Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros











Base de dados
Intervalo de ano de publicação
1.
J Biophotonics ; 4(3): 193-205, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20740520

RESUMO

In this paper we introduce a custom scanning near-field optical microscope (SNOM) that simultaneously collects reflection and transmission near-field images along with topography. This dual-optical SNOM uses a bent probe, which allows for axial reflection imaging, accurate surface scanning, and easy identification of topographic artifacts. Using this novel dual-optical SNOM, we image desiccated and non-desiccated human breast epithelial tissue. By comparing the simultaneous SNOM images, we isolate the effects of tissue morphology and variations in refractive indices on the forward- and back-scattering of light from the tissue. We find that the reduction in back-scattering from tissue, relative to the glass slide, is caused by dense packing of the scattering sites in the cytoplasm (morphology) in the desiccated tissue and a thin-film of water adhering to the glass slide (refractive index) in the non-desiccated tissue sample. Our work demonstrates the potential of our customized dual-optical SNOM system for label-free tissue diagnostics.


Assuntos
Mama/patologia , Epitélio/patologia , Aumento da Imagem/métodos , Microscopia de Varredura por Sonda/métodos , Espalhamento de Radiação , Mama/ultraestrutura , Citoplasma/patologia , Citoplasma/ultraestrutura , Epitélio/ultraestrutura , Feminino , Vidro/química , Humanos , Dispositivos Ópticos , Sensibilidade e Especificidade , Água/química
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA