Assuntos
Proteoglicanas de Sulfatos de Condroitina/fisiologia , Ácido Hialurônico/fisiologia , Sulfato de Queratano/fisiologia , Proteínas/fisiologia , Proteoglicanas/metabolismo , Animais , Biopolímeros , Agregação Eritrocítica , Eritrócitos/citologia , Eritrócitos/metabolismo , Lumicana , Proteoglicanas/química , Coelhos , Espectrofotometria InfravermelhoRESUMO
Aggregation of erythrocytes in the blood of rabbits was shown to depend on concentration of proteoglycans in the blood plasma. In experimental sepsis (Str. haemolyticus) there occurred simultaneous increase of concentration of proteoglycans in plasma and increased aggregation of erythrocytes in the blood. In pregnancy, the concentration of proteoglycans in the blood was constant at a low level throughout the period of pregnancy.
Assuntos
Carcinoma de Brown-Pearce/sangue , Agregação Eritrocítica , Prenhez/sangue , Infecções Estreptocócicas/sangue , Streptococcus pyogenes , Animais , Biopolímeros , Feminino , Hexosaminas/sangue , Masculino , Transplante de Neoplasias , Gravidez , Coelhos , Fatores de TempoRESUMO
Rabbit and mice were injected into the blood stream sodium hyaluronate (0.1 mg per 1 g of the body animal) and protein-chondroitin-keratan-sulfate sodium (0.2 mg per 1 g of the body animal) in 0.15 M NaCl solution. It was shown that both proteoglycans in the blood stream the aggregation action on the erythrocytes in the blood stream. The action finished after 24 hours later on the injection of proteoglycans during in which time the circulating the proteoglycans is remove out of the plasma.
Assuntos
Eritrócitos/efeitos dos fármacos , Proteoglicanas/farmacologia , Animais , Biopolímeros , Circulação Sanguínea/efeitos dos fármacos , Proteoglicanas de Sulfatos de Condroitina/farmacologia , Agregação Eritrocítica/efeitos dos fármacos , Ácido Hialurônico/farmacologia , Sulfato de Queratano/farmacologia , Lumicana , Camundongos , CoelhosRESUMO
The effect of amount of rabbit erythrocytes and concentration of sodium hyaluronate and sodium salt of protein--chondroitin-keratan-sulfate were studied on aggregation of erythrocytes suspended in 0.15 M NaCL, pH 7.4. It was shown that the rate of steric exclusion of erythrocytes depends on relationship between amount of erythrocytes and concentrations of these proteoglycans.
Assuntos
Eritrócitos/efeitos dos fármacos , Proteoglicanas/farmacologia , Animais , Proteoglicanas de Sulfatos de Condroitina/farmacologia , Relação Dose-Resposta a Droga , Agregação Eritrocítica/efeitos dos fármacos , Contagem de Eritrócitos/efeitos dos fármacos , Eritrócitos/química , Ácido Hialurônico/farmacologia , Sulfato de Queratano/farmacologia , Lumicana , Coelhos , SoluçõesRESUMO
The infrared spectra of the hyalin cartilage vitreous body, cornea, sclera and spectra of standard normal Na(+)-salts of hyaluronic acid, chondroitin-4-sulfate, chondroitin-6-sulfate, protein-chondroitin-keratan-sulfate, aggregates of proteoglycans of the hyalin cartilage, heparin fractions containing 3 and 4 residues of sulfuric acid per dimer of polymer were obtained. The comparative analysis of the spectra for tissues and of the spectra for proteoglycans made it possible to identify different proteoglycans in the same tissues.
Assuntos
Proteoglicanas/análise , Espectrofotometria Infravermelho , Animais , Cartilagem/química , Bovinos , Sulfatos de Condroitina/análise , Córnea/química , Humanos , Ácido Hialurônico/análise , Sulfato de Queratano/análise , Esclera/química , Corpo Vítreo/químicaRESUMO
The IR spectra of sodium salt hyaluronic acid, chondroitin-4-sulfate, chondroitin-6-sulfate, dermatan-sulfate, protein-chondroitin-keratan-sulfate and aggregates of proteoglycans of the hyaline cartilage, heparin fractions containing 3 and 4 residues of sulfuric acid per dimer of polymer were obtained. It was shown that comparative analysis of IR spectra of the proteoglycans makes it possible to identify the 1150 cm-1, 1125 cm-1.
