Mild hyperhomocysteinemia and the common C677T polymorphism of methylene tetrahydrofolate reductase gene are not associated with the metabolic syndrome in Type 2 diabetes.

A moderate increase of total homocysteine (tHcy) plasma levels seems to increase cardiovascular disease (CVD) risk in Type 2 diabetic subjects, but its relationship with diabetes and insulin-resistance is still controversial. We examined whether mild hyperhomocysteinemia and its major genetic determinant would cluster with the metabolic syndrome (MS) in Type 2 diabetes. One hundred Type 2 diabetic subjects with and without MS were enrolled in the study. Fasting tHcy, vitamin B12, and folate plasma levels, insulin-resistance [assessed by homeostasis model assessment, (HOMAIR)] and the methylene tetrahydrofolate reductase (MTHFR) C677T genotype were assessed in all the participants. Geometric mean tHcy concentration and the prevalence of mild hyperhomocysteinemia, as commonly defined by tHcy >/=15 micromol/l, were comparable in diabetic subjects with and without MS, even after adjustment for age, sex, vitamin B12, folate and creatinine levels. In both groups, the MTHFR C677T genotype distribution was not significantly different from the Hardy-Weinberg equilibrium, with a TT homozygous frequency of 21% in subjects with and 18% in those without the syndrome (p=ns). tHcy plasma levels and the degree of insulin-resistance did not differ across MTHFR genotypes in both groups, even after multivariable adjustment. Overall, tHcy significantly correlated with creatinine (r=0.25; p=0.009) and trygliceride concentrations (r=0.24; p=0.02), but not with HOMAIR. At multivariate analysis, only creatinine was significantly correlated with tHcy levels (beta=0.42; p=0.001). In conclusion, hyperhomocysteinemia and the common C677T variant of MTHFR gene are not associated with MS in Type 2 diabetic subjects.

[Survey on microclimatic condition of classroom]. / Indagine sulle condizioni microclimatiche delle aule scolastiche.

The results of a research, in winter and spring, on the microclimatic parameters and lighting in eighteen classrooms of primary schools in the town of Messina, are exposed. In a significant number of classrooms values of temperature and relative humidity are registered not in accordance with Italian standards. Thermal indices (PMV and PPD) showed, in both seasons, discomfort conditions in many rooms. A percentage of dissatisfied persons, significantly higher the 10% (56.8% in winter and 70.3% in spring), has been obtained. We used, also, a questionnaire modified by Fanger to evaluate the thermal subjective sensations of the students. The results point out that the thermal subjective sensations expressed by the students are often in disagreement with the Fanger thermal comfort indices obtained by instrument. The authors believe that the Fanger's indices do not reflect the effective environmental conditions.

[Serum levels of copper and zinc in patients with lung cancer]. / Livelli sierici di rame e zinco in pazienti con carcinoma del polmone.

Serum copper levels (SCL) and serum zinc levels (SZL) were measured in two groups of lung cancer patients divided according to disease extension. SCL was higher, SZL was lower and SCL/SZL ratio was more raised in patients extensively affected by the disease. It is confirmed that SCL, SZL and the SCL/SZL ratio play an important role in indicating the stage of lung cancer development.