Variations in MLL amplification in a patient with acute myeloid leukemia.

In a 66 years old female patient with acute myeloblastic leukemia (AML) complex chromosomal rearrangements involving 11q23 were diagnosed by G-banding and confirmed by different fluorescence in situ hybridization (FISH) techniques. The amplification of MLL gene differed in various sidelines as shown by locus specific probes for 11q23 and 11q13. Complex karyotype rearrangements involving deletions del(5)(q31) and del(7)(q31) were verified by multicolor fluorescence in situ hybridization (mFISH).