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High-quality emission centers in two-dimensional materials are promising components for future photonic and optoelectronic applications. Carbon-enriched hexagonal boron nitride (hBN:C) layers host atom-like color-center (CC) defects with strong and robust photoemission up to room temperature. Placing the hBN:C layers on top of Ag triangle nanoparticles (NPs) accelerates the decay of the CC defects down to 46 ps from their reference bulk value of 350 ps. The ultrafast decay is achieved due to the efficient excitation of the plasmon modes of the Ag NPs by the near field of the CCs. Simulations of the CC/Ag NP interaction show that higher Purcell values are expected, although the measured decay of the CCs is limited by the instrument response. The influence of the NP thickness on the Purcell factor of the CCs is analyzed. The ultrafast operation of the CCs in hBN:C layers paves the way for their use in demanding applications, such as single-photon emitters and quantum devices.
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Plasmon resonances of noble metal nanoparticles are used to enhance light-matter interactions in the nanoworld. The nanoparticles' optical response depends strongly on the dielectric permittivity of the surrounding medium. We show that the plasmon resonance energy of core-shell Au@Mo6 nanoparticles can be tuned from 2.4 to 1.6 eV by varying the thickness of their Mo6 cluster shells between zero and 70 nm, when the core diameter is fixed at 100 nm. We probe their plasmonic response by performing nanometer-resolution plasmon mapping on individual nanoparticles, using electron energy-loss spectroscopy inside a transmission electron microscope. Our experimental results are corroborated by numerical simulations performed using boundary element methods. The simulations predict a similar dependency for the extinction energy, showing that this effect could also be observed by light-optical experiments outside the electron microscope, although limited by the size distribution of the nanoparticles in solution and the substantial scattering effects.
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We measured angle-resolved reflection spectra of triangular-lattice photonic crystal slabs fabricated in a silicon-on-insulator wafer in the mid-infrared region. We achieved a high angle-resolution measurement by means of our homemade optical setup integrated in the sample chamber of an FT-IR spectrometer. By examining the reflection peak frequency as a function of the lateral component of the wave vector of the incident light and applying the selection rules expected from the spatial symmetry of electromagnetic eigenmodes in C6v-symmetric structures, we successfully obtained the dispersion relation and the mode symmetry of the photonic crystal slabs, which agreed well with numerical calculations by the finite element method. We also found the redistribution of diffraction loss between A1- and E1-symmetric modes, which was caused by the Dirac-cone formation due to their effective degeneracy.
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We provide an extensive and systematic investigation of exciton dynamics in droplet epitaxial quantum dots comparing the cases of (311)A, (001), and (111)A surfaces. Despite a similar s-shell exciton structure common to the three cases, the absence of a wetting layer for (311)A and (111)A samples leads to a larger carrier confinement compared to (001), where a wetting layer is present. This leads to a more pronounced dependence of the binding energies of s-shell excitons on the quantum dot size and to the strong anti-binding character of the positive-charged exciton for smaller quantum dots. In-plane geometrical anisotropies of (311)A and (001) quantum dots lead to a large electron-hole fine interaction (fine structure splitting (FSS) â¼100 µeV), whereas for the three-fold symmetric (111)A counterpart, this figure of merit is reduced by about one order of magnitude. In all these cases, we do not observe any size dependence of the fine structure splitting. Heavy-hole/light-hole mixing is present in all the studied cases, leading to a broad spread of linear polarization anisotropy (from 0 up to about 50%) irrespective of surface orientation (symmetry of the confinement), fine structure splitting, and nanostructure size. These results are important for the further development of ideal single and entangled photon sources based on semiconductor quantum dots.
