RESUMO
A novel AluI-polymorphism in the fourth intron of chicken growth hormone gene was shown. It was detected the cytosine to thymine transition in the restriction site for AluI. Primers, that flanking the 460 bp fragment of the fourth intron, containing a polymorphic restriction site for AluI, was designed. The nucleotide sequence fragments amplified polymorphic variants was determined. Using designed primers was analyzed the genetic structure of populations of White Plymouth Rock, Poltava Clay, Rhode Island Red and Borkovskaya Barvistaya chicken breeds. It was found that growth hormone gene (by AluI-polymorphism in the fourthintron) was polymorphic in all experimental populations. Frequencies of alleles C and T in chicken population of White Plymouth Rock breed were 0,14 and 0,86; Rhode Island Red 0,3 and 0,7; Poltava Clay 0,04 and 0,96; Borkovskaya Barvistaya 0,08 and 0,92 respectively. The tendency to increase egg production and egg weight of chicken with C/C genotype, as well as meat quality (live weight, carcass weight, weight of pectoral muscles) of chickens with genotype T/T of Rhode Island Red chicken breed was shown.
Assuntos
Galinhas/genética , Desoxirribonucleases de Sítio Específico do Tipo II/química , Hormônio do Crescimento/genética , Íntrons , Polimorfismo de Fragmento de Restrição , Característica Quantitativa Herdável , Alelos , Animais , Sequência de Bases , Cruzamento , Galinhas/anatomia & histologia , Galinhas/classificação , Ovos , Feminino , Expressão Gênica , Frequência do Gene , Genótipo , Masculino , CarneRESUMO
The formation of different types of the artifacts in the amplification reaction with using different classes of the molecular-genetic markers (Indel and SSR) was studied. It was shown that DNA heteroduplexes formed during amplification of heterozygous samples, as fragments of target genes and microsatellite loci. During the electrophoresis in native polyacrylamide gel of the amplification products of homozygous samples for microsatellite loci it was revealed specific additional fragments that do not belong to the class of heteroduplex DNA. It was supposed, that the additional fragments belong to a special type of homoduplex DNA non-linear homoduplexes. The analysis revealed that the formation of a non-linear homoduplex DNA takes place on the 2025 cycle of the PCR, both at the amplification of the individual samples, and individual DNA fragments that was cut out from the gel.
Assuntos
Artefatos , Galinhas/genética , Técnicas de Genotipagem/normas , Repetições de Microssatélites , Reação em Cadeia da Polimerase/normas , Animais , Cruzamento , Galinhas/classificação , Feminino , Marcadores Genéticos , Heterozigoto , Mutação INDEL , Masculino , Eletroforese em Gel de Poliacrilamida Nativa , Ácidos Nucleicos HeteroduplexesRESUMO
The MspI-polymorphism in the fourth intron of the growth hormone gene in populations of White Plymouth Rock, Poltava Clay, Rhode Island Red and Borkovskaya Barvistaya chicken breeds was studied. It is shown that in all examined chicken populations the growth hormone gene is polymorphic. It was found that the presence of "additional" phenotype (restriction pattern) is not associated with duplication of the growth hormone gene. The possibility of formation of heteroduplex DNA of two different types in the course of amplification of heterozygous samples B/C, containing the site 'CCGG', which leads to formation the additional DNA fragment which do not contain the site 'CCGG', was described. The nucleotide sequences of alleles A, B, C and "additional" fragment is described. Frequencies of alleles A, B and C in chicken population of White Plymouth Rock breed were 0.56; 0.16 and 0.28; Poltava Clay--0.10; 0.07 and 0.83; Rhode Island Red--0.27; 0.31 and 0.42; Borkovskaya Barvistaya--0.75; 0.08 and 0.17 respectively.
Assuntos
Galinhas/genética , Desoxirribonuclease HpaII/metabolismo , Hormônio do Crescimento/genética , Íntrons , Polimorfismo de Fragmento de Restrição , Animais , Sequência de Bases , Cruzamento , Eletroforese em Gel de Ágar , Frequência do Gene , Dados de Sequência Molecular , Ácidos Nucleicos Heteroduplexes/genética , Reação em Cadeia da PolimeraseRESUMO
Polymorphism of prolactin and growth hormone genes in two Ukrainian breeding chicken lines of meat-laying (G-2) and eggs performance (A) direction of productivity was studied. Polymorphism of the 24 bp insertion/deletion in the promoter region of prolactin gene, SNP in the -2402 position of the prolactin gene, the first intron of the growth hormone gene was investigated. Frequencies of I and D alleles in line G-2 and line A were 0.135, 0.865 and 0.730, 0.270; frequencies of C and T alleles--0.155, 0.845 and 0.745, 0.255; frequencies of A, B and C alleles--0.435, 0.395, 0.170 and 0.670, 0.160, 0.170 respectively. The results indicate that high frequency of I, C and A alleles in line A corresponds to the egg performance direction.
