RESUMO
To assess the two most toxicologically relevant species of As, namely arsenite (As(III)) and arsenate (As(V)), chromatographic separations often require two separate chromatographic columns to address the co-elution of arsenobetaine (AsB) with As(III). This issue is typically observed using conventional isocratic methods on anion exchange columns, increasing cost and analysis time. Here, we optimize the extraction of inorganic As from a lichen air biomonitor and develop an isocratic method for the chromatographic separation of five common As species on a PRP X-100 anion exchange column, resulting in the complete baseline separation of all species under study. This method was then applied to lichen biomonitors from an urban and rural site to demonstrate its use. In order of abundance, the various arsenic species in lichens from the urban site in South Africa were As(V) > As(III) > AsB > dimethylarsinic acid (DMA) > monomethylarsonic acid (MMA), and As(V) > AsB > As(III) > DMA > MMA for the rural site, where MMA was present in extremely low, non-quantifiable concentrations in lichens from both sites. Total concentrations of As were higher in samples from the urban site (6.43 ± 0.25 µg/g) than in those from the rural site (1.87 ± 0.05 µg/g), with an overall extraction efficiency of 19% and 40%, respectively. The optimized method utilized relatively inexpensive solvents and is therefore low-cost and eco-friendly in comparison with conventional chromatographic techniques. This is the first study which addresses the optimized extraction and characterization of As species in a South African lichen biomonitor of air pollution. Graphical abstract .
Assuntos
Arseniatos/análise , Arsenicais/análise , Arsenitos/análise , Monitoramento Biológico/métodos , Líquens/química , Monitoramento Biológico/instrumentação , Ácido Cacodílico/análise , Cromatografia Líquida de Alta Pressão/métodos , Espectrometria de Massas/métodos , África do Sul , UrbanizaçãoRESUMO
In recent years, silver nanoparticles (AgNPs) have attracted significant attention in medicinal, biomedical, and pharmaceutical research owing to their valuable physicochemical and antibacterial properties. Leaf sap extract (LSE) from Aloe arborescens can be used as an active ingredient for different biological applications, including wound healing. In this study, we have investigated the use of LSE from A. arborescens as a reducing, stabilizing and capping agent to produce AgNPs during the so called "green synthesis" (G-AgNPs). The objective of this study was to prepare, characterize and evaluate the potential of G-AgNPs against human pathogenic bacteria for the intended use as treatment of infected wounds. When the mixture of silver nitrate solution and LSE was exposed to direct sunlight it yielded a rapid color change from colorless to reddish-brown, indicating the formation of G-AgNPs. Physicochemical characterization such as Single particle inductively coupled plasma mass spectrometry, High resolution transmission electron microscopy and surface chemistry studies (Fourier transform infrared spectroscopy and X-Ray diffraction) revealed a small size in the range of 38±2nm, smooth surface and existence of LSE on the G-AgNPs. G-AgNPs possessed good antibacterial activity against both Pseudomonas aeruginosa and Staphylococcus aureus. The flow cytometry study revealed the increased percentage of dead cells treated by G-AgNPs through cell membrane damage, and it was further confirmed by confocal laser scanning microscopy. Thus, the present study reveals that the novel G-AgNPs demonstrated effective antibacterial properties against both Gram-negative and Gram-positive bacterial strains and shows great potential for its use in the treatment of pathogen infected wounds.