[The level of matrix metalloproteinases and type IV collagen in the gingival mucosa under different clinical forms of periodontitis in pre-and pubertal periods and their prognostic value].

The purpose of the study - the analysis of the prevalence and gravity of periodontal diseases among school adolescents and detection the relationship between the degree of periodontal tissues changes and the hormonal status of the prepubertal and pubertal periods. The total number of observations - 618 Tbilisi schoolchildren at the age of 9 to 17 years. Cohort group (n=50) was divided by age: 1) 9-13 years (n=26), 2) 14-17 years (n=24). The material for morphological and immunohistochemical study were the samples of periodontal tissues, received during the tooth extraction according the orthodontic testimony. The samples of the gingival mucosa was stained by hematoxylin and eosin, in the same samples the Matrix Metalloproteinases expression and activity were determined by immunohistochemistry using a primary antibody MMP-1 and MMP-2 ("Novocastra", UK). The results of immunohistochemical reactions were estimated in scores by semiquantitative method. For the detection of type IV collagen in basement membranes the samples were stained by the "Direct Red-80" ("Sigma"). It was found that the increase of Matrix Metalloproteinases expression and the type IV collagen degeneration in basement membrane depend on the age, particularly the expression of MMP-1 and MMP-2 in the period of 14-17 years (puberty) is upwards than in the group of 9-13 years. Studies have also demonstrated the clinical significance of Matrix Metalloproteinases ММР-1, 2 activity indicating the dissociation of the extracellular substance and type IV collagen in the assessment of severity and prognosis of periodontal complex pathology in pre-and pubertal periods.

[Features of immunohistochemical markers expression in cervical epithelium under thyroid dysfunction].

The purpose of the investigation was to study the morphological variants and molecular markers features of the epithelial component of cervical mucosa under thyroid dysfunction. The study groups were: group A: 180 patients operated by colloidal goiter with verified hypothyreosis treated by L-thyroxin; group B: 168 patients with similar diagnosis without replacement therapy during last 3 years; control group:150 patients with euthyreosis. Mono- and polyclonal antibodies to TSH, EGFR and VEGF were used as primary antibodies. The pathological foci in cervical mucosa displayed the following types of epithelial changes: the epithelium in proliferation stage; epithelial hyperplasia without atypia; atrophic epithelium.; The data were shown an increased expression of TSH, EGFR and VEGF which increased from proliferation to hyperplasia without atypia, also adenomyosis foci was more frequent in group without replacement therapy by L-thyroxin (group B), than in group A with hypothyreosis treated by hormone and in control group - euthyreosis. The foci of AM were characterized by the molecular atypia of TSH and growth factors EGFR and VEGF which indicate on the biological progression of cervical mucosa changes under hypothyreosis.

[The placental histo- and ultrastructure peculiarities during the cytomegalovirus infection in pregnant].

Object of the investigation - estimation of changes of the feto-placental complex and syncytiotrophoblaston based on the placental morphology and the activity of the antiviral antibodies in expectant mothers. The work is based on findings from 50 expectant mothers with cytomegalovirus (CMV) infection - 43 with reactivation of the latent infection according to the data of high avid IgG (86%) and 7 with primary active CMV infection according the level of the low avid IgG (14%). 20 healthy expectant mothers were included in the control group. It was shown that the active primary and reactivated CMV infection accompanied by the drawing in pathologic process the chorion structures in a productive inflammation - chorionamnionis and choriovillusitis manner, which testify to drawing the fetus immune system in reciprocal reaction with subsequent intrauterine growth retardation.