Connections between the Levant and the Balkans in the late Middle Pleistocene: Archaeological findings from Velika and Mala Balanica Caves (Serbia).

Major changes in the technological, economic, and social behavior of Middle Pleistocene hominins occurred at the onset of the Middle Paleolithic, 400-200 ka. However, until recently it was not possible to establish when, where, and how certain forms of Middle Paleolithic behavior appeared and spread into Southeastern Europe, mainly owing to gaps in the Paleolithic record. Here we report new results of dating, material culture, and the archaeological context of finds from the Balanica Cave Complex in Sicevo (Serbia). Two methods-thermoluminescence and electron spin resonance-were used to date the sequence. The geoarchaeological context was examined through sedimentology, micromorphology, and spatial analysis. Microfaunal remains were used to constrain the dates within an ecological zone, whereas macrofauna was analyzed for taxonomy and taphonomy to examine the source of accumulation and hominin behavior. Technological and typological features of the lithic assemblage were used to characterize lithic production at the site. Materials recovered from Layer 3 in Velika Balanica and from Layer 2 in Mala Balanica, both dated to MIS 9-7, include a distinctive set of archaeological assemblages which resemble contemporaneous Yabrudian assemblages from the Levant in important ways, and which are unlike contemporary material from the surrounding regions. In Velika Balanica, the lithic assemblages are associated with a large fireplace containing evidence of human activities similar to those from Qesem Cave (Israel). Dental remains uncovered in the same layer are consistent with Neanderthals. These findings suggest that the end of the Middle Pleistocene (before 300-240 ka) saw population movement and/or cultural transmission between Southwest Asia and the Balkans, which led eventually to a transfer of technology between Middle Eastern and European hominin populations and contributed to the shaping of Neanderthal behaviors throughout the eastern and northern Mediterranean.

Deep ancestry of collapsing networks of nomadic hunter-gatherers in Borneo.

Theories of early cooperation in human society often draw from a small sample of ethnographic studies of surviving populations of hunter-gatherers, most of which are now sedentary. Borneo hunter-gatherers (Punan, Penan) have seldom figured in comparative research because of a decades-old controversy about whether they are the descendants of farmers who adopted a hunting and gathering way of life. In 2018 we began an ethnographic study of a group of still-nomadic hunter-gatherers who call themselves Punan Batu (Cave Punan). Our genetic analysis clearly indicates that they are very unlikely to be the descendants of neighbouring agriculturalists. They also preserve a song language that is unrelated to other languages of Borneo. Dispersed travelling groups of Punan Batu with fluid membership use message sticks to stay in contact, co-operate and share resources as they journey between rock shelters and forest camps. Message sticks were once widespread among nomadic Punan in Borneo, but have largely disappeared in sedentary Punan villages. Thus the small community of Punan Batu offers a rare glimpse of a hunting and gathering way of life that was once widespread in the forests of Borneo, where prosocial behaviour extended beyond the face-to-face community, facilitating successful collective adaptation to the diverse resources of Borneo's forests.

Early Middle Stone Age personal ornaments from Bizmoune Cave, Essaouira, Morocco.

Ornaments such as beads are among the earliest signs of symbolic behavior among human ancestors. Their appearance signals important developments in both cognition and social relations. This paper describes and presents contextual information for 33 shell beads from Bizmoune Cave (southwest Morocco). Many of the beads come as deposits dating to ≥142 thousand years, making them the oldest shell beads yet recovered. They extend the dates for the first appearance of this behavior into the late Middle Pleistocene. The ages and ubiquity of beads in Middle Stone Age (MSA) sites in North Africa provide further evidence of the potential importance of these artifacts as signals of identity. The early and continued use of Tritia gibbosula and other material culture traits also suggest a remarkable degree of cultural continuity among early MSA Homo sapiens groups across North Africa.

The transplant cohort of the German center for infection research (DZIF Tx-Cohort): study design and baseline characteristics.

Infectious complications are the major cause of morbidity and mortality after solid organ and stem cell transplantation. To better understand host and environmental factors associated with an increased risk of infection as well as the effect of infections on function and survival of transplanted organs, we established the DZIF Transplant Cohort, a multicentre prospective cohort study within the organizational structure of the German Center for Infection Research. At time of transplantation, heart-, kidney-, lung-, liver-, pancreas- and hematopoetic stem cell- transplanted patients are enrolled into the study. Follow-up visits are scheduled at 3, 6, 9, 12 months after transplantation, and annually thereafter; extracurricular visits are conducted in case of infectious complications. Comprehensive standard operating procedures, web-based data collection and monitoring tools as well as a state of the art biobanking concept for blood, purified PBMCs, urine, and faeces samples ensure high quality of data and biosample collection. By collecting detailed information on immunosuppressive medication, infectious complications, type of infectious agent and therapy, as well as by providing corresponding biosamples, the cohort will establish the foundation for a broad spectrum of studies in the field of infectious diseases and transplant medicine. By January 2020, baseline data and biosamples of about 1400 patients have been collected. We plan to recruit 3500 patients by 2023, and continue follow-up visits and the documentation of infectious events at least until 2025. Information about the DZIF Transplant Cohort is available at https://www.dzif.de/en/working-group/transplant-cohort .

