Clinical evaluation on the use of a new accessory device for Fox plane.

OBJECTIVE: Evaluate the assimilation capacity and ease of handling of the Fox plane accessory by dentistry students, through a questionnaire about the experience in using this device compared to the conventional one. Its intention is to minimize possible interpretation errors and challenges that the traditional method determines. METHODS: After approval by the Research Ethics Committee, registration at Sistema Nacional de Informações sobre Ética em Pesquisa/National Information System on Research Ethics (SISNEP) and signing of the free and informed consent form, 51 undergraduate students treating patients in need of complete dentures at Instituto de Ciência e Tecnologia/Institute of Science and Technology (ICT) Unesp in São José dos Campos completed a questionnaire to evaluate the technical ease of use and provider acceptance. The obtained data were submitted to statistical analysis, evaluating technical ease and acceptance by operators. RESULTS: The results were analyzed using descriptive and inferential statistics using the Jamovi 2.2.5 software. From the responses, the frequency was obtained for each question in the questionnaire, then the weighted mean was calculated, average rating and percentage. With the results of these analyses it was possible to measure the level of satisfaction of the participants in relation to the use of the device. CONCLUSION: It was concluded that most students have difficulty using the conventional Fox plane and that the accessory facilitated the process of determining the superior orientation plane, and its ease of use.

Chlorinated-based bioceramics incorporated in polycaprolactone membranes.

The development of bioactive membranes with bone repair properties is great interest in the field of tissue engineering. In this study, we aimed to fabricate and characterize a composite membrane composed of sol-gel synthesized bioceramics and electrospun polycaprolactone (PCL) fibers for bone tissue regeneration applications. The bioceramics were prepared using the sol-gel method with nitrate (N) and chloride (CL) as precursors. PCL and bioceramic solutions were electrospun to obtain ultrafine fiber mats. Raman spectroscopy, x-ray diffraction (XRD), Fourier Transform Infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM) were used to characterize the materials. The results showed that both chlorinated and non-chlorinated bioceramics contained NBOs (non-bridge bonds) and crystallized the α-wollastonite phase, with the chlorinated version doing so at lower temperatures. In vitro tests were performed to evaluate cytotoxicity, cell adhesion, and mineralized matrix formation on the membranes. The composite membranes showed improved cell viability and promoted mineralization nodules formation. This study presents a promising approach for the development of bioactive membranes for bone tissue engineering, with potential applications in bone regeneration therapies.

Electrospun polylactic acid scaffolds with strontium- and cobalt-doped bioglass for potential use in bone tissue engineering applications.

The development of nanoscale biomaterials associated with polymers has been growing over the years, due to their important structural characteristics for applications in biological systems. The present study aimed to produce and test polymeric scaffolds composed of polylactic acid (PLA) fibers associated with a 58S bioglass doped with therapeutic ions for use in tissue engineering. Three 58S Bioglass was obtained by the sol-gel route, pure and doped with 5% strontium and cobalt ions. Solutions of 7% PLA was used as control and added the three different bioglass, 4% of 58S bioglass (PLA-BG), 4% bioglass-doped strontium (PLA-BGSr) and 4% bioglass-doped cobalt (PLA-BGCo). Scaffolds were produced through electrospinning process, and was characterized chemical and morphologically. The in vitro tests were performed using mesenchymal cells cultures from femurs of nine rats, grown in osteogenic supplemented total culture medium. After osteoblastic differentiation induction cell viability, alkaline phosphatase activity, total protein content quantification, and visualization of mineralization nodule tests were performed. Analysis of normal distribution used the Shapiro-Wilk test (nanofibers diameter and biological assay). Data were compared using the Kruskal-Wallis nonparametric test (p = 0.05). The bioglasses produced proved to be free of nitrate, chlorinated and nano-sized, with effective incorporation of therapeutic ions in their structure. All materials showed cell viability (>70%), total protein production, and alkaline phosphatase activity. It was possible to develop polylactic acid scaffolds associated with 58S bioglass doped with therapeutic ions without cytotoxicity. Scaffolds characteristics appear to sustain its application in bone tissue engineering.

Polycaprolactone/chlorinated bioglass scaffolds doped with Mg and Li ions: Morphological, physicochemical, and biological analysis.