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Fourier transform (FT) spectroscopy is a versatile technique for studying the infrared (IR) optical response of solid-, liquid-, and gas-phase samples. In standard Fourier transform infrared (FT-IR) spectrometers, a light beam passing through a Michelson interferometer is focused onto a sample with condenser optics. This design enables us to examine relatively small samples, but the large solid angle of the focused infrared beam makes it difficult to analyze angle-dependent characteristics. Here, we design and construct a high-precision angle-resolved reflection setup compatible with a commercial FT-IR spectrometer. Our setup converts the focused beam into an achromatically collimated beam with an angle dispersion as high as 0.25°. The setup also permits us to scan the incident angle over â¼8° across zero (normal incidence). The beam diameter can be reduced to â¼1 mm, which is limited by the sensitivity of an HgCdTe detector. The small-footprint apparatus is easily installed in an FT-IR sample compartment. As a demonstration of the capability of our reflection setup, we measure the angle-dependent mid-infrared reflectance of two-dimensional photonic crystal slabs and determine the in-plane dispersion relation in the vicinity of the Γ point in momentum space. We observe the formation of photonic Dirac cones, i.e., linear dispersions with an accidental degeneracy at Γ, in an ideally designed sample. Our apparatus is useful for characterizing various systems that have a strong in-plane anisotropy, including photonic crystal waveguides, plasmonic metasurfaces, and molecular crystalline films.
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Droplet epitaxy allows the efficient fabrication of a plethora of 3D, III-V-based nanostructures on different crystalline orientations. Quantum dots grown on a (311)A-oriented surface are obtained with record surface density, with or without a wetting layer. These are appealing features for quantum dot lasing, thanks to the large density of quantum emitters and a truly 3D lateral confinement. However, the intimate photophysics of this class of nanostructures has not yet been investigated. Here, we address the main optical and electronic properties of s-shell excitons in individual quantum dots grown on (311)A substrates with photoluminescence spectroscopy experiments. We show the presence of neutral exciton and biexciton as well as positive and negative charged excitons. We investigate the origins of spectral broadening, identifying them in spectral diffusion at low temperature and phonon interaction at higher temperature, the presence of fine interactions between electron and hole spin, and a relevant heavy-hole/light-hole mixing. We interpret the level filling with a simple Poissonian model reproducing the power excitation dependence of the s-shell excitons. These results are relevant for the further improvement of this class of quantum emitters and their exploitation as single-photon sources for low-density samples as well as for efficient lasers for high-density samples.
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The dispersion relation and the angle-resolved reflection spectra of triangular-lattice photonic crystal slabs of the C6v symmetry were examined by the finite element method. The Dirac-cone dispersion relation on the Γ point of the reciprocal space was confirmed. The reflection spectra showed unique selection rules that agreed with the analytical calculation by the k · p perturbation theory. The distortion of the liner dispersion relation of the Dirac cones due to diffraction loss was also reproduced well by the numerical calculation, while we found distortion-free Dirac cones materialized with E2-symmetric modes.
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We materialized the isotropic Dirac-cone dispersion relation in the mid-infrared range by fabricating photonic crystal slabs of the C4v symmetry in SOI (silicon-on-insulator) wafers by electron beam lithography. The dispersion relation was examined by the angle-resolved reflection spectra with our home-made high-resolution apparatus, which showed a good agreement with the dispersion relation and the reflection spectra calculated by the finite element method. The reflection spectra also agreed with the selection rules derived from the spatial symmetry of the Dirac-cone modes, which proved to be a powerful tool for the mode assignment.
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The purpose of this study was to clarify the roles of ERM proteins (ezrin/radixin/moesin) in the regulation of membrane localization and transport activity of transporters at the human blood-brain barrier (BBB). Ezrin or moesin knockdown in a human in vitro BBB model cell line (hCMEC/D3) reduced both BCRP and GLUT1 protein expression levels on the plasma membrane. Radixin knockdown reduced not only BCRP and GLUT1, but also P-gp membrane expression. These results indicate that P-gp, BCRP and GLUT1 proteins are maintained on the plasma membrane via different ERM proteins. Furthermore, moesin knockdown caused the largest decrease of P-gp and BCRP efflux activity among the ERM proteins, whereas GLUT1 influx activity was similarly reduced by knockdown of each ERM protein. To investigate how moesin knockdown reduced P-gp efflux activity without loss of P-gp from the plasma membrane, we examined the role of PKCßI. PKCßI increased P-gp phosphorylation and reduced P-gp efflux activity. Radixin and moesin proteins were detected in isolated human brain capillaries, and their protein abundances were within a 3-fold range, compared with those in hCMEC/D3 cell line. These findings may mean that ezrin, radixin and moesin maintain the functions of different transporters in different ways at the human BBB.