Intra-assemblage variation in the macro-blade assemblage from the 1963 excavation at Shuidonggou locality 1, northern China, in the context of regional variation.

The emergence of the Upper Paleolithic and regional variability in early Upper Paleolithic industries are prominent topics in Paleolithic archaeology, with special relevance to the dispersal and differentiation of early modern human cultures across Eurasia. The so-called Initial Upper Paleolithic (IUP) has been considered a key element in the emergence of the Upper Paleolithic in northern Asia. Here, we examine the intra-assemblage variation in the collection from the 1963 excavation at Shuidonggou locality 1, a major IUP site in northern China. We combine technological and quantitative attribute analyses to investigate the variety of core reduction sequences and tool manufacture behaviors at the site. A range of core reduction sequences have been documented at Shuidonggou locality 1, including both simple core reduction and prepared core reduction yielding laminar (blade-like) products. The simple core reduction component may due to mixed non-IUP assemblages from different archaeological layers. Among the laminar core reduction sequences, the main strategy involves asymmetrical exploitation of the broad face of core blank, producing blades and elongate flakes, and resembling a recurrent Levallois blade method sensu lato. We compare Shuidonggou laminar blank production with that of IUP assemblages in the Siberian Altai, northern Mongolia, and the Transbaikal region. The comparison demonstrates a general consistency to the basic blank production in IUP assemblages across northern Asia, with some regional variation. The results suggest a multi-directional model of diffusion of the IUP in northeast Asia.

The evolution of raw material procurement strategies: A view from the deep sequence of Tabun Cave, Israel.

Changes in the ways Paleolithic foragers exploited raw material sources are linked to mobility, the demands of production, and investment in quarrying. Here, we analyze the use of raw materials in a long series of superimposed layers from Tabun Cave dating to the Middle Pleistocene, attributed to the Lower and Middle Paleolithic periods. Using the cortex preserved on the surfaces of artifacts, including blanks, tools and cores, we distinguished between flints obtained from primary and secondary geological contexts. The results from Tabun Cave indicate that the exploitation of secondary sources was fairly common during the earlier part of the Lower Paleolithic sequence. It decreased during the later part of the Acheulo-Yabrudian complex of the Lower Paleolithic, coinciding with growing use of predetermined technological strategies, which demand high-quality raw materials. By the Middle Paleolithic, primary and secondary raw materials are generally designated for different reduction trajectories, suggesting a growing distinction and formalization of technological strategies. The need for the 'best' stone for Middle Paleolithic laminar and Levallois production may have necessitated increased investment in raw material procurement. During most of the Lower Paleolithic, raw material needs could have been met easily through a purely embedded strategy, in which raw material was collected while focusing on other activities. Starting in the late Acheulo-Yabrudian and especially during the Middle Paleolithic, the focus on primary geological contexts may have demanded greater planning of visits to raw material outcrops. In other words, in the Middle Paleolithic and possibly already during the very end of the Lower Paleolithic, raw material procurement had greater influence on patterns of movement through the landscape.

The easternmost Middle Paleolithic (Mousterian) from Jinsitai Cave, North China.

The dispersal of Neanderthals and their genetic and cultural interactions with anatomically modern humans and other hominin populations in Eurasia are critical issues in human evolution research. Neither Neanderthal fossils nor typical Mousterian assemblages have been reported in East Asia to date. Here we report on artifact assemblages comparable to western Eurasian Middle Paleolithic (Mousterian) at Jinsitai, a cave site in North China. The lithic industry at Jinsitai appeared at least 47-42 ka and persisted until around 40-37 ka. These findings expand the geographic range of the Mousterian-like industries at least 2000 km further to the east than what has been previously recognized. This discovery supplies a missing part of the picture of Middle Paleolithic distribution in Eurasia and also demonstrates the makers' capacity to adapt to diverse geographic regions and habitats of Eurasia.

What moves us? How mobility and movement are at the center of human evolution.

Movement is central to the survival of all free-living organisms. Consequently, movement and what anthropologists often refer to as mobility, which is the sum of small-scale movements tracked across larger geographic and temporal scales, are key targets of selection. Movement and mobility also underpin many of the key features that make us human and that allowed our lineage to adapt to changing environments across the globe. The most obvious example is the evolution of humans' singular mode of locomotion. Bipedalism is arguably the most important derived anatomical trait of the hominin lineage. The mechanisms and circumstances that gave rise to this novel mode of movement remain subjects of intense research.

