Towards total voluntary non-remunerated blood donation in Trinidad and Tobago

Objective: Investigate the historical origins of voluntary nonremunerated blood donation (VNRD) and describe a UWI-led initiative. Design and Methodology: Historical review was performed using internet searches, documents, books, journals, interviews. Data from blood donor cards and Microsoft Excel spreadsheets was collected prospectively and analysed retrospectively. Donors were classified by age, gender, donation status (first-time or repeat) and donation outcome (accepted or deferred). The prevalence of transfusion transmissible infections and deferrals in donors was compared to the national donor pool using Chi square analysis to compare proportions and a p value < 0.05 to assign statistical significance. Results: Human to human blood transfusion and voluntary non-remunerated blood donation were first practised in metropolitan countries and amplified in large scale community blood donation programmes during World War II. Blood donation systems based on individual, transactional donations emerged in most developing countries, including Trinidad and Tobago, causing low donation rates, chronic blood shortage, unequal access, high donor infections and high donor deferrals. A voluntary non-remunerated blood donation programme started by the UWI Blood Donor Foundation and the North Central Health Authority has collected 660 units of blood in its first three years, the majority from persons aged 16 -25 age (52%), females (52%) and repeat donors (51%). Deferrals were < 10% and total transfusion transmissible infections in donors 0.9% compared with 43.6% and 2.4 % respectively (p < 0.05 for both) for the involuntary national donor pool. Conclusion: This model could be extended to all blood donation centres and the community to achieve 100% VNRD.

C9orf72 is required for proper macrophage and microglial function in mice.

Expansions of a hexanucleotide repeat (GGGGCC) in the noncoding region of the C9orf72 gene are the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia. Decreased expression of C9orf72 is seen in expansion carriers, suggesting that loss of function may play a role in disease. We found that two independent mouse lines lacking the C9orf72 ortholog (3110043O21Rik) in all tissues developed normally and aged without motor neuron disease. Instead, C9orf72 null mice developed progressive splenomegaly and lymphadenopathy with accumulation of engorged macrophage-like cells. C9orf72 expression was highest in myeloid cells, and the loss of C9orf72 led to lysosomal accumulation and altered immune responses in macrophages and microglia, with age-related neuroinflammation similar to C9orf72 ALS but not sporadic ALS human patient tissue. Thus, C9orf72 is required for the normal function of myeloid cells, and altered microglial function may contribute to neurodegeneration in C9orf72 expansion carriers.

Effect of boron as an antidote on dry matter intake, nutrient utilization and fluorine balance in buffalo (Bubalus bubalis) exposed to high fluoride ration.

It is well known that excessive accumulation of fluorides can exert toxic effects on various tissues and organs so as to severely damage the health and production of animals. The aim of this study was to determine beneficial effect of boron on nutrient utilization in buffalo calves exposed to high fluoride (F) ration. For this purpose, we used three groups of four male Murrah buffalo calves (body weight 98-100 kg, aged 6-8 month) each. Control animal was given only basal diet and concentrate mixture. However, treatment I animals were fed basal diet, concentrate mixture, and F [as NaF, 60 ppm of dry matter (DM)]. The treatment II animals were fed basal diet, concentrate mixture, F (as NaF, 60 ppm of DM), and B (as sodium tetraborate, 140 ppm of DM). After 90 days of experimental feeding, a metabolism trial of 7 days duration was conducted to study the treatment effect on nutrient utilization of proximate nutrients, absorption, excretion, and retention of N, Ca, P, Fe, Zn, Cu, and F. Dietary F significantly (p < 0.05) depressed the dry matter intake and increased the apparent digestibility, absorption, and retention of F. However, boron supplementation significantly (p < 0.05) decreased the apparent digestibility, absorption, and retention of F and improved the dry matter intake, fecal excretion, and percent of absorbed F excreted via urine. Apparent digestibility of proximate nutrients (viz. DM, crude protein, crude fiber, ether extract, and nitrogen free extract) was unaffected on either F or F+B treatment. However, absorption and excretion of N, Ca, P, Fe, Zn, and Cu were affected significantly (p < 0.05) on F or F+B treatment. These findings suggest that fluoride-containing diet for short duration has effect on nutrient utilization, and boron at 140-ppm dose level, in general, antagonized the absorption and retention of F and also improved the feed intake in buffalo calves.

Ameliorative effects of boron on serum profile in buffalo (Bubalus bubalis) fed high fluoride ration.

An experiment was undertaken to evaluate the protective role of boron on the serum profile of buffalo calves fed a high fluoride ration. Twelve male Murrah buffalo (Bubalus bubalis) calves of 6-8 months age, divided into three groups of four calves in each, were fed basal diets and supplemented with sodium fluoride (NaF, 60 ppm) alone or in combination with borax (Na2B4O7.10H2O, 140 ppm) for 90 days. Boron (B) was added in the ration as borax to make @140 ppm boron (elemental B) on DM basis in treatment II. Dietary F caused a significant (p<0.05) depressing effect on serum Ca and Zn on day 90 which was improved with B supplementation. However, serum Fe and Cu did not show any significant change on F or F+B supplementation. The serum ALP and phosphorus level were increased significantly (p<0.05) on F feeding but declined significantly (p<0.05) when B was fed. The findings suggested beneficial effect of boron on serum minerals and ALP in buffalo calves fed high fluoride ration.

Regional pancreatectomy and transverse colectomy with mesenteric vascular reconstruction for inflammatory pseudotumour of the head of pancreas and mesenteric root.

BACKGROUND: Inflammatory myofibroblastic pseudotumour is a rare pancreatic lesion. CASE OUTLINE: A 32-year-old woman with such a tumour was treated by a radical operation comprising proximal pancreatic-duodenectomy (Whipple Procedure) and transverse colectomy with resection and reconstruction of the superior mesenteric artery and vein. She remains well 6 years later. DISCUSSION: The importance of aggressive surgical clearance rather than chemotherapy is highlighted in the management of patients with these unusual tumours.