticles. The results of XRD confirmed the amorphous nature of nanoformulations. FTIR technique confirm the presence of biomolecules inside the polymer. The thermal stability of nanoformulations determined by DSC analysis showed endothermic peak. The prepared and characterization apocynin, vanillic acid nanoparticles revealed their good quality index, suggesting that potential use in pharmacy and phytotherapy as a source of natural antioxidant.

Apocynin exerts neuroprotective effects in fumonisin b1-induced neurotoxicity via attenuation of oxidative stress and apoptosis in an animal model.

The Fusarium verticillioides produces a mycotoxin, that is, fumonisin b1 (Fb1), which commonly infects corn and agricultural commodities. The Fb1 showed hepatotoxicity, neurotoxicity, and carcinogenicity in animals. Hence, the present investigation aimed to evaluate the effect of apocynin (AP) on Fb1-induced neurotoxic effects and its mechanism in the mice model and cell line. The male Balb/c mice, with the 6.75 mg/kg bwt of Fb1 were injected subcutaneously for 5 days to induce neurotoxicity. A significant elevation of serotonin (5-HT) was observed in mice treated with Fb1 in the whole brain showing biogenic amines may reflect Fb1 neurotoxicity, but the negatively regulated mechanisms were attenuated by the pretreatment of AP. In addition, AP pretreatment normalized apoptotic changes in histology and immunohistochemistry studies. In Western blotting studies, apoptotic genes were upregulated and oxidative stress genes were downregulated due to Fb1 treatment; while treating with AP, these gene expressions were rectified. Further cell cytotoxicity was investigated by MTT and lactate dehydrogenase (LDH) assays in SH-SY5Y cell line. MTT and LDH assays indicated the IC50 value to be 150 µM of Fb1, which was protected by 100 µg of AP. The electron microscopy evaluated the Fb1-induced apoptotic conditions and its cell morphology recovery by AP. These results suggest that nicotinamide adenine dinucleotide phosphate hydrogen oxidase-mediated reactive oxygen species is the primary upstream signal leading to increased Fb1-mediated neurotoxicity in mice. The use of the antioxidant AP reversed the toxin-induced oxidative stress and apoptosis by its antioxidant potency.

Attenuation of Hyperlipidemia by Medicinal Formulations of Emblica officinalis Synergized with Nanotechnological Approaches.

The ayurvedic herb Emblica officinalis (E. officinalis) is a gift to mankind to acquire a healthy lifestyle. It has great therapeutic and nutritional importance. Emblica officinalis, also known as Indian gooseberry or Amla, is a member of the Euphorbiaceae family. Amla is beneficial for treating illnesses in all its forms. The most crucial component is a fruit, which is also the most common. It is used frequently in Indian medicine as a restorative, diuretic, liver tonic, refrigerant, stomachic, laxative, antipyretic, hair tonic, ulcer preventive, and for the common cold and fever. Hyperlipidemia is also known as high cholesterol or an increase in one or more lipid-containing blood proteins. Various phytocompounds, including polyphenols, vitamins, amino acids, fixed oils, and flavonoids, are present in the various parts of E. officinalis. E. officinalis has been linked to a variety of pharmacological effects in earlier studies, including hepatoprotective, immunomodulatory, antimicrobial, radioprotective, and hyperlipidemic effects. The amla-derived active ingredients and food products nevertheless encounter challenges such as instability and interactions with other food matrices. Considering the issue from this perspective, food component nanoencapsulation is a young and cutting-edge field for controlled and targeted delivery with a range of preventative activities. The nanoformulation of E. officinalis facilitates the release of active components or food ingredients, increased bioaccessibility, enhanced therapeutic activities, and digestion in the human body. Accordingly, the current review provides a summary of the phytoconstituents of E. officinalis, pharmacological actions detailing the plant E. officinalis's traditional uses, and especially hyperlipidemic activity. Correspondingly, the article describes the uses of nanotechnology in amla therapeutics and functional ingredients.