'Fire at will': the emergence of habitual fire use 350,000 years ago.

The use of fire is central to human survival and to the processes of becoming human. The earliest evidence for hominin use of fire dates to more than a million years ago. However, only when fire use became a regular part of human behavioral adaptations could its benefits be fully realized and its evolutionary consequences fully expressed. It remains an open question when the use of fire shifted from occasional and opportunistic to habitual and planned. Understanding the time frame of this 'technological mutation' will help explain aspects of our anatomical evolution and encephalization over the last million years. It will also provide an important perspective on hominin dispersals out of Africa and the colonization of temperate environments, as well as the origins of social developments such as the formation of provisioned base camps. Frequencies of burnt flints from a 16-m-deep sequence of archaeological deposits at Tabun Cave, Israel, together with data from the broader Levantine archaeological record, demonstrate that regular or habitual fire use developed in the region between 350,000-320,000 years ago. While hominins may have used fire occasionally, perhaps opportunistically, for some million years, we argue here that it only became a consistent element in behavioral adaptations during the second part of the Middle Pleistocene.

Predetermined flake production at the Lower/Middle Paleolithic boundary: Yabrudian scraper-blank technology.

While predetermined débitage technologies are recognized beginning with the middle Acheulian, the Middle Paleolithic is usually associated with a sharp increase in their use. A study of scraper-blank technology from three Yabrudian assemblages retrieved from the early part of the Acheulo-Yabrudian complex of Tabun Cave (ca. 415-320 kyr) demonstrates a calculated and preplanned production, even if it does not show the same complexity and elaboration as in the Levallois technology. These scraper dominated assemblages show an organization of production based on an intensive use of predetermination blank technology already in place at the end of the Lower Paleolithic of the Levant. These results provide a novel perspective on the differences and similarities between the Lower and Middle Paleolithic industries. We suggest that there was a change in the paradigm in the way hominins exploited stone tools: in many Middle Paleolithic assemblages the potential of the stone tools for hafting was a central feature, in the Lower Paleolithic ergonometric considerations of manual prehension were central to the design of blanks and tools.

A forager-herder trade-off, from broad-spectrum hunting to sheep management at Asikli Höyük, Turkey.

Asikli Höyük is the earliest known preceramic Neolithic mound site in Central Anatolia. The oldest Levels, 4 and 5, spanning 8,200 to approximately 9,000 cal B.C., associate with round-house architecture and arguably represent the birth of the Pre-Pottery Neolithic in the region. Results from upper Level 4, reported here, indicate a broad meat diet that consisted of diverse wild ungulate and small animal species. The meat diet shifted gradually over just a few centuries to an exceptional emphasis on caprines (mainly sheep). Age-sex distributions of the caprines in upper Level 4 indicate selective manipulation by humans by or before 8,200 cal B.C. Primary dung accumulations between the structures demonstrate that ruminants were held captive inside the settlement at this time. Taken together, the zooarchaeological and geoarchaeological evidence demonstrate an emergent process of caprine management that was highly experimental in nature and oriented to quick returns. Stabling was one of the early mechanisms of caprine population isolation, a precondition to domestication.

Early upper paleolithic shell beads at Üçagizli Cave I (Turkey): technology and the socioeconomic context of ornament life-histories.

Ten early Upper Paleolithic layers in Üçagizli Cave I (41-29 uncalibrated ky BP) on the Hatay coast of southern Turkey preserve a rich and varied record of early Upper Paleolithic life, including the production and use of large numbers of shell ornaments. This study examines shell bead production, use, and discard in relation to site function and the diversity of on-site human activities. Four factors are expected to contribute to variation in the ornament assemblages, one environmental and three behavioral. The behavioral factors relate to winnowing for quality as a function of distance from the raw material source, changes in the size of user groups, and symbol standardization. The accumulation rates for shell beads, bones, and stone tools paralleled one another through time, indicating that ornament discard followed the pulse of daily life at this site. All stages of manufacture and use are well represented in each assemblage, and half or more of the ornaments show evidence of extended use. Changes in the local marine environment do not explain much of the variation in the assemblages, pointing instead to behavioral causes. The richness of shell types that were collected as raw material correlates to greater exploitation of edible marine shellfish and greater occupation intensity. Much of this variation in the ornament raw material was eliminated during the manufacture stage, almost certainly reflecting the influence of cultural norms. A focus on basket-shaped shells changed remarkably little over thousands of years, despite significant changes in other domains of technology. This last result suggests that beads were the most irreducible and conservative elements of more complex design traditions.