The objective was to synthesize and characterize fine polycaprolactone (PCL) fibers associated with a new 58S bioglass obtained by the precipitated sol-gel route, produced by the electrospinning process in order to incorporate therapeutic ions (Mg and Li). In PCL/acetone solutions were added 7% pure bioglass, bioglass doped with Mg(NO3 )2 and Li2 CO3 and were subjected to electrospinning process. The fibers obtained were characterized morphologically, chemically and biologically. The results showed the presence of fine fibers at the nanometric scale and with diameters ranging from 0.67 to 1.92 µm among groups. Groups containing bioglass showed particles both inside and on the surface of the fibers. The components of the polymer, bioglass and therapeutic ions were present in the fibers produced. The produced fibers showed cell viability and induced the formation of mineralization nodules. It was observed the applicability of that methodology in making an improved biomaterial, which adds the osteoinductive properties of the bioglass to PCL and to those of therapeutic ions, applicable to guided bone regeneration.

Development of a cell phone application as an auxiliary teaching tool for the subject of complete dentures / Desenvolvimento de aplicativo de celular intitulado: "Manual de Prótese Total ­ Reabilitando sorrisos", como uma ferramenta didática auxiliar para a disciplina de prótese total

Objective: The study aimed to develop a cell phone application entitled: "Total Prosthesis Manual ­ Rehabilitating smiles", as an auxiliary teaching tool for teachers and students through digital technology, with a smartphone. Material and Methods: The tool was structured on the "Application Factory website", which allows the creation of mobile applications in different formats, with broad and interactive features on IOS and Android platforms. The expository format of the content is in slide format, containing descriptive theory and images about the stages of making a complete prosthesis; from necessary materials, photos and descriptive guidance of the steps. Results: The application is a complementary teaching resource to assist undergraduate and postgraduate students and professionals working in the area of complete prosthetics. The theoretical and practical content selected for the application covered all stages of understanding, development and possible complications associated with the manufacture of a complete bimaxillary prosthesis, from planning, impressions, models, orientation plans, tooth assembly, adaptations and delivery. Conclusion: The application provided a low-cost, expandable and easy-to-use teaching resource for teaching complete dentures. It is essential to develop various analyzes such as user experience tests, application effectiveness, development of new technologies and improvement of techniques, so that their potential for enriching learning in complete dentures and dentistry in general can be verified.(AU)

Objetivo: O estudo teve como objetivo desenvolver um aplicativo de celular intitulado: "Manual de Prótese Total ­ Reabilitando sorrisos", como uma ferramenta didática auxiliar para professores e alunos por meio da tecnologia digital, com smartphone. Material e Métodos: A ferramenta foi estruturada no "site da Fábrica de Aplicativos", que permite a criação de aplicativos móveis em diversos formatos, com recursos amplos e interativos nas plataformas IOS e Android. A modalidade expositiva do conteúdo é em formato de slides, contendo teoria descritiva e imagens sobre as etapas de confecção de uma prótese total; a partir de materiais necessários, fotos e orientação descritiva das etapas. Resultados: O aplicativo é um recurso didático complementar para auxiliar estudantes de graduação, pós-graduação e profissionais que atuam na área de prótese total. O conteúdo teórico e prático selecionado para a aplicação visou todas as etapas de compreensão, desenvolvimento e possíveis complicações associadas à confecção de uma prótese total bimaxilar, desde o planejamento, moldagens, maquetes, planos de orientação, montagem dos dentes, adaptações e entrega. Conclusão: O aplicativo trouxe um recurso didático de baixo custo, expansível e fácil de usar para o ensino de próteses totais. É fundamental desenvolver diversas análises como testes de experiência do usuário, eficácia de aplicação, desenvolvimento de novas tecnologias e aprimoramento de técnicas, de forma que possa ser verificado seu potencial de enriquecimento do aprendizado em prótese total e odontologia em geral(AU)

Nanofibras de PCL associadas ao biovidro dopado: um novo biomaterial para engenharia tecidual / PCL nanofibers associated with doped bioglass: a new biomaterial for tissue engineering

