RESUMO
Intramembrane proteolysis - cleavage of proteins within the plane of a membrane - is a widespread phenomenon that can contribute to the functional activation of substrates and is involved in several diseases. Although different families of intramembrane proteases have been discovered and characterized, we currently do not know how these enzymes discriminate between substrates and non-substrates, how site-specific cleavage is achieved, or which factors determine the rate of proteolysis. Focusing on γ-secretase and rhomboid proteases, we argue that answers to these questions may emerge from connecting experimental readouts, such as reaction kinetics and the determination of cleavage sites, to the structures and the conformational dynamics of substrates and enzymes.
Assuntos
Proteínas de Membrana/química , Peptídeo Hidrolases/metabolismo , Proteólise , Secretases da Proteína Precursora do Amiloide/química , Membrana Celular/metabolismo , Humanos , Cinética , Proteínas de Membrana/metabolismo , Peptídeo Hidrolases/químicaRESUMO
LV-peptides were designed as membrane-spanning low-complexity model structures that mimic fusion protein transmembrane domains. These peptides harbor a hydrophobic core sequence that consists of helix-promoting and helix-destabilizing residues at different ratios. Previously, the fusogenicity of these peptides has been shown to increase with the conformational flexibility of their hydrophobic cores as determined in isotropic solution. Here, we examined the secondary structure, orientation, and distribution of LV-peptides in membranes. Our results reveal that the peptides are homogeneously distributed within the membranes of giant unilamellar liposomes and capable of fusing them. Increasing the valine content of the core up to the level of the beta-branched residue content of SNARE TMDs (approximately 50%) enhances fusogenicity while maintaining a largely alpha-helical structure in liposomal membranes. A further increase in valine content or introduction of a glycine/proline pair favors beta-sheet formation. In planar bilayers, the alpha-helices adopt oblique angles relative to the bilayer normal and the ratio of alpha-helix to beta-sheet responds more sensitively to valine content. We propose that the fusogenic conformation of LV-peptides is likely to correspond to a membrane-spanning alpha-helix. Beta-sheet formation in membranes may be considered a side-reaction whose extent reflects conformational flexibility of the core.
Assuntos
Bicamadas Lipídicas/química , Peptídeos/química , Sequência de Aminoácidos , Centrifugação com Gradiente de Concentração , Dicroísmo Circular , Espectroscopia de Ressonância de Spin Eletrônica , Lipídeos/química , Lipossomos/química , Microscopia Confocal , Dados de Sequência Molecular , Conformação Proteica , Estrutura Secundária de Proteína , Solventes , Espectroscopia de Infravermelho com Transformada de Fourier , Sacarose/químicaRESUMO
Biological membrane fusion is driven by different types of molecular fusion machines. Most of these proteins are membrane-anchored by single transmembrane domains. SNARE proteins are essential for intracellular membrane fusion along the secretory and endocytic pathway, while various viral fusogens mediate infection of eukaryotic cells by enveloped viruses. Although both types of fusion proteins are evolutionarily quite distant from each other, they do share a number of structural and functional features. Their transmembrane domains are now known to be critical for the fusion reaction. We discuss at which stages they might contribute to bilayer mixing.
Assuntos
Fusão de Membrana , Proteínas de Membrana/metabolismo , Animais , Membrana Celular/química , Membrana Celular/metabolismo , Humanos , Bicamadas Lipídicas/química , Bicamadas Lipídicas/metabolismo , Proteínas de Membrana/química , Estrutura Terciária de Proteína , Proteínas SNARE/química , Proteínas SNARE/metabolismoRESUMO
Intracellular membrane fusion in eukaryotic cells is mediated by SNARE (soluble N-ethylmaleimide sensitive factor (NSF) attachment protein receptor) proteins and is known to involve assembly of cognate subunits to heterooligomeric complexes. For synaptic SNAREs, it has previously been shown that the transmembrane segments drive homotypic and support heterotypic interactions. Here, we demonstrate that a significant fraction of the yeast vacuolar SNARE Vam3p is a homodimer in detergent extracts of vacuolar membranes. This homodimer exists in parallel to the heterooligomeric SNARE complex. A Vam3p homodimer also formed from the isolated recombinant protein. Interestingly, homodimerization depended on the transmembrane segment. In contrast, formation of the quaternary SNARE complex from recombinant Vam3p, Nyv1p, Vti1p, and Vam7p subunits did not depend on the transmembrane segment of Vam3p nor on the transmembrane segments of its partner proteins. We conclude that Vam3p homodimerization, but not quaternary SNARE complex formation, is promoted by TMS-TMS interaction. As the transmembrane segments of Vam3p and other SNARE homologues were previously shown to be critical for membrane fusion downstream of membrane apposition, our results may shed light on the functional significance of SNARE TMS-TMS interactions.