Assuntos
Proteoglicanas/análise , Heparina/análise , Espectrofotometria Infravermelho/métodos , Relação Estrutura-AtividadeRESUMO
The infrared spectra of standard hyaluronic acid, proteochondroitin sulfate, aggregates of proteoglycans and spectra of the vitreous body, cornea and sclera were obtained in the region of 4000-400 cm-1. The comparative analysis of the spectra of these tissues and spectra made it possible to identify greater absorption bands of these biopolymers.
Assuntos
Olho/química , Proteoglicanas/análise , Espectrofotometria Infravermelho , Animais , Proteoglicanas de Sulfatos de Condroitina/análise , Córnea/química , Cristalino/química , Coelhos , Esclera/química , Corpo Vítreo/químicaRESUMO
Chemically isolated preparations of acid and normal Mg2+, Ca2+-, and guanidine+ salts of cartilage proteoglycan aggregates were investigated by electron microscopy. Some differences between acid and normal salts depend on the amount, valency and distribution of basic ion as well as between carboxyl and sulphate groups.
Assuntos
Proteoglicanas/ultraestrutura , Adulto , Animais , Cartilagem/metabolismo , Cartilagem/ultraestrutura , Bovinos , Histocitoquímica , Humanos , Concentração de Íons de Hidrogênio , Masculino , Microscopia Eletrônica , Pessoa de Meia-Idade , Proteoglicanas/metabolismo , Traqueia/metabolismo , Traqueia/ultraestruturaRESUMO
It has been shown that the capacity of Ca2+ salts of hyaluronic acid (HA) and nonaggregating protein-chondroitin-keratan-sulfate (PCKS) to divide in erythrocyte-saline suspension into liquid and cell phases was stronger than the analogous capacity of K+ salts. It was suggested that this is connected with a tendency to form different three-dimensional structures in solutions, which was more expressed in HA and PCKS Ca2+ salts than in K+ salts of these proteoglycans.
Assuntos
Proteoglicanas/farmacologia , Animais , Cálcio/farmacologia , Proteoglicanas de Sulfatos de Condroitina/farmacologia , Relação Dose-Resposta a Droga , Eritrócitos/efeitos dos fármacos , Ácido Hialurônico/farmacologia , Sulfato de Queratano/farmacologia , Lumicana , Conformação Molecular , Potássio/farmacologia , Coelhos , Soluções , SuspensõesRESUMO
The infrared spectra of normal knee joint cartilage, normal and rheumatoid arthritis-affected human synovial membrane and the same normal bovine tissues were obtained over the region of 400--4000 cm-1. A comparative analysis of the spectra of these tissues and those containing hyaluronate, protein-chondroitin-keratan sulfate aggregates of cartilage proteoglycans and heparin made it possible to identify greater absorption bands of these biopolymers in the tissue spectra. The interpretation of the results obtained is presented.
Assuntos
Cartilagem Articular/análise , Membrana Sinovial/análise , Adulto , Animais , Artrite Reumatoide/metabolismo , Bovinos , Humanos , Técnicas In Vitro , Articulação do Joelho , Pessoa de Meia-Idade , Espectrofotometria InfravermelhoRESUMO
The effect of two heparin fractions containing 3 (HP-3) and 4 (HP-4) residues of sulfuric acid per dimer of polymers on the capacity of hyaluronate potassium (HUP) and protein-chondroitin-keratan-sulfate potassium (PCHKSP) to aggregate rabbit erythrocytes suspended in 0.15 M NaCl was studied. HP-3 (0.3-5.0 mg X ml-1) and HP-4 (0.3-5.0 mg X ml-1) was inhibited the aggregating action on the erythrocytes of HUP. Fraction HP-3 (0.3-5.0 mg X ml-1) was activated the aggregating action on the erythrocytes of PCHKSP. Fraction HP-4 when the concentration of their biopolymer were 0.3 mg X ml-1 so activated the aggregating action of PCHKSP, but when the concentration HP-4 0.6-5.0 mg X ml-1 was inhibited the aggregating action PCHKSP. The mixture of HP-3 (1.2 mg X ml-1) and HP-4 (1.2 mg X ml-1) was not influenced on aggregating action of PCHKSP.