O objetivo do estudo foi sintetizar e caracterizar fibras ultrafinas de policaprolactona (PCL) associadas a um novo biovidro 58S em duas diferentes rotas de produção, produzidas pelo processo de eletrofiação a fim de selecionar a melhor rota para a incorporação de íons terapêuticos e avaliar as diferenças entre as rotas e a influência dos íons. Soluções de PCL puro 20% e contendo 7% de biovidros dopados (10% Mg(NO3)2 e 5% Li2CO3), separadamente, foram submetidas ao processo de eletrofiação, onde foram utilizados três parâmetros fixos: 2 mLh-1 de razão de fluxo, 10 kV de voltagem e 12 cm de distância. As fibras obtidas foram caracterizadas morfológica (MEV, análise do diâmetro das fibras e análise da molhabilidade), química (EDS, DRX, FTIR e Raman) e biologicamente (viabilidade celular, conteúdo de proteína total, fosfatase alcalina e formação de nódulos de mineralização). Os resultados obtidos mostraram que a melhor rota foi a sol- gel precipitado, que foram dopados com íons terapêuticos (10% Mg(NO3)2 e 5% Li2CO3). Foi observada a aplicabilidade da referida metodologia em confeccionar um biomaterial aprimorado, que agregando as propriedades osteoindutoras do biovidro ao PCL e às dos íons terapêuticos, aplicável à regeneração óssea guiada apresentando respostas físicas, químicas e biológicas favoráveis ao comportamento celular

The aim of the study was to synthesize and characterize ultrafine polycaprolactone fibers (PCL) associated with a new 58S bioglass in two different production routes, produced by the electrospinning process in order to select the best route for the incorporation of therapeutic ions and differences as a difference between the routes and the influence of the ions. Solutions of pure PCL 20% and containing 7% doped bio-glass (10% Mg (NO3) 2 and 5% Li2CO3), requirements, were submitted to the electrospinning process, where three fixed parameters were used: 2 mLh-1 flow, 10 kV voltage and 12 cm distance. The curved fibers were characterized morphological (SEM, fiber diameter analysis and wettability analysis), chemical (EDS, DRX, FTIR and Raman) and biologically (cell viability, total protein content, alkaline phosphatase and formation of mineralization nodules). The results sought that the best route for a precipitated sol-gel, which were doped with therapeutic ions (10% Mg (NO3) 2 and 5% Li2CO3). It was observed the applicability of the methodology in making an improved biomaterial, which adding the osteoinductive properties of the bioglass to the PCL and those of the therapeutic ions, applicable to guided bone regeneration presenting physical, chemical and biological responses favorable to the cellular behavior

Analysis of the association of parameters in the formation of ultrafine fibers from PEI and PMMA / Análise da associação de parâmetros na formação de fibras ultrafinas de PEI e PMMA

Objective: The aim of the study was to fabricate and morphologically characterize ultrafine Polyetherimide fibers (PEI) associated with Polymethylmethacrylate (PMMA) ­ PP (group formed by the association of PEI with PMMA), produced by the electrospinning process. Material and Methods: A solution of PEI (0.562 g) + PMMA (0.377 g) dissolved in 2.5 mL of chloroform, 0.85 mL of Dimethylformamide (DMF) and 0.85 mL of 1.1.2.2 Tetrachloroethane (TCE) was prepared. For the electrospinning process, different continuous voltages (10 to 18 kV) and two different distances (8 and 12 cm) between the needle tip and the collecting apparatus were used, giving rise to 6 distinct groups of ultrafine fibers (PP 1 to 6) that were observed in Scanning Electron Microscopy to check for defects and calculate the average diameter of the fibers. Results: The best parameter, the parameter that was most effective for the production of fibers, observed was subjected to Energy Dispersion X-ray Spectroscopy (EDS), X-ray Diffraction (XRD) and Contact Angle Analysis tests. The data were analyzed using the ANOVA and Tukey test (p <0.05). From the comparative analysis of the pre-established parameters, the pattern of PP4 ultrafine fibers was shown to be more effective. Conclusion: The PP4 standard (13 kV ­ 12 cm) had an average diameter of 0.37 µm. An adequate parameter to electrospinning was able to produce ultrafine fibers of PMMA/PEI (AU)