Assuntos
Proteínas Fúngicas/metabolismo , Membranas Intracelulares/metabolismo , Proteínas de Membrana/metabolismo , Proteínas Qa-SNARE/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Proteínas de Ligação a Fator Solúvel Sensível a N-Etilmaleimida/metabolismo , Dimerização , Proteínas Fúngicas/química , Proteínas Fúngicas/genética , Técnicas In Vitro , Proteínas de Membrana/genética , Proteínas de Membrana/isolamento & purificação , Proteínas Qa-SNARE/química , Proteínas Qa-SNARE/genética , Proteínas Recombinantes/metabolismo , Proteínas de Saccharomyces cerevisiae/química , Proteínas de Saccharomyces cerevisiae/genética , Proteínas de Ligação a Fator Solúvel Sensível a N-Etilmaleimida/genéticaRESUMO
A membrane-spanning heptad repeat motif mediates interaction between transmembrane segments. This motif was randomized with three different sets of mostly hydrophobic residues in the context of POSSYCCAT, a modified ToxR transcription activator system. The resulting combinatorial libraries were subjected to different levels of selective pressure to obtain groups of transmembrane segments that are distinguished by their ability to self-interact in bacterial membranes. Upon relating self-interaction to amino acid composition, the following conclusions were made. First, randomization with only Leu, Ile, Val, Met, and Phe resulted in unexpected robust self-interaction with little sequence specificity. Second, with more complex amino acid mixtures that represent natural transmembrane segments more closely, self-interaction critically depended on amino acid composition of the interface. Whereas the contents of Ile and Leu residues increased with the ability to self-interact, the contents of Pro and Arg residues decreased. Third, heptad repeat motifs composed of Leu, Ile, Val, Met, and Phe were approximately 40-fold over-represented in transmembrane segments of single-span membrane proteins as compared with motifs composed of the more complex amino acid mixtures. This suggests that heptad motifs composed of the smaller subset of amino acids were enriched in the course of natural single-span membrane protein evolution.
Assuntos
Proteínas de Bactérias , Proteínas de Ligação a DNA/metabolismo , Zíper de Leucina , Proteínas de Membrana/metabolismo , Fatores de Transcrição/metabolismo , Motivos de Aminoácidos , Técnicas de Química Combinatória , Primers do DNA , Proteínas de Ligação a DNA/química , Proteínas de Ligação a DNA/genética , Proteínas de Membrana/química , Proteínas de Membrana/genética , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Ligação Proteica , Fatores de Transcrição/química , Fatores de Transcrição/genéticaRESUMO
SNARE proteins are essential for different types of intracellular membrane fusion. Whereas interaction between their cytoplasmic domains is held responsible for establishing membrane proximity, the role of the transmembrane segments in the fusion process is currently not clear. Here, we used an in vitro approach based on lipid mixing and electron microscopy to examine a potential fusogenic activity of the transmembrane segments. We show that the presence of synthetic peptides representing the transmembrane segments of the presynaptic soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) synaptobrevin II (also referred to as VAMP II) or syntaxin 1A, but not of an unrelated control peptide, in liposomal membranes drives their fusion. Liposome aggregation by millimolar Ca(2+) concentrations strongly potentiated the effect of the peptides; this indicates that juxtaposition of the bilayers favours their fusion in the absence of the cytoplasmic SNARE domains. Peptide-driven fusion is reminiscent of natural membrane fusion, since it was suppressed by lysolipid and involved both bilayer leaflets. This suggests transient presence of a hemifusion intermediate followed by complete membrane merger. Structural studies of the peptides in lipid bilayers performed by Fourier transform infrared spectroscopy indicated mixtures of alpha-helical and beta-sheet conformations. In isotropic solution, circular dichroism spectroscopy showed the peptides to exist in a concentration-dependent equilibrium of alpha-helical and beta-sheet structures. Interestingly, the fusogenic activity decreased with increasing stability of the alpha-helical solution structure for a panel of variant peptides. Thus, structural plasticity of transmembrane segments may be important for SNARE protein function at a late step in membrane fusion.