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Barreira Hematoencefálica/metabolismo , Membrana Celular/metabolismo , Proteínas do Citoesqueleto/metabolismo , Células Endoteliais/metabolismo , Proteínas de Membrana/metabolismo , Proteínas de Membrana Transportadoras/metabolismo , Proteínas dos Microfilamentos/metabolismo , Idoso , Linhagem Celular , Proteínas do Citoesqueleto/genética , Técnicas de Silenciamento de Genes , Humanos , Masculino , Proteínas de Membrana/genética , Proteínas dos Microfilamentos/genética , Microvasos/metabolismo , RNA Interferente Pequeno/genética , TransfecçãoRESUMO
The long dreamed 'quantum internet' would consist of a network of quantum nodes (solid-state or atomic systems) linked by flying qubits, naturally based on photons, travelling over long distances at the speed of light, with negligible decoherence. A key component is a light source, able to provide single or entangled photon pairs. Among the different platforms, semiconductor quantum dots (QDs) are very attractive, as they can be integrated with other photonic and electronic components in miniaturized chips. In the early 1990s two approaches were developed to synthetize self-assembled epitaxial semiconductor QDs, or 'artificial atoms'-namely, the Stranski-Krastanov (SK) and the droplet epitaxy (DE) methods. Because of its robustness and simplicity, the SK method became the workhorse to achieve several breakthroughs in both fundamental and technological areas. The need for specific emission wavelengths or structural and optical properties has nevertheless motivated further research on the DE method and its more recent development, local droplet etching (LDE), as complementary routes to obtain high-quality semiconductor nanostructures. The recent reports on the generation of highly entangled photon pairs, combined with good photon indistinguishability, suggest that DE and LDE QDs may complement (and sometimes even outperform) conventional SK InGaAs QDs as quantum emitters. We present here a critical survey of the state of the art of DE and LDE, highlighting the advantages and weaknesses, the achievements and challenges that are still open, in view of applications in quantum communication and technology.
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A hybrid metal-semiconductor nanosystem for the generation of THz radiation, based on the fabrication of GaAs quantum molecules-Ga metal nanoparticles complexes through a self assembly approach, is proposed. The role of the growth parameters, the substrate temperature, the Ga and As flux during the quantum dot molecule (QDM) fabrication and the metal nanoparticle alignment are discussed. The tuning of the relative positioning of QDMs and metal nanoparticles is obtained through the careful control of Ga droplet nucleation sites via Ga surface diffusion. The electronic structure of a typical QDM was evaluated on the base of the morphological characterizations performed by atomic force microscopy and cross sectional scanning electron microscopy, and the predicted results confirmed by micro-photoluminescence experiments, showing that the Ga metal nanoparticle-GaAs quantum molecule complexes are suitable for terahertz generation from intraband transition.
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We show by the first-order perturbation theory and the configuration interaction method that the Coulomb interaction in quantum rings mixes electron-hole pair states with the same total angular momentum, which makes it difficult to observe a clear excitonic Aharonov-Bohm (A-B) effect. To avoid this situation, we propose the use of a combined structure of a quantum dot on the top of a quantum ring with an applied static electric field. Under moderate experimental conditions with respect to the applied electric and magnetic fields, we show that we can observe the excitonic A-B effect due to the reduction of the Coulomb interaction and an increase in the difference between the average radii of the electron and hole trajectories.