Introdução: O objetivo do estudo foi sintetizar e caracterizar morfologicamente fibras ultrafinas de Polieterimida (PEI) associadas ao Polimetilmetacrilato (PMMA) - PP (grupo formado pela associação de PEI com PMMA), produzidas pelo processo de eletrofiação. Material e Métodos: Foi preparada uma solução de PEI (0,562 g) + PMMA (0,377 g) dissolvido em 2,5 mL de clorofórmio, 0,85 mL de Dimetilformamida (DMF) e 0,85 mL de 1.1.2.2 Tetracloroetano (TCE). Para o processo de eletrofiação, foram utilizadas diferentes tensões contínuas (10 a 18 kV) e duas distâncias diferentes (8 e 12 cm) entre a ponta da agulha e o aparelho coletor, dando origem a 6 grupos distintos de fibras ultrafinas (PP 1 a 6) que foram observados em Microscopia Eletrônica de Varredura para verificar defeitos e calcular o diâmetro médio das fibras. Resultados: O melhor parâmetro, o parâmetro mais eficaz para a produção de fibras, observado foi submetido aos testes de Espectroscopia de Dispersão de Energia (EDS), Difração de Raios X (DRX) e Análise do Ângulo de Contato. Os dados foram analisados pela ANOVA e teste de Tukey (p <0,05). A partir da análise comparativa dos parâmetros pré-estabelecidos, o padrão das fibras ultrafinas PP4 mostrou-se mais eficaz. Conclusão: O padrão PP4 (13 kV - 12 cm) apresentou diâmetro médio de 0,37 µm. Um parâmetro adequado para eletrofiação foi capaz de produzir fibras ultrafinas de PMMA / PEI. (AU)

Development of electrospun-based polyetherimide fibers and diameter analysis for potential use in dental materials / Desenvolvimento de fibras de polieterimida à base de eletrofiação e análise de diâmetro para uso potencial em materiais odontológicos

Objetives: this study aimed to fabricate electrospun-based polyetherimide (PEI) fibers, under controlled parameters, and to perform a diameter analysis for potential mechanical improvement of dental materials. Material and Methods: PEI pallets (0.75 g) were dissolved in 2 mL of chloroform and then processed by electrospinning, under a flow rate of 1mLh-1, three different electrical voltages (10kV, 15kV and 20kV) and three distances (10 cm, 15 cm and 18 cm) between the needle tip and collector. These parameter combinations resulted in nine experimental groups that were analyzed using scanning electron microscopy (SEM) and image processing program for diameter measurement. Statistical analysis was performed using two-way ANOVA with post-hoc Tukey (5% significance). Results: from SEM images it was possible to observe formation of solid, misaligned and flawless defect-free fibers. And from the statistical analysis, distance (p = 0,0026) and the electric tension (p = 0,0012) showed a significant difference, but not for interaction between then (p = 0,4486). Conclusion: thus, it can be concluded that there is a possibility of the morphology control of PEI electrospun fibers, such as diameter, that can be used for a variety of applications such as incorporation in dental materials in order to improve its properties. (AU)

Objetivos: o objetivo deste estudo foi fabricar fibras de polieterimida à base de eletrofiação (PEI), sob parâmetros controlados, e realizar uma análise de diâmetro para potencial aprimoramento mecânico de materiais odontológicos. Material e métodos: paletes de PEI (0,75 g) foram dissolvidos em 2 mL de clorofórmio e processados pela eletrofiação, sob uma razão de fluxo de 1 mLh-1, três tensões elétricas diferentes (10 kV, 15 kV e 20 kV) e três distâncias (10 cm, 15 cm e 18 cm) entre a ponta da agulha e o coletor. Essas combinações de parâmetros resultaram em nove grupos diferentes que foram analisadas usando microscopia eletrônica de varredura (MEV) e programa de processamento de imagem para medição de diâmetro. Resultados: a partir das imagens de MEV foi possível observar a formação de fibras sólidas, desalinhadas e sem defeitos. E a partir da análise estatística, a distância (p = 0,0026) e a tensão elétrica (p = 0,0012) apresentaram diferença significativa, mas não para interação entre elas (p = 0,4486). Conclusão: assim, pode-se concluir que existe a possibilidade de controle da morfologia das fibras eletrofiadas PEI, como o diâmetro, que pode ser utilizado para uma variedade de aplicações, como incorporação em materiais dentários, a fim de melhorar suas propriedades (AU)