RESUMO
OBJECTIVE: The aim of the present work was to evaluate the presence of human papillomavirus genotypes in malignant and normal mucosa of the colon and rectum in order to determine if a relationship exists between HPV infection and colon neoplasms. MATERIALS AND METHODS: Thirty normal colon tissues and 54 sporadic adenocarcinomas were screened for HPV positivity using nested-PCR. Detection of viral types 6, 11, 16, 18, 33, 34 and 51 was performed by the LIS-SSCP (Low Ionic Strength-Single Strand Conformational Polymorphism) procedure. RESULTS: Significant differences in high risk HPV infection were found between normal samples and adenocarcinomas (P < 0.001). Among the cases, an inverse association between HPV infection and Dukes staging was also found (P = 0.020). Finally, there was no significant association between HPV and some classical clinicopathological features, although a gradient of infection form rectum to cecum was evident. CONCLUSION: The present study demonstrates that HPV may infect the glandular mucosa of the colon and suggests a possible association between HPV and colorectal cancer.
Assuntos
Adenocarcinoma/virologia , Neoplasias do Colo/virologia , Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/diagnóstico , Neoplasias Retais/virologia , Adenocarcinoma/genética , Idoso , Idoso de 80 Anos ou mais , Distribuição de Qui-Quadrado , Neoplasias do Colo/genética , DNA Viral/análise , Feminino , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Papillomaviridae/genética , Infecções por Papillomavirus/genética , Reação em Cadeia da Polimerase , Polimorfismo Conformacional de Fita Simples , Neoplasias Retais/genéticaRESUMO
BACKGROUND: Helicobacter pylori infection is presumed to be the major causal agent of chronic active gastritis in humans. The persistent infection with this pathogen would be an important factor in the pathogenesis of peptic ulcer and also gastric cancer. METHODS: We investigated relationship between H. pylori characteristics in 42 patients with normal mucosa or gastritis with minor changes and 40 patients with mild and severe gastritis. Detection and typing of vacA and cagA genes were performed using a polymerase chain reaction method. RESULTS: The analysis of vacA prevalence and the type (S1 or S2) showed non-significant differences between the two groups studied (p > 0.05). However, cagA analysis showed highly significant differences between the groups classified as normal tissue-weak gastritis and mild-severe gastritis (p < 0.0001; OR = 8.4; CI = 3.1-22.8). CONCLUSIONS: cagA status is associated to the grade of gastritis, finding higher frequencies of H. pylori cagA+ in the moderate-severe gastritis group. These highly significant differences could make cagA status a genetic marker for disease progress.
Assuntos
Antígenos de Bactérias/genética , Proteínas de Bactérias/genética , Gastrite/microbiologia , Infecções por Helicobacter/microbiologia , Helicobacter pylori/genética , Reação em Cadeia da Polimerase , Adolescente , Adulto , Idoso , Antígenos de Bactérias/análise , Proteínas de Bactérias/análise , Proteínas de Bactérias/isolamento & purificação , Dispepsia/microbiologia , Dispepsia/patologia , Feminino , Gastrite/patologia , Marcadores Genéticos , Genótipo , Humanos , Mucosa Intestinal/microbiologia , Masculino , Pessoa de Meia-Idade , Estudos RetrospectivosRESUMO
BACKGROUND: Helicobacter pylori infection is presumed to be the major causal agent of chronic active gastritis in humans. The persistent infection with this pathogen would be an important factor in the pathogenesis of peptic ulcer and also gastric cancer. METHODS: We investigated relationship between H. pylori characteristics in 42 patients with normal mucosa or gastritis with minor changes and 40 patients with mild and severe gastritis. Detection and typing of vacA and cagA genes were performed using a polymerase chain reaction method. RESULTS: The analysis of vacA prevalence and the type (S1 or S2) showed non-significant differences between the two groups studied (p > 0.05). However, cagA analysis showed highly significant differences between the groups classified as normal tissue-weak gastritis and mild-severe gastritis (p < 0.0001; OR = 8.4; CI = 3.1-22.8). CONCLUSIONS: cagA status is associated to the grade of gastritis, finding higher frequencies of H. pylori cagA+ in the moderate-severe gastritis group. These highly significant differences could make cagA status a genetic marker for disease progress.
Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Antígenos de Bactérias/genética , Gastrite/microbiologia , Infecções por Helicobacter/microbiologia , Helicobacter pylori/genética , Reação em Cadeia da Polimerase , Antígenos de Bactérias/análise , Proteínas de Bactérias/genética , Proteínas de Bactérias/isolamento & purificação , Técnicas de Tipagem Bacteriana/métodos , DNA Bacteriano/análise , Dispepsia/microbiologia , Dispepsia/patologia , Mucosa Gástrica/microbiologia , Gastrite/patologia , Marcadores Genéticos , Genótipo , Helicobacter pylori/classificação , Helicobacter pylori/patogenicidade , Técnicas de Amplificação de Ácido Nucleico , Estudos Retrospectivos , VirulênciaRESUMO
BACKGROUND: Helicobacter pylori infection is presumed to be the major causal agent of chronic active gastritis in humans. The persistent infection with this pathogen would be an important factor in the pathogenesis of peptic ulcer and also gastric cancer. METHODS: We investigated relationship between H. pylori characteristics in 42 patients with normal mucosa or gastritis with minor changes and 40 patients with mild and severe gastritis. Detection and typing of vacA and cagA genes were performed using a polymerase chain reaction method. RESULTS: The analysis of vacA prevalence and the type (S1 or S2) showed non-significant differences between the two groups studied (p > 0.05). However, cagA analysis showed highly significant differences between the groups classified as normal tissue-weak gastritis and mild-severe gastritis (p < 0.0001; OR = 8.4; CI = 3.1-22.8). CONCLUSIONS: cagA status is associated to the grade of gastritis, finding higher frequencies of H. pylori cagA+ in the moderate-severe gastritis group. These highly significant differences could make cagA status a genetic marker for disease progress.