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A warning signal presented via a visual or an auditory cue might interfere with auditory or visual information inside and outside a vehicle. On the other hand, such interference would be certainly reduced if a tactile cue is used. Therefore, it is expected that tactile cues would be promising as warning signals, especially in a noisy environment. In order to determine the most suitable modality of cue (warning) to a visual hazard in noisy environments, auditory and tactile cues were examined in this study. The condition of stimulus onset asynchrony (SOA) was set to 0ms, 500ms, and 1000ms. Two types of noises were used: white noise and noise outside a vehicle recorded in a real-world driving environment. The noise level LAeq (equivalent continuous A-weighted sound pressure level) inside the experimental chamber of each type of noise was adjusted to approximately 60 dB (A), 70 dB (A), and 80 dB (A). As a result, it was verified that tactile warning was more effective than auditory warning. When the noise outside a vehicle from a real-driving environment was used as the noise inside the experimental chamber, the reaction time to the auditory warning was not affected by the noise level.
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Percepção Auditiva , Sinais (Psicologia) , Detecção de Sinal Psicológico , Percepção do Tato , Estimulação Acústica , Condução de Veículo , Simulação por Computador , Humanos , Masculino , Ruído , Estimulação Física , Tempo de Reação , Análise e Desempenho de Tarefas , Vibração , Percepção Visual , Adulto JovemRESUMO
Type-2 diabetes mellitus (T2DM) induces bone frailty. Protein and polyunsaturated fatty acids (PUFA) contained in fish can be effective in enhancing bone quality, but the bone developing effect of fish protein containing less PUFA has not been evaluated in young animals with T2DM. We prepared a bonito fish (BF) and defatted BF (DBF) and hypothesized that protein contained in BF and DBF would be effective for mitigating the effects of T2DM-induced bone frailty. We mainly evaluated the effect of dietary BF and DBF on bone and apparent calcium absorption in young Goto-Kakizaki (GK) rats with T2DM. GK rats were divided into 3 groups based on diets (casein, BF, and DBF) and fed with each diet for 6 wk. Wistar rats were fed with the casein diet as a non-T2DM control. Bone mass, bone strength, apparent calcium absorption, and serum biochemical parameters were determined. The dry weight and strength of the femurs were lower in the GK rats than in the Wistar rats fed with the casein diet. Dietary intake of the BF and DBF diets enhanced the maximum load and dry weight of the femurs and suppressed the serum alkaline phosphatase activity although the apparent calcium absorption was lower in the GK rats fed with the BF and DBF diets than in those fed with the casein diet. These parameters were not different between the rats fed with the BF and DBF diets. Our data suggest that protein contained in the BF and DBF diets improved T2DM-induced bone frailty.
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Doenças Ósseas Metabólicas/prevenção & controle , Diabetes Mellitus Tipo 2/complicações , Dieta , Proteínas Alimentares/farmacologia , Fêmur/efeitos dos fármacos , Peixes , Alimentos Marinhos , Fosfatase Alcalina/sangue , Animais , Densidade Óssea , Doenças Ósseas Metabólicas/etiologia , Cálcio/metabolismo , Diabetes Mellitus Tipo 2/sangue , Proteínas Alimentares/uso terapêutico , Fêmur/metabolismo , Masculino , Ratos , Ratos WistarRESUMO
We investigated the fat metabolic characteristics in non-obese and diabetic Goto-Kakizaki (GK) rat and the effects of dietary egg white hydrolysate (EWH) on glucose and fat metabolism. Wistar (W) and GK (G) rats were placed into dietary casein (WC and GC) or EWH (WE and GE) group, and fed their respective diet for six weeks. Triglyceride (TG) content and stearoyl-CoA desaturase (SCD) indices in the soleus muscle were higher in the GC group than WC group in parallel with worsening serum glucose metabolic parameters. The glucose metabolic parameters were significantly improved in the GE group. The TG accumulation and SCD indices in the soleus muscle were also significantly lower in the GE group than in the GC group. In conclusion, dietary EWH not only improved glucose metabolism but also reduced both TG accumulation and SCD indices in the soleus muscle of GK rat.