Assuntos
Fusão de Membrana , Proteínas de Membrana/química , Proteínas de Membrana/metabolismo , Mimetismo Molecular , Peptídeos/química , Peptídeos/metabolismo , Proteínas de Transporte Vesicular , Sequência de Aminoácidos , Cálcio/metabolismo , Dicroísmo Circular , Bicamadas Lipídicas/química , Bicamadas Lipídicas/metabolismo , Lipossomos/química , Lipossomos/metabolismo , Microscopia Eletrônica , Dados de Sequência Molecular , Maleabilidade , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Proteínas Qa-SNARE , Proteínas R-SNARE , Proteínas SNARE , Soluções , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
The efficiency of cell-cell fusion mediated by heterologously expressed vesicular stomatitis virus G-protein has previously been shown to be affected by mutating its transmembrane segment. Here, we show that a synthetic peptide modeled after this transmembrane segment drives liposome-liposome fusion. Addition of millimolar Ca(2+) concentrations strongly potentiated the effect of the peptides suggesting that Ca(2+)-mediated liposome aggregation supports the activity of the peptide. Peptide-driven fusion was suppressed by lysolipid, an established inhibitor of natural membrane fusion, and involved inner and outer leaflets of the liposomal bilayer. Thus, transmembrane segment peptide-driven liposome fusion exhibits important hallmarks characteristic of natural membrane fusion. Importantly, the mutations previously shown to attenuate the function of full-length G-protein in cell-cell fusion also attenuated the fusogenicity of the peptide, albeit in a less pronounced fashion. Therefore, the function of the peptide mimic is dependent on its primary structure, similar to full-length G-protein. Together, our data suggest that the G-protein transmembrane segment is an autonomous functional domain. We propose that it acts at a late step in membrane fusion elicited by vesicular stomatitis virus.
Assuntos
Glicoproteínas/metabolismo , Fusão de Membrana , Glicoproteínas de Membrana , Mimetismo Molecular , Peptídeos/metabolismo , Vírus da Estomatite Vesicular Indiana/metabolismo , Proteínas do Envelope Viral/metabolismo , Sequência de Aminoácidos , Cálcio/metabolismo , Glicoproteínas/química , Glicoproteínas/genética , Lipossomos , Dados de Sequência Molecular , Peptídeos/química , Mutação Puntual , Espectrometria de Fluorescência , Proteínas do Envelope Viral/química , Proteínas do Envelope Viral/genéticaRESUMO
High-level heterologous expression of integral membrane proteins at full-length is a useful tool for their structural and functional characterization. Here, systems that have previously been used for efficient bacterial expression of eukaryotic membrane proteins are reviewed and novel vectors consisting of a modular fusion moiety based on nuclease A from Staphylococcus aureus are presented.
Assuntos
Bactérias/genética , Proteínas de Membrana/biossíntese , Proteínas Recombinantes de Fusão/biossíntese , Proteínas de Transporte Vesicular , Sequência de Aminoácidos , Animais , Bactérias/metabolismo , Sequência de Bases , Expressão Gênica , Técnicas Genéticas , Vetores Genéticos , Immunoblotting , Proteínas de Membrana/genética , Dados de Sequência Molecular , Proteínas R-SNARE , Proteínas Recombinantes de Fusão/genética , Proteínas SNARERESUMO
Hematopoietic cytokine receptors, such as the erythropoietin receptor (EpoR), are single membrane-spanning proteins. Signal transduction through EpoR is crucial for the formation of mature erythrocytes. Structural evidence shows that in the unliganded form EpoR exists as a preformed homodimer in an open scissor-like conformation precluding the activation of signaling. In contrast to the extracellular domain of the growth hormone receptor (GHR), the structure of the agonist-bound EpoR extracellular region shows only minimal contacts between the membrane-proximal regions. This evidence suggests that the domains facilitating receptor dimerization may differ between cytokine receptors. We show that the EpoR transmembrane domain (TM) has a strong potential to self interact in a bacterial reporter system. Abolishing self assembly of the EpoR TM by a double point mutation (Leu 240-Leu 241 mutated to Gly-Pro) impairs signal transduction by EpoR in hematopoietic cells and the formation of erythroid colonies upon reconstitution in erythroid progenitor cells from EpoR(-/-) mice. Interestingly, inhibiting TM self assembly in the constitutively active mutant EpoR R129C abrogates formation of disulfide-linked receptor homodimers and consequently results in the loss of ligand-independent signal transduction. Thus, efficient signal transduction through EpoR and possibly other preformed receptor oligomers may be determined by the dynamics of TM self assembly.
