[Cellular beta-adrenergic receptor complex in patients with heart failure and its changes during digoxin therapy]. / Beta-adrenoretseptornyi kompleks kletok u bol'nykh s serdechnoi nedostatochnost'iu i ego izmeneniia pri terapii digoksinom.

Twenty eight males with dilated cardiomyopathy, 5 males with coronary heart disease concurrent with postinfarction cardiosclerosis, 4 males and 2 females with rheumatic heart disease were examined. The findings suggest desensitization of the cellular beta-adrenoreceptor complex in patients with circulatory failure, which appeared as lower beta-adrenoreceptor density with cardiac decompensation progression and impaired transmission of a hormonal signal in the cell, particularly in patients with dilated cardiomyopathy. Digoxin therapy let to an increase in beta-adrenoreceptor density with its initial decrease and to adenylate cyclase activity enhancement. The absence of a positive ++clinico-hemodynamic effect and time course of plasma catecholamine levels in some patients with severe circulatory failure during digoxin use, as well as no changes in the values of the beta-adrenoreceptor adenylate cyclase complex indicate that the abnormalities in beta-adrenergic regulation play an important role in the pathogenesis of refractoriness at the cellular level. Retention of forskolin's stimulant effect allows one to expect a significant positive therapeutic response to be shown with the usage of drugs whose load point is a catalytic subunit of adenylate cyclase.

[The platelet factor 4 content of the blood in induced myocardial ischemia in patients with stable stenocardia]. / Soderzhanie 4-go faktora trombotsitov v krovi na fone indutsirovannoi ishemii miokarda u bol'nykh stabil'noi stenokardiei.

Seventeen patients were studied: 10 with stable angina and coronary atherosclerosis, and 7 with "intact" coronary arteries (a control group). All patients underwent coronarography and catheterization of the coronary sinus. Blood samples were obtained from the left ventricle and coronary sinus in 3 points: at rest, peak pacing, 10 min after pacing. All patients with stable angina had positive atrial pacing test (pain and/or ECG deviations and lactate production), in control group similar changes were not observed. However, no statistically significant difference was found in platelet factor (IV) 4 levels between the groups and following pacing; the level of platelet factor IV was near 90 ng/ml. It is concluded that patients with stable angina during pacing-induced ischemia had no significant platelet activation, which may provoke thrombotic events.

[Heterogeneity of brain-specific proteins of S100 type]. / O geterogennosti mozgospetsificheskikh belkov gruppy S100.

A procedure for preparative separation of proteins from bovine brain precipitated in a saturated solution by ammonium sulfate (pH 4.0) and remaining in the supernatant is described. It was shown that not less than 22 fractions (up to 28 fractions considering the "equivocal" reaction) produce different responses to the antiserum against the major protein, i.e. S100.