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Diabetes Mellitus Tipo 2/dietoterapia , Proteínas Dietéticas do Ovo/uso terapêutico , Hiperglicemia/prevenção & controle , Hipoglicemiantes/uso terapêutico , Músculo Esquelético/metabolismo , Hidrolisados de Proteína/uso terapêutico , Triglicerídeos/metabolismo , Animais , Glicemia/análise , Diabetes Mellitus Tipo 2/sangue , Diabetes Mellitus Tipo 2/metabolismo , Diabetes Mellitus Tipo 2/fisiopatologia , Regulação para Baixo , Proteínas Dietéticas do Ovo/química , Clara de Ovo/química , Hiperglicemia/etiologia , Hipertrigliceridemia/etiologia , Hipertrigliceridemia/prevenção & controle , Hipoglicemiantes/química , Hipolipemiantes/química , Hipolipemiantes/uso terapêutico , Resistência à Insulina , Masculino , Músculo Esquelético/enzimologia , Ratos Endogâmicos , Ratos Wistar , Estearoil-CoA Dessaturase/metabolismo , Triglicerídeos/sangueRESUMO
Galactocerebroside (Gal-C) is a major myelin component in the central nervous system. The anti-Gal-C antibody induced by mycoplasma infection may therefore be involved in the pathogenic mechanisms of mycoplasma-associated encephalitis. Here we report an adult case of mycoplasma encephalitis developing excessive daytime sleepiness. Brain MRI suggested that hypothalamic involvement was compatible with hypersomnia. This finding was corroborated by decreased hypocretin-1 in cerebrospinal fluid (CSF) and the manifestation of diabetes insipidus. Screening for anti-glycolipid antibody profiles showed the selective increase of serum anti-Gal-C antibody. After treatment with minocyclin, the patient's daytime sleepiness was markedly improved and the CSF hypocretin-1 level became almost normal, as well. It is known that CSF hypocretin-1 is decreased in Guillain-Barré syndrome mediated by anti-glycolipid antibody, suggesting a possible mechanistic link between anti-glycolipid antibodies and hypothalamic involvement. The present case further emphasizes the broad spectrum of neurological complications after mycoplasma infection.
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Distúrbios do Sono por Sonolência Excessiva/sangue , Galactosilceramidas/sangue , Meningoencefalite/sangue , Mycoplasma pneumoniae , Pneumonia por Mycoplasma/sangue , Adulto , Autoanticorpos/sangue , Diagnóstico Diferencial , Distúrbios do Sono por Sonolência Excessiva/diagnóstico , Distúrbios do Sono por Sonolência Excessiva/etiologia , Humanos , Masculino , Meningoencefalite/diagnóstico , Meningoencefalite/etiologia , Pneumonia por Mycoplasma/complicações , Pneumonia por Mycoplasma/diagnósticoRESUMO
We report on the implementation of a scanning Fabry-Pérot interferometer for photoluminescence spectroscopy investigation. We choose a conveniently small reflectivity of the two planar semitransparent mirrors which, in spite of a moderate cavity finesse, ensures a good mechanical stability over a long time. We also exploit the large tuneability of the cavity length (i.e., of the free spectral range) for changing the spectral resolution over two order of magnitude (from ~300 µeV to ~4 µeV in full width at half maximum). Such a characteristic easily allows to scan both sharp and broad luminescence bands. We test our Fabry-Pérot interferometer on sharp photoluminescence lines resulting from excitonic recombination in self-assembled GaAs quantum dots. We demonstrate the ability of our system to resolve linewidth as small as 4 µeV.
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The use of an inferolateral extension technique of a groin flap has previously been reported. This technique involves harvesting an extended portion from the anterolateral thigh, including the lateral femoral cutaneous nerve (LFCN) and its accompanying vessels, attached to a groin flap via communications between the LFCN-accompanying vessels and the superficial circumflex iliac artery (SCIA) system. In this study, we used this technique involving a vascularized LFCN combined with a groin flap to reconstruct a facial nerve defect. The patient was a 58-year-old man with a salivary duct carcinoma in the left parotid gland. Tumor ablation resulted in a defect of the skin and soft tissue including all branches of the facial nerve. A free groin flap was harvested based on the SCIA system, composed of the LFCN and a small monitoring flap, which were nourished by the LFCN-accompanying vessels and by communication with the SCIA system. The LFCN was transplanted into the gaps in the facial nerve branches as a cable graft, and the skin flap was used to cover and fill the soft tissue defect. The postoperative course was uneventful and satisfactory facial animation was obtained. This represents a possible technique for nerve reconstruction using a vascularized nerve graft.