Assuntos
Agregação Eritrocítica/efeitos dos fármacos , Heparina/farmacologia , Animais , Proteoglicanas de Sulfatos de Condroitina/farmacologia , Relação Dose-Resposta a Droga , Interações Medicamentosas , Ácido Hialurônico/farmacologia , Técnicas In Vitro , Sulfato de Queratano/farmacologia , Lumicana , Coelhos , SoluçõesRESUMO
The method has been developed for preparing complexes of lysozyme with chondroitin sulfate-4 and -6, non-aggregated (soluble) proteoglycans, cartilage proteoglycan aggregates, heparin fractions containing residues 3(H-3) and 4(H-4) of sulfuric acid per dimer of polymer. The studies of the chemical composition and IK-spectra of proteoglycan-lysozyme complexes have demonstrated the electrostatic nature of proteoglycan-lysozyme interaction.
Assuntos
Proteínas , Proteoglicanas , Fenômenos Químicos , Química , Técnicas In Vitro , Substâncias Macromoleculares , Muramidase , Espectrofotometria InfravermelhoRESUMO
The most important studies on hyaluronic acid carried out within the recent 5-7 years are reviewed. Chemical structure, physical parameters and properties of hyaluronic acid are considered. Various functions of hyaluronic acid are discussed on the basis of current information.
Assuntos
Ácido Hialurônico/fisiologia , Animais , Configuração de Carboidratos , Cartilagem/análise , Adesão Celular , Células Cultivadas , Agregação Eritrocítica , Humanos , Ácido Hialurônico/isolamento & purificação , Ácido Hialurônico/metabolismo , Substâncias Macromoleculares , Conformação Proteica , Proteoglicanas/metabolismoRESUMO
It has been shown that rabbit red cells treated with formalin form aggregates in the presence of hyaluronic acid (HUA) soluble protein-chondroitin-keratan sulfate (PCKS) and cartilage proteoglycan aggregates (PA) but to a lesser degree than normal red cells. It is suggested that the proteoglycans under consideration can specifically interact with red cells. Aggregation of red cells in the presence of HUA, PCKS and PA is the result of the combined action of these two factors.
Assuntos
Agregação Eritrocítica/efeitos dos fármacos , Eritrócitos/efeitos dos fármacos , Proteoglicanas/farmacologia , Animais , Adesão Celular/efeitos dos fármacos , Proteoglicanas de Sulfatos de Condroitina/farmacologia , Formaldeído/farmacologia , Ácido Hialurônico/farmacologia , Sulfato de Queratano/farmacologia , Lumicana , Coelhos , SuspensõesRESUMO
The methods for preparing acid and neutral Na+, K+, Ca2+ and Mg2+ salts of individual heparin fractions were devised. One of the fractions contains 3 (HP = 3) and another 4 (HP = 4) sulfate groups per dimer of the macromolecule of both the polymers. Infrared spectra of the salts were obtained. It was shown that acid salts of HP = 3 (with the exception of Mg2+ salt) and HP = 4 form intermolecular hydrogen bonds. The structure of the infrared spectra of HP = 3 and HP = 4 salts depends, to a significant degree, on the cation nature.
Assuntos
Heparina , Fenômenos Químicos , Química , Concentração de Íons de Hidrogênio , Espectrofotometria InfravermelhoRESUMO
The authors have devised the methods for preparing free hyaluronic acid (HA) and non-aggregating fraction of protein-chondroitin-keratan sulfate (PCKS), as well as those for preparing their Na+, K+, Ca2+ and Mg2+ salts (acid and neutral). Infrared spectroscopy has demonstrated the presence of intermolecular hydrogen bonds, formed by hydroxyl groups, in HA and PCKS macrocomplexes and in PCKS acid salts. HA salts appeared not to form macrocomplexes at the expense of intermolecular hydrogen bonds.