RESUMO
BACKGROUND: Helicobacter pylori infection is presumed to be the major causal agent of chronic active gastritis in humans. The persistent infection with this pathogen would be an important factor in the pathogenesis of peptic ulcer and also gastric cancer. METHODS: We investigated relationship between H. pylori characteristics in 42 patients with normal mucosa or gastritis with minor changes and 40 patients with mild and severe gastritis. Detection and typing of vacA and cagA genes were performed using a polymerase chain reaction method. RESULTS: The analysis of vacA prevalence and the type (S1 or S2) showed non-significant differences between the two groups studied (p > 0.05). However, cagA analysis showed highly significant differences between the groups classified as normal tissue-weak gastritis and mild-severe gastritis (p < 0.0001; OR = 8.4; CI = 3.1-22.8). CONCLUSIONS: cagA status is associated to the grade of gastritis, finding higher frequencies of H. pylori cagA+ in the moderate-severe gastritis group. These highly significant differences could make cagA status a genetic marker for disease progress.(AU)
Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Reação em Cadeia da Polimerase , Infecções por Helicobacter/microbiologia , Helicobacter pylori/genética , Gastrite/microbiologia , Antígenos de Bactérias/genética , Helicobacter pylori/classificação , Helicobacter pylori/patogenicidade , Técnicas de Tipagem Bacteriana/métodos , DNA Bacteriano/análise , Marcadores Genéticos , Virulência , Gastrite/patologia , Dispepsia/microbiologia , Dispepsia/patologia , Genótipo , Estudos Retrospectivos , Antígenos de Bactérias/análise , Mucosa Gástrica/microbiologia , Técnicas de Amplificação de Ácido Nucleico , Proteínas de Bactérias/genética , Proteínas de Bactérias/isolamento & purificaçãoAssuntos
Baço/microbiologia , Animais , Arenavirus do Novo Mundo/isolamento & purificação , Arenavirus do Novo Mundo/patogenicidade , Células Cultivadas , Cobaias , Interferons/biossíntese , Macrófagos/imunologia , Macrófagos/microbiologia , Masculino , Camundongos , Baço/imunologia , Baço/metabolismo , Vírus da Estomatite Vesicular Indiana/fisiologia , VirulênciaRESUMO
Approximately 80% of Calomys musculinus inoculated with an attenuated strain of Junin virus (JV) developed a lethal encephalitis. Antithymocyte serum, a potent suppressor of T-cell-mediated immunity, was studied for its effect on JV pathogenicity. Early administration of an anti-C. musculinus thymocyte serum (ACTS) to neonatal animals significantly diminished clinical disease and death and abrogated brain damage, which is usually associated with viral presence in the brain. Late ACTS administration did not modify the pattern of JV infection. These results suggest that immune mechanisms participate in the pathogenesis of JV infection for its main natural host.
Assuntos
Soro Antilinfocitário/uso terapêutico , Arenaviridae/imunologia , Arenavirus do Novo Mundo/imunologia , Arvicolinae/microbiologia , Febre Hemorrágica Americana/imunologia , Linfócitos T/imunologia , Animais , Arenavirus do Novo Mundo/crescimento & desenvolvimento , Arenavirus do Novo Mundo/patogenicidade , Linhagem Celular , Chlorocebus aethiops , Imunoterapia , Rim , Fatores de TempoRESUMO
In the present report, the techniques of isolation and morphological and functional characteristics of a subpopulation of human endometrial stromal cells are described. The data obtained point that these cells are macrophages, and that the uterine mucosa can be considered as a hormone-dependent territory of the monocyte-histiocytic system, since these cells can only be demonstrable during the proliferative or secretory phases of the uterine cycle, but not in atrophic, postmenopausal endometria.
Assuntos
Endométrio/citologia , Macrófagos/citologia , Adulto , Idoso , Células Cultivadas , Endométrio/imunologia , Feminino , Imunofluorescência , Humanos , Histerectomia , Macrófagos/imunologia , Macrófagos/ultraestrutura , Pessoa de Meia-Idade , Mucosa/imunologiaRESUMO
Infection of guinea pigs with an attenuated strain of Junin virus (JV) produced 16% mortality between days 17 and 27 postinfection (p.i.). The morphological study showed a marked pancreatitis between days 6 and 23 p.i. and meningoencephalitis between days 17 and 20 p.i. in a large proportion of the animals. These lesions were coincident with the presence of JV antigenic determinants in the pancreatic acinar cells, neurons and blood vessels of the brain. Infectious virus could be isolated from lymph nodes, spleen, bone marrow, lungs, adrenal glands, and brain. The lesions appeared to be reversible, as they were absent in animals studied after day 64 p.i. Meningoencephalitis, present in all animals dying spontaneously, appeared to be the most important cause of death. Our observations indicate that more accurate markers of virulence must be investigated in the search for attenuated strains of JV as potential vaccine candidates for Argentine hemorrhagic fever.
