RESUMO
⢠The effects of free radicals, ·OH and ·NO, generated in polluted dew water on needles of Pinus densiflora (Japanese Red pine) were investigated. ⢠·OH-generating solutions (HOOH with Fe(III) and oxalate ion; ·OH treatment) and ·OH- and ·NO-generating solutions (NO2 - ; ·OH/·NO treatment) were regulated at 25, 50 and 100 µmol and pH 4.4. HOOH only (HOOH treatment) was used as a control solution. Solutions were applied as a mist to the needle surface of P. densiflora seedlings before dawn twice a week for 3 months. ⢠Within a month, net photosynthesis at near saturating irradiance (Pn) and stomatal conductance (gl) of ·OH-treated needles decreased with increasing solution concentration. The HOOH treatment had no effects on any of the measured parameters. Therefore, ·OH in the artificial dews caused the decreases in Pn and gl. In ·OH/·NO-treated needles, gl increased during the experiment, but Pn was unchanged. In all experiments, the characteristics of PSII were not significantly altered. ⢠Free radicals in polluted dew water have harmful effects on the photosynthesis of P. densiflora and compound effects of ·OH and ·NO are different.