Re-examination of the dates of large blade technology in China: a comparison of Shuidonggou Locality 1 and Locality 2.

The presence and age of large blade technology at the Shuidonggou site is a pivotal issue in discussions of the spread of blade technology in East Eurasia. Madsen and colleagues' influential work uses the dates (24,000-29,000 rcy BP [radiocarbon years before present]) they obtained from Shuidonggou Locality 2 to estimate the age of blade technology in this region, and suggested a very late arrival of Levallois-like blade technology from the north. This paper re-examines the evidence for the age of blade technology at Shuidonggou by comparing the lithic assemblages from the new excavations at Locality 2 with those from Locality 1. Several important points are demonstrated: (1) the lithic industry of cultural layers 1 through 4 at Locality 2 is not based on large blades, so reported dates from these layers cannot be an indicator of the age of large blade technology; (2) comparing Locality 1 and 2, the age of large blade technology appears to be around 34,000-38,000 calendar years BP (before present) in this region, suggesting a relatively rapid technology dispersal from the west and/or north; (3) the so-called 'Shuidonggou lower cultural layer' at Locality 1 includes both large blade and simple flake industries.

Modeling effects of local extinctions on culture change and diversity in the paleolithic.

The persistence of early stone tool technologies has puzzled archaeologists for decades. Cognitively based explanations, which presume either lack of ability to innovate or extreme conformism, do not account for the totality of the empirical patterns. Following recent research, this study explores the effects of demographic factors on rates of culture change and diversification. We investigate whether the appearance of stability in early Paleolithic technologies could result from frequent extinctions of local subpopulations within a persistent metapopulation. A spatially explicit agent-based model was constructed to test the influence of local extinction rate on three general cultural patterns that archaeologists might observe in the material record: total diversity, differentiation among spatially defined groups, and the rate of cumulative change. The model shows that diversity, differentiation, and the rate of cumulative cultural change would be strongly affected by local extinction rates, in some cases mimicking the results of conformist cultural transmission. The results have implications for understanding spatial and temporal patterning in ancient material culture.

The early Upper Paleolithic occupations at Uçagizli Cave (Hatay, Turkey).

This paper summarizes results from excavations at Uçagizli Cave (Hatay, Turkey) between 1999 and 2002 and 2005. This collapsed karstic chamber contains a sequence of early Upper Paleolithic deposits that span an interval between roughly 29,000 and 41,000 (uncalibrated) radiocarbon years BP. Lithic assemblages can be assigned to two major chronostratigraphic units. The earliest assemblages correspond with the Initial Upper Paleolithic, whereas the most recent ones fit within the definition of the Ahmarian. Substantial assemblages of stone tools, vertebrate faunal remains, ornaments, osseous artifacts, and other cultural materials provide an unusually varied picture of human behavior during the earliest phases of the Upper Paleolithic in the northern Levant. The sequence at Uçagizli Cave documents the technological transition between Initial Upper Paleolithic and Ahmarian, with a high degree of continuity in foraging and technological activities. The sequence also documents major shifts in occupational intensity and mobility.

Kaletepe Deresi 3 (Turkey): archaeological evidence for early human settlement in Central Anatolia.

Located in the Central Anatolian Volcanic Province, Kaletepe Deresi 3 was discovered in the summer of 2000 and has been under investigation since that time. Volcanic activity in the region generated a number of obsidian intrusions that have attracted humans to the area throughout prehistory. The stratigraphic sequence at Kaletepe Deresi 3, more than 7 m in depth, presents a series of archaeological horizons representing the Lower and Middle Paleolithic. The site contains the longest open-air Paleolithic sequence excavated in Turkey, as well as the first in situ Acheulean industry documented in Anatolia. Tephras in the upper Middle Paleolithic horizons and the rhyolithic bedrock bracket the timespan represented at Kaletepe Deresi 3. The lithic industry at the site illustrates a wide range of technological behaviors and documents changes in raw-material exploitation and artifact manufacture through the Lower and Middle Paleolithic.

4,300-year-old chimpanzee sites and the origins of percussive stone technology.

Archaeological research in the African rainforest reveals unexpected results in the search for the origins of hominoid technology. The ancient Panin sites from Côte d'Ivoire constitute the only evidence of prehistoric ape behavior known to date anywhere in the world. Recent archaeological work has yielded behaviorally modified stones, dated by chronometric means to 4,300 years of age, lodging starch residue suggestive of prehistoric dietary practices by ancient chimpanzees. The "Chimpanzee Stone Age" pre-dates the advent of settled farming villages in this part of the African rainforest and suggests that percussive material culture could have been inherited from an common human-chimpanzee clade, rather than invented by hominins, or have arisen by imitation, or resulted from independent technological convergence.