Assuntos
Arginina/análogos & derivados , Infarto Cerebral/prevenção & controle , Ataque Isquêmico Transitório/prevenção & controle , Molsidomina/análogos & derivados , Molsidomina/farmacologia , Óxido Nítrico/metabolismo , Aminoácido Oxirredutases/antagonistas & inibidores , Análise de Variância , Animais , Arginina/farmacologia , Maleato de Dizocilpina/farmacologia , Ataque Isquêmico Transitório/metabolismo , Masculino , Camundongos , NG-Nitroarginina Metil Éster , Óxido Nítrico Sintase , Vasodilatadores/farmacologiaRESUMO
In the present study, we examine the involvement of the L-arginine-nitric oxide pathway in seizure activity termination. Convulsions were induced reproducibly by intracerebroventricular administration of N-methyl-D-aspartate to conscious mice. The duration of the seizure activity was increased by inhibition of the NO-pathway or by intracerebroventricular injection of methylene blue, an inhibitor of guanylate cyclase activity. This increased duration in seizure activity was reversed by co-administration of L-arginine or by intracerebroventricular injection of guanosine 3':5' cyclic monophosphate (cGMP). These results suggest that nitric oxide produced in response to NMDA receptor activation leads to an increase in cGMP which induces the seizure activity termination.