[Detection of antibodies to submitochondrial particles, F1-ATPase complex and liver specific lipoprotein in liver diseases using ELISA]. / Detekce protilátek proti submitochondriálnym cásticím, F1-ATP-ázovému komplexu a jaternímu specifickému lipoproteínu u jaterních onemocnení pomocí ELISA.

ELISA was elaborated for the determination of antibodies against liver antigen complexes--submitochondrial particles, F1-ATPase and liver specific lipoprotein. The parameters achieved so far allow to use the assay as an undemanding complementary laboratory technique in diagnosing and monitoring hepatopathies of autoimmune origin. Cross reactivity between individual antigen complexes was recorded in the majority of sera from positively reacting patients. The preliminary results show that individual antigen complexes have similar antigen structures bound to high-molecular membrane complexes, some of which, however, can be solubilized on maintaining antigen activity in ELISA.

Inhibition of proliferation of HeLa cells by cocultivation with early mouse embryos (preliminary report).

Eight- to 16-cell zona-free mouse embryos were cocultivated with 5 to 10 HeLa cells in cultivation drops of 0.1 microliter containing D-PBS medium supplemented with 20% fetal bovine serum. The control group included HeLa cells alone cultivated in the same medium under the same culture conditions. The two cultures were evaluated after 72 hours. The control group showed 147 out of 181 cases of proliferation of HeLa cells (81.22%), whereas HeLa cells cocultivated with zona-free mouse embryos only proliferated in 2 out of 156 cases (1.28%). Proliferation of HeLa cells was inhibited even though these were not in direct contact with the embryos. The experiments suggest that the secretory activity of early embryos in culture medium inhibits proliferation of transformed cells via a special agent.

[Relation between myoglobin levels and succinate dehydrogenase activity in muscle fibers]. / Vztahy medzi obsahom myoglobínu a aktivitou sukcinát dehydrogenázy vo svalových vláknach.

We compared the histochemical activity of succinate dehydrogenase (SDH) and the intensity of reaction to the presence of myoglobin (Mb) in the same muscle fibres m. longissimus thoracis (MLT), m. semimembranosus (MSM), m. rectus femoris (MRF) and caput longum m. tricipitis brachii (MTB). At the same time we also ascertained the myoglobin content of one gram of fresh muscle. We found that the muscle fibre in all the muscles studied in which there is the most intensive reaction in the test for SDH are also the most intensively coloured in the test for Mb. There is also the same relationship in intermediary and white fibres. The reaction in testing for SDH on a cross section is greatest in the subsarcolemmic area. Similar tendencies appear in the distribution of Mb, although they are less marked and only in fibres with intensive reaction. Different muscles have different myoglobin levels. Muscles with a higher proportion of red and intermediary fibres contain more myoglobin than muscles with a high proportion of white fibres. The quantitative values of the levels of Mb (g/fresh muscle) are not, however, proportional to the share of individual types of muscle fibres in the muscles studied.

[Muscle fibers of pigs sensitive to halothane and resistant to it]. / Svalové vlákna osípaných citlivých na halotan a rezistentných voci nemu.

The histological and histochemical structure of m. longissimus thoracis, m. semimembranosus, m. triceps brachii-caput longum and m. rectus femoris was compared in pigs with a positive and negative reaction to halothane treatment. As found, the pigs affected by the malignant hyperthermia syndrome have thicker muscle fibres in the studied muscles. In the course of growth, the muscles of these animals have a larger proportion of red fibres (SO), but it is already at the age of 170 days (weight 100 kg) that light fibres prevail. The relative volume of the FG fibres is larger by 1-5%, as compared with the healthy animals. A higher number of pathologically changed fibres occurs in the pigs sensitive to halothane. The histological picture is individually variable; therefore the histological and histochemical methods cannot be considered as objective in view of MHS diagnosis. The general weakening of the constitution of the pigs is discussed as a possible predisposition factor underlying the development of various health disorders.