Assuntos
Receptores da Eritropoetina/metabolismo , Transdução de Sinais , Sequência de Aminoácidos , Animais , Western Blotting , Linhagem Celular , Membrana Celular/metabolismo , Camundongos , Dados de Sequência Molecular , Mutação , Plasmídeos , Testes de Precipitina , Receptores da Eritropoetina/química , Receptores da Eritropoetina/genética , Homologia de Sequência de AminoácidosRESUMO
Assembly of the SNARE proteins synaptobrevin/VAMP, syntaxin, and SNAP-25 to binary and ternary complexes is important for docking and/or fusion of presynaptic vesicles to the neuronal plasma membrane prior to regulated neurotransmitter release. Despite the well characterized structure of their cytoplasmic assembly domains, little is known about the role of the transmembrane segments in SNARE protein assembly and function. Here, we identified conserved amino acid motifs within the transmembrane segments that are required for homodimerization of synaptobrevin II and syntaxin 1A. Minimal motifs of 6-8 residues grafted onto an otherwise monomeric oligoalanine host sequence were sufficient for self-interaction of both transmembrane segments in detergent solution or membranes. These motifs constitute contiguous areas of interfacial residues assuming alpha-helical secondary structures. Since the motifs are conserved, they also contributed to heterodimerization of synaptobrevin II and syntaxin 1A and therefore appear to constitute interaction domains independent of the cytoplasmic coiled coil regions. Interactions between the transmembrane segments may stabilize the SNARE complex, cause its multimerization to previously observed multimeric superstructures, and/or be required for the fusogenic activity of SNARE proteins.
Assuntos
Proteínas de Membrana/química , Proteínas de Membrana/metabolismo , Proteínas do Tecido Nervoso/química , Proteínas do Tecido Nervoso/metabolismo , Proteínas de Transporte Vesicular , Sequência de Aminoácidos , Animais , Sítios de Ligação , Sequência Conservada , Reagentes de Ligações Cruzadas , Dimerização , Substâncias Macromoleculares , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Estrutura Secundária de Proteína , Proteínas Qa-SNARE , Proteínas R-SNARE , Ratos , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Proteínas SNARE , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Proteína 25 Associada a Sinaptossoma , Sintaxina 1RESUMO
Lateral clustering of E-cadherin molecules is required for the adhesive properties of this cell-cell adhesion molecule. Both the extracellular domain and the cytoplasmic region of E-cadherin were previously reported to contribute to lateral clustering, but little is known about a role of the transmembrane domain in this respect. Following our previous findings indicating self-assembly of artificial transmembrane segments based on leucine residues, we asked whether the leucine-rich transmembrane segment of E-cadherin participates in lateral clustering. Here, we demonstrate that its transmembrane domain self-assembles as analyzed using the ToxR reporter system. Certain point mutations within the transmembrane domain markedly reduced self-assembly. To study whether the same point mutations also affect E-cadherin-mediated adhesion in vivo, wild-type and mutant E-cadherin cDNAs were transfected into Ltk(-) cells. Indeed, cell aggregation assays revealed significantly reduced adhesiveness when mutations had been introduced which disrupted transmembrane segment interaction. In control experiments, cell-surface expression, interaction with catenins and the cytoskeleton as well as trypsin-resistance of the protein were unaffected. These data suggest that interactions between the transmembrane segments are important for the lateral association of E-cadherin molecules required for cell-cell adhesion.
Assuntos
Proteínas de Bactérias , Caderinas/genética , Caderinas/metabolismo , Adesão Celular/fisiologia , Sequência de Aminoácidos , Animais , Caderinas/química , Agregação Celular , Proteínas de Ligação a DNA/genética , Genes Reporter , Células L , Camundongos , Modelos Moleculares , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Proteínas Recombinantes de Fusão/biossíntese , Fatores de Transcrição/genética , Ativação Transcricional , TransfecçãoRESUMO
Specific interactions between alpha-helical transmembrane segments are important for folding and/or oligomerization of membrane proteins. Previously, we have shