RESUMO
Computer analysis of listeria genome isolates of sequences from EMBL, GenBank, DDBJ data bases has been made. Variable and highly conservative (homology degree is 90-100% for all known isolates) genes loci iap and listeriolysin (cytolysin) gene locuses have been determined. Primer sets for detection and differentiation of Listeria species by polymerase chain reaction (PCR) were designed by computer and following thermodynamic analysis. Primer sets can provide detection of Listeria monocytogenes, L. ivanovii, L. seeligeri, L. grayi, L. innocua, L. welshimeri and detection of only pathogenic L. monocytogenes and Listeria species with listeriolysin (cytolysin) gene. Differentiation of 6 Listeria species can be done by means of two primer sets for iap and listeriolysin genes. Amplified fragments of listeria species DNA have different amplicon size. Primers melting temperatures selection allows one to carry out listeria species typing by multiplex PCR in a single tube.
Assuntos
Toxinas Bacterianas , Primers do DNA , Genoma Bacteriano , Listeria/genética , Proteínas de Bactérias/genética , Sequência de Bases , Genes de Partícula A Intracisternal , Genótipo , Proteínas de Choque Térmico/genética , Proteínas Hemolisinas , Listeria/química , Listeria/isolamento & purificação , Dados de Sequência Molecular , Reação em Cadeia da Polimerase/métodos , Alinhamento de Sequência , Homologia de SequênciaRESUMO
The results of study of the antiviral activity and pharmacokinetics of phenylimide of cis-aconitic acid (PCAA) is presented. The 20% increase of the antiviral activity of PCAA incorporated into liposomes in comparison with the antiviral activity of the pure substance was shown. Liposomes with PCAA were tropic to lymphocytes and macrophages with maximum fluorescence being observed in the spleen, while empty liposomes were accumulated mainly in the liver. After the treatment with liposomal PCAA the symptoms of herpetic meningoencephalitis became less severe with 100% survival of the experimental animals. In the control group of rabbits 50% of the animals died, and in the surviving animals blindness or paralysis developed.