Antiviral activity of liposomal preparations of antibiotic geliomycin.

A liposomal preparation with maximally possible content of incorporated geliomycin was obtained. Its cytotoxicity was studied in a culture of human embryonal diploid fibroblasts. Antiviral activity was studied on a model of cytomegaloviral infection in vitro by the capacity to plaque formation. Liposomal geliomycin was 10-fold less toxic for human cells than the solution of the antibiotic in DMSO and exhibited antiviral activity towards cytomegaloviral infection at a concentration of 0.042 microg/ml.

[Characteristics of the regeneration process in the liver deprived of polyploid hepatocytes]. / Osobennosti regeneratsionnogo protsessa v pecheni, ne soderzhashchei poliploidnykh gepatotsitov.

By applying autoradiography with 3H-thymidine hepatocytes of young adult guinea pigs were found to enter the DNA-synthesis phase 25 hours after partial hepatectomy. Peaks of nuclei labeling were revealed 30, 45, and 60 hours after the operation. By calculating mitotic figures on squash preparations two waves of mitosis were found (in 40 and 55 hours) after hepatectomy. Cytophotometric study of DNA content showed practically all mononuclear and binuclear hepatocytes to contain diploid nuclei in 3 and 5 days after the operation. By the end of the 7th regeneration day there were revealed 6% mononuclear tetraploid cells. The percentage of binuclear cells during the period of regeneration under study decreased from 16 to 8%. A conclusion was drawn that the main cytologicalmechanism of the guinea pig liver regeneration was full-fledged mitosis ending by cell division.