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Nervo Facial/cirurgia , Invasividade Neoplásica/patologia , Procedimentos de Cirurgia Plástica/métodos , Retalhos Cirúrgicos/irrigação sanguínea , Coxa da Perna/irrigação sanguínea , Carcinoma Ductal/patologia , Carcinoma Ductal/cirurgia , Estética , Nervo Femoral/cirurgia , Nervo Femoral/transplante , Sobrevivência de Enxerto , Humanos , Artéria Ilíaca/cirurgia , Artéria Ilíaca/transplante , Masculino , Pessoa de Meia-Idade , Esvaziamento Cervical/métodos , Neoplasias Parotídeas/patologia , Neoplasias Parotídeas/cirurgia , Medição de Risco , Coxa da Perna/cirurgia , Resultado do Tratamento , Cicatrização/fisiologiaRESUMO
Inhibition of angiopoietin-2 (Ang2) can slow tumor growth, but the underlying mechanism is not fully understood. Because Ang2 is expressed in growing blood vessels and promotes angiogenesis driven by vascular endothelial growth factor (VEGF), we asked whether the antitumor effect of Ang2 inhibition results from reduced sprouting angiogenesis and whether the effect is augmented by inhibition of VEGF from tumor cells. Using Colo205 human colon carcinomas in nude mice as a model, we found that selective inhibition of Ang2 by the peptide-Fc fusion protein L1-7(N) reduced the number of vascular sprouts by 46% and tumor growth by 62% over 26 days. Strikingly, when the Ang2 inhibitor was combined with a function-blocking anti-VEGF antibody, the number of sprouts was reduced by 82%, tumor vascularity was reduced by 67%, and tumor growth slowed by 91% compared with controls. The reduction in tumor growth was accompanied by decreased cell proliferation and increased apoptosis. We conclude that inhibition of Ang2 slows tumor growth by limiting the expansion of the tumor vasculature by sprouting angiogenesis, in a manner that is complemented by concurrent inhibition of VEGF and leads to reduced proliferation and increased apoptosis of tumor cells.
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Inibidores da Angiogênese/farmacologia , Angiopoietina-2/antagonistas & inibidores , Protocolos de Quimioterapia Combinada Antineoplásica/farmacologia , Neoplasias do Colo/irrigação sanguínea , Neoplasias do Colo/tratamento farmacológico , Fator A de Crescimento do Endotélio Vascular/antagonistas & inibidores , Angiopoietina-2/biossíntese , Animais , Anticorpos Monoclonais/administração & dosagem , Anticorpos Monoclonais/imunologia , Anticorpos Monoclonais/farmacologia , Processos de Crescimento Celular/efeitos dos fármacos , Neoplasias do Colo/patologia , Sinergismo Farmacológico , Humanos , Camundongos , Camundongos Nus , Neovascularização Patológica/tratamento farmacológico , Neovascularização Patológica/patologia , Ratos , Receptores Fc/genética , Proteínas Recombinantes de Fusão/administração & dosagem , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/farmacologia , Fator A de Crescimento do Endotélio Vascular/biossíntese , Fator A de Crescimento do Endotélio Vascular/imunologia , Ensaios Antitumorais Modelo de XenoenxertoRESUMO
Coherent anti-Stokes Raman scattering (CARS) associated with the second-order Raman process has been confirmed in diamond. Frequency dependence of CARS was analyzed using two-color synchronously-generated picosecond pulses. Resonance enhancement of chi((3)) was observed at an energy shift corresponding to the overtone Sigma branch (K point) phonon, as well as the fundamental Raman-active Gamma((25+)) phonon. The excitation spectrum of CARS at the two-phonon resonance region showed dispersive dependence, reflecting the interference of resonant CARS and nonresonant four-wave mixing scattering.