Effect of vitamin E deficiency on oxidative metabolism and antioxidant enzyme activity of macrophages.

In AUG rats, deprived of vitamin E for 90 days, we noted a 3-fold increase of kinetic parameters of luminol-dependent chemiluminescence of macrophages, stimulated with opsonized zymosan, superoxide dismutase activity decrease and increment of plasma membrane lipid bilayer microviscosity, which was estimated by fluorescent probe pyrene eximerization method. Vitamin E deficiency did not affect glutathione peroxidase and glutathione reductase activities of macrophages.

[Mathematical modelling of perorally induced immunological tolerance]. / Matematicheskoe modelirovanie peroral'no indutsirovannoi immunologicheskoi tolerantnosti.

A mathematical model of the mechanism of development of orally induced immunologic tolerance has been suggested. The model presents a system of differential non-linear equations, and it is realized as a program in FORTRAN. The model describes primary and secondary immune responses, reflects the main features of the immune system response to antigen intake with food. The immune system model response to varying doses and frequency of the antigen intake with food has been studied. It has been established that repeated administration of small doses of the food antigen leads to a deeper tolerance due to lower stimulation of the immune system. The existence of optimal tolerogenic doses of the food antigen has been proved. Qualitative changes in the immune system response to the food antigen have been recorded in case of increased permeability of the intestinal wall.

[Effect of a phosphatidylcholine-enriched diet on the structural and functional parameters of rat liver microsomes]. / Vliianie diety, obogashchennoi fosfatidilkholinom, na strukturno-funktsional'nye parametry mikrosom pecheni krys.

The authors studied the influence of the diet enriched with phosphatidylcholine on the structural-functional parameters of microsomes: lipid peroxidation activity, the levels of cytochromes b5 and P-450, microviscosity of the lipid phase of the membrane. In experiments in vivo it was found that phosphatidylcholine per se, not combined with antioxidants, did not show antioxidant properties. Biological effects of lecithin could be explained by its role as a catalyst of the monooxygenase system in the liver, based on the correlation (r = +0.8) between the high level of cytochrome b5 and the degree of eximerization of the fluorescent probe pyrene in the microsomes.