RESUMO
Gingival enlargement is a common clinical sign in the gingival diseases associated with orthodontic treatment. Its biological mechanisms are not completely understood; nevertheless, the biochemical changes associated with these inflammatory and overgrowth processes could alter the post-translational protein modifications occurring in various locations within the mouth. Here, changes in the profiles of the carbonylated and phosphorylated proteins in saliva were examined in donors with gingival enlargement (seven men and seven women) and healthy donors (six men and eight women). The sociodemographic characteristics of both groups did not present significant differences. Carbonylation was measured by a quantitative immunoassay (Dot Blot), whereas the profiles of the phosphorylated proteins were visualized by SDS-PAGE with quercetin staining. Some phosphopeptides were also identified using a typical LC-MS-MS approach. Our results showed that gingival enlargement induced a significant increase in oxidative damage in salivary proteins. While a significant reduction in phosphorylation was observed at the stain level in SDS-PAGE, there was a slight increase in the number of phosphorylated proteins identified by MS in samples with gingival enlargement.
RESUMO
Resumen Los concentrados de plaquetas son sustancias bioactivas autólogas, que tienen utilidad clínica en la regeneración tisular guiada. Este articulo muestra el caso de un paciente diagnosticado con periodontitis agresiva generalizada, quien fue medicado con doxiciclina e intervenido quirúrgicamente en dos tiempos, en la primera intervención se eliminó el tejido de granulación y agentes infecciosos locales a través de un raspaje y alisado radicular a campo abierto, en la segunda intervención, se realizó regeneración tisular guiada con aloinjerto y fibrina rica en plaquetas. En la reevaluación posquirúrgica, se valoró los cambios clínicos y radiográficos, observándose una disminución de la profundidad al sondaje, una mejoría en el nivel de inserción clínico y relleno óseo radiográfico cuando se compararon los datos basales y de seguimiento hasta por cuatro meses.
Abstract Platelet concentrates are autologous bioactive substances that have which has an important role clinical in the periodontal tissue regeneration. This article shows the case of a patient the patient had been diagnosed with aggressive periodontitis. The patient was treated by pharmacotherapy (docycline) and he underwent double surgeries. In the first surgical intervention was scaling and root planing with additional soft- tissue curettage. And the second surgical, tissue guided regeneration was performed using allograft and platelet rich plasma. Treatment generated satisfactory clinical outcomes in terms of probing depth reduction, clinical attachment level gain and radiographic bone fill. The data were recorded at base line, reevaluation, and 4 months post-surgery.
Resumo Os concentrados de plaquetas são substâncias bioativas autólogas que têm utilidade clínica na regeneração do tecido periodontal. Este artigo mostra o caso de um paciente diagnosticado com periodontite agressiva generalizada, medicado com doxiciclina e submetido a duas cirurgias. Na primeira intervenção, o tecido de granulação e os agentes infecciosos locais foram eliminados por raspagem em campo aberto e, na segunda intervenção, foi realizada regeneração tecidual guiada com aloenxerto e fibrina rica em plaquetas. Na reavaliação pós-cirúrgica, as alterações clínicas e radiográficas foram avaliadas, observando-se redução da profundidade da bolsa, melhor nível de inserção e preenchimento ósseo radiográfico quando comparados os dados basais e de acompanhamento por até quatro meses.
RESUMO
La hiperplasia epitelial focal es una de las patologías pertenecientes al grupo de infecciones causadas por el Virus del Papiloma Humano (VPH) relacionada con los tipos 13 y 32. Su transmisión es por contacto directo o indirecto con secreciones infectadas, siendo su grupo poblacional predilecto los niños, cuyo riesgo es mayor si presentan en su familia condiciones de salubridad insuficiente y malas prácticas de higiene. El tratamiento depende de la evolución de las lesiones que en la mayoría de los casos se resuelven espontáneamente, por lo que es suficiente con la observación. En caso de manifestaciones más severas se puede utilizar crioterapia, cirugía y acido acético (AU)
Focal epithelial dysplasia is one of the pathologies belonging to the group of infections caused by the Human Papilloma Virus (HPV), related to types 13 and 32. It is transmitted by direct or indirect contact with infected secretions, with its preferred population group being children, whose risk is greater if inadequate health conditions and bad hygiene practices are present in the family. Treatment is dependent on the evolution of lesions, which in the majority of cases remit spontaneously, in which case, observation is sufficient. In more severe cases, cryotherapy, surgery and acetic acid can be used (AU)
Assuntos
Humanos , Masculino , Criança , Hiperplasia Epitelial Focal/etiologia , Escovação Dentária/efeitos adversos , Papillomaviridae/patogenicidade , Infecções por Papillomavirus/transmissão , Higiene Bucal , Infecções/transmissão , Relações Profissional-FamíliaRESUMO
El granuloma telangiectásico se incluye dentro de las patologías reactivas inflamatorias orales asociadas a efectos secundarios de la ingesta de anovulatorios. Sus características clínicas no son patognomónicas, por lo que su diagnosis se debe realizar con apoyo en estudios histológicos. En cuanto a su incidencia, es mayor en mujeres que se encuentran en la segunda y tercera décadas de la vida. Aunque el factor etiológico aún es discutido, existe evidencia clara de que es una reacción que conjuga la presencia de factores irritantes locales, como la biopelícula, y condiciones sistémicas, como lo son las alteraciones hormonales (AU)
Pyogenic Granuloma is one of the reactive inflammatory oral diseases associated with contraceptive intake. The clinical features are not pathognomonic therefore its diagnosis must be made with histological support. Its incidence is higher in women who are in the second and third decade of life. Although its aetiology remains controversial there is clear evidence that it is a reaction to the presence of local irritants such as biofilms together with systemic conditions such as hormonal changes (AU)