Albendazole Release from Silica-Chitosan Nanospheres. In Vitro Study on Cervix Cancer Cell Lines.

In this work, a pH-responsive drug-carrier based on chitosan-silica nanospheres was developed as a carrier for Albendazole (ABZ), a poorly water-soluble anthelmintic drug. Spherical silica nanoparticles were obtained by Stöber method and further etched to obtain mesoporous particles with sizes ranging from 350 to 400 nm. The specific BET area of nanoparticles increased from 15 m2/g to 150 m2/g for etched silica, which also exhibited a uniform pore size distribution. X-ray powder diffraction showed the presence of amorphous phase of silica and a low-intensity peak attributed to ABZ for the drug-loaded nanoparticles. A uniform layer of chitosan was obtained ranging from 10 to 15 nm in thickness due to the small concentration of chitosan used (0.45 mg of chitosan/mg of SiO2). The in vitro evaluation of hybrid nanoparticles was performed using four cervical cancer cell lines CaSki, HeLa, SiHa and C33A, showing a significant reduction in cell proliferation (>85%) after 72 h. Therefore, we confirmed the encapsulation and bioavailability of the drug, which was released in a controlled way, and the presence of chitosan delayed the release, which could be of interest for the development of prolonged release drug delivery systems.

In vitro evaluation of apoptotic effect of bis(acetylacetonato-k2 O,O')(1,10-phenanthroline-k2 N,N')Zn(II) complex.

Phenanthroline derivatives have been reported as potential bioactive compounds because of their ability to interact with DNA. To evaluate the antiproliferative effect of bis(acetylacetonate-k2 O,O)(1,10-phenanthroline-k2 N,N)Zn(II) or Zn(acac)2 (phen) complex, the compound was obtained in a simple manner and further characterized to determine crystal structure, thermal behavior, morphology, and spectroscopic properties. The structure of the complex was confirmed by X-ray single structure as well as by 1H and 13C nuclear magnetic resonance (NMR) in dmso-d6 (dimethyl sulfoxide) solution and in the solid state by 13C CP/MAS. Although preparation of this compound has been described previously, there are no reports on its biological activity; here, we assessed its antiproliferative effect on fibroblasts, A253, FaDu, Cal-27, RH-30, RD, U-373, C6, A-549, MDA-MB-231, and MCF-7 cancer cell lines at different doses (50-100 and 150 µg/ml). The cell viability was determined by MTT assay and high activity was observed for the most of the cell lines, and TUNEL results showed the induction of apoptosis.

Intubación de secuencia rápida / Rapid-Sequence Intubation

En la práctica médica existen diversas situaciones que exigen inmediata permeabilización de la vía aérea en algunos pacientes, con el objetivo de garantizar una adecuada entrada y salida de gases a los pulmones y evitar la broncoaspiración. La intubación de secuencia rápida ha sido considerada como la administración de un agente hipnótico y un relajante neuromuscular de forma consecutiva (virtualmente simultánea) con el fin de facilitar la intubación orotraqueal en el paciente en estado crítico y minimizar el riesgo de aspiración. Este artículo pretende recopilar elementos que favorezcan una conducta médica acertada que obedezca a la situación presentada, pues no existe un patrón único de actuación ante la necesidad de intubación de secuencia rápida. Entre los elementos a tener en cuenta se destacan: contar con conocimientos anatómicos de las vías respiratorias superiores, disponer de un grupo de fármacos para elegir, tener entrenamiento adecuado y un plan alternativo ante la posibilidad de dificultades.

In medical practice there are several situations that require immediate intervention of the airway in some patients, in order to ensure proper entrance and exit of gases into and out of the lungs and prevent aspiration. Rapid-sequence intubation has been considered as the administration of a hypnotic agent and a neuromuscular relaxant consecutively (virtually simultaneously) to facilitate orotracheal intubation in critically ill patients and minimize the risk of aspiration. This paper aims to collect elements that promote a successful medical management according to the situation presented, since there is no single way of proceeding in case of rapid-sequence intubation. The elements to consider include: knowing the anatomy of the upper respiratory tract, having a group of drugs to choose from, receiving adequate training and having an alternative plan for the difficulties that may arise.

Anestesia analgesia multimodal en el superobeso / Multimodal analgesia anesthesia superobese

Introducción: el acto anestésico, en pacientes obesos, tiene sus requerimientos. La anestesia analgesia multimodal brinda ventajas a estos pacientes. Objetivos: describir la conducta perioperatoria de un paciente superobeso al que se le realizó cirugía bariátrica. Caso clínico: paciente de 40 años, con IMC 81,63 kg/m2, encamado durante 4 años por trauma en miembro inferior y exceso de peso. Apnea del sueño, diabetes mellitus tratado con Insulina lenta 60 U/día. Ventilación mecánica controlada por presión, volumen tidal de 8 ml/kg peso ideal. Se utilizaron varios medicamentos para conseguir analgesia. Extubado sin complicaciones. Conclusiones: la adecuada planificación y preparación del paciente a través de un equipo multidisciplinario y la aplicación de anestesia-analgesia multimodal favorecieron los resultados perioperatorios(AU)

Introduction: the anesthetic, in obese patients, has its requirements. Multimodal anesthesia-analgesia provides benefits to these patients.Methods: describe the behavior of a superobese perioperative patient who underwent bariatric surgery. Developing: 40 year old patient with BMI 81.63 kg / m2, bedding for 4 years in lower limb trauma and overweight. Sleep apnea, diabetes mellitus treated with insulin slow 60 U / day. Pressure controlled ventilation, tidal volume of 8 ml / kg ideal weight. Several drugs are used for analgesia. Extubated without complications. Conclusión: proper planning and preparation of the patient through a multidisciplinary team and the application of anesthesia and analgesia favored multimodal perioperative outcomes(AU)

Intubación de secuencia rápida / Rapid-sequence Intubation

En la práctica médica existen diversas situaciones que exigen inmediata permeabilización de la vía aérea en algunos pacientes, con el objetivo de garantizar una adecuada entrada y salida de gases a los pulmones y evitar la broncoaspiración. La intubación de secuencia rápida ha sido considerada como la administración de un agente hipnótico y un relajante neuromuscular de forma consecutiva (virtualmente simultánea) con el fin de facilitar la intubación orotraqueal en el paciente en estado crítico y minimizar el riesgo de aspiración. Este artículo pretende recopilar elementos que favorezcan una conducta médica acertada que obedezca a la situación presentada, pues no existe un patrón único de actuación ante la necesidad de intubación de secuencia rápida. Entre los elementos a tener en cuenta se destacan: contar con conocimientos anatómicos de las vías respiratorias superiores, disponer de un grupo de fármacos para elegir, tener entrenamiento adecuado y un plan alternativo ante la posibilidad de dificultades(AU)

n medical practice there are several situations that require immediate intervention of the airway in some patients, in order to ensure proper entrance and exit of gases into and out of the lungs and prevent aspiration. Rapid-sequence intubation has been considered as the administration of a hypnotic agent and a neuromuscular relaxant consecutively (virtually simultaneously) to facilitate orotracheal intubation in critically ill patients and minimize the risk of aspiration. This paper aims to collect elements that promote a successful medical management according to the situation presented, since there is no single way of proceeding in case of rapid-sequence intubation. The elements to consider include: knowing the anatomy of the upper respiratory tract, having a group of drugs to choose from, receiving adequate training and having an alternative plan for the difficulties that may arise(AU)