RESUMO
Gel chromatography on Spheron P-500 of mouse serum yielded fractions elevating lactate dehydrogenase activity. The order of elution was viral activity, proteins and lactate dehydrogenase activity. Viral contamination occurred also in certain fractions subjected to repeated gel chromatography in which no proteins were detectable.
Assuntos
L-Lactato Desidrogenase/isolamento & purificação , Vírus Elevador do Lactato Desidrogenase/isolamento & purificação , Animais , Sangue/microbiologia , Proteínas Sanguíneas/isolamento & purificação , Cromatografia em Gel , L-Lactato Desidrogenase/sangue , CamundongosRESUMO
Absorption properties of hydroxyalkyl methacrylate gels containing various amounts of mercuri derivatives of p-acetaminophenoxyethyl methacrylates (Hg-APEMA) and methacrylanilide (Hg-MAA) were investigated by means of a reduced Ellman's agent (5,5'-dithiobis-2-nitrobenzoic acid) and papain. The optimum gel was used for the affinity chromatography of SH-protease from beans and for the isolation of a peptide with a free sulphhydryl group from the chymotryptic hydrolyzate of serum albumin.