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1.
J Dairy Sci ; 97(9): 5623-36, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24997667

RESUMO

The aim of this study was to compare 2 dry-cow management strategies and evaluate the effect of shortened dry period strategy on feed intake, metabolism, and postpartum performance of dairy cows in early lactation. Twenty-nine high-yielding dairy cows were divided into 2 groups. The control (CON) group (n=14) was assigned to a traditional dry period of approximately 60 d (57±5.9 d) and was fed a far-off dry cow ration from dry-off to -21 d relative to expected parturition. From d -21 relative to expected parturition, the cows were switched to a precalving ration containing an additional 3kg of concentrates. The cows of the experimental group (n=15) were assigned to a shortened dry period (SDP; 35±6.3 d) and were continuously fed a late-lactation diet from d -60 d relative to expected parturition until calving. After calving, both groups were fed the same lactation diet corresponding to their lactation requirements and cows were followed for 100 d of lactation. Prepartum dry matter intake of the cows assigned to an SDP and fed a late-lactation diet was approximately 4.11kg/cow per day greater compared with the CON group during the 60 d. However, no effect of dry period strategy on postpartum dry matter intake was detected. The cows with an SDP produced approximately 2.78kg/d (6.9%) less milk in the first 100 d of lactation than CON cows; the difference was not statistically significant. No differences were observed in live body weight, body condition score, or back-fat thickness between the treatments. Similarly, no differences existed in concentrations of plasma metabolites. The cows of the SDP group showed lower pH and increased concentrations of lactic acid and volatile fatty acids prepartum than the CON cows. Postpartum concentrations of lactic acid, volatile fatty acids, and NH3 and pH in rumen fluid did not differ between the treatments. Shortening of the dry period did not affect the colostrum quality or birth weights of the calves. Based on the results of this study, a traditional dry period management strategy appeared to be more favorable, considering the dry matter intake and milk production, compared with an SDP and feeding a late-lactation diet throughout the dry period.


Assuntos
Bovinos , Indústria de Laticínios/métodos , Dieta/veterinária , Lactação/fisiologia , Fenômenos Fisiológicos da Nutrição Animal , Animais , Ácidos Graxos Voláteis/análise , Feminino , Ácido Láctico/análise , Leite , Parto , Período Pós-Parto , Fatores de Tempo
2.
Folia Microbiol (Praha) ; 52(1): 99-104, 2007.
Artigo em Inglês | MEDLINE | ID: mdl-17571805

RESUMO

Bacterial 16S rDNA from fecal samples of two calves were amplified by PCR and analyzed by denaturing gradient gel electrophoresis; selected bands were sequenced. Escherichia coli and Bifidobacterium animalis were the initial colonizers, followed by species closely related to the genera Bacteroides, Clostridium and Faecalibacterium. Change of diet was connected with shifts of bacterial population and with the occurrence of many bacterial species that have not been cultured up to now. The diet change corresponded with an alteration in a volatile-fatty-acid concentration in fecal samples.


Assuntos
Animais Recém-Nascidos , Bactérias/classificação , Bactérias/crescimento & desenvolvimento , Bovinos , Trato Gastrointestinal/microbiologia , Reação em Cadeia da Polimerase/métodos , Animais , Bactérias/genética , Bactérias/isolamento & purificação , Bifidobacterium/genética , Bifidobacterium/crescimento & desenvolvimento , Bifidobacterium/isolamento & purificação , DNA Bacteriano/análise , DNA Ribossômico/análise , Ecossistema , Eletroforese/métodos , Escherichia coli/genética , Escherichia coli/crescimento & desenvolvimento , Escherichia coli/isolamento & purificação , Fezes/microbiologia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
3.
Meat Sci ; 76(3): 517-23, 2007 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22060995

RESUMO

Forty-eight heifers, Limousin (LI) and Charolais (CH) breed, were used to evaluate the effect of a basal mixed diet with linseed supplementation (108g/kg DM) on performance and fatty acid (FA) composition of M. longissimus thoracis (MLT) and subcutaneous fat. The feed intake and weight gains were higher, and feed per gain ratio lower in CH heifers than in LI heifers. The muscle lipids and subcutaneous fat of LI heifers contained significantly more MUFA, PUFA, CLA, and less SFA than the lipids of CH heifers. The feeding of extruded linseed significantly increased the concentration of linolenic acid (C18:3n-3), CLA, and decreased PUFA n-6 to PUFA n-3 ratio in lipids extracted from both tissues. Linseed supplementation decreased SFA and increased PUFA in subcutaneous fat. In MLT lipids of linseed-fed heifers PUFA of the n-3 series were increased. It can be concluded that the growth performance of LI heifers was lower, but that their lipids contained more FA that are thought to be important for human health. The feeding of linseed at 108g/kg DM enhanced the nutritive value of beef in terms of FA profile. Breed differences were generally more important than effects of the diet. Dietary effects, however, were more pronounced in PUFA n-3 and arachidonic acid (C20:4n-6).

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