[Immunobiological properties of vp24 protein of Ebola virus expressed by recombinant vaccinia virus]. / Izuchenie immunobiologicheskikh svoistv belka vp24 virusa Ebola v sostave rekombinantnogo virusa ospovaktsiny.

Immunological and biochemical parameters were studied in guinea pigs immunized with recombinant vaccinia virus containing full-sized gene of Ebola virus vp24 protein and then infected with virulent strain of Ebola virus. The majority of the studied parameters changed similarly in guinea pigs immunized with recombinant vaccinia virus and control guinea pigs inoculated with vaccinia virus both before and after challenge with Ebola virus. However, in animals immunized with recombinant vaccinia virus producing vp24 some biochemical parameters, the mean life span after challenge with Ebola virus, the level of antibodies to the virus, and the phagocytic activity of neutrophils indicated the development of immunological processes other than in controls, namely, the development of immune response to vp24. Although these processes did not eventually lead to the survival of animals, they prolonged the mean life span and resulted in the production of anti-Ebola antibodies, though the level thereof was low. These data demonstrate that recombinant vaccines against Ebola fever are a promising trend of research.

[Attempts to develop a vaccine against Ebola fever]. / Popytka polucheniia vaktsiny protiv likhoradki Ebola.

Data on the immunopathogenesis of Ebola fever in laboratory animals are presented and the efficacy of some methods of vaccine prophylaxis discussed. Antiviral immunity induced in guinea pigs by injection of inactivated viral agents did not protect them from infection, whereas injections of a nonlethal strain of the virus in ascending doses led to the formation of immunity preventing the development of disease upon inoculation with a lethal strain in high doses. The role of some viral peptides in the development of immune response is shown and variants of recombinant constructions for the prevention of Ebola fever are offered.

[Composition and immunochemical properties of goat immunoglobulins against the Ebola virus]. / Sostav i immunokhimicheskie svoistva kzo'ikh immunoglobulinov protiv virusa ébola.

Serum samples containing Ebola Virus neutralizing antibodies were prepared by prolonged immunization of goats with 10% liver homogenate from guinea pigs infected with Ebola virus. Differences in IgG fractions of normal and hyperimmune caprine blood sera were detected. Analytical chromatography on Polysil SA and immunodiffusion showed the presence of three IgG-containing fractions in hyperimmune sera. Immunochemical properties of these fractions were studied by solid-phase enzyme immunoassay and neutralization test. Antibodies to viral antigens were referred to IgG2 and IgG1a, and virus neutralizing properties were found mainly in IgG2 antibodies. Immunoglobulins were isolated from hyperimmune serum by alcohol sedimentation. The principal IgG fraction was found to contain much lower levels of antibodies to guinea pig liver antigens than intact serum, but at the same time it was characterized by a higher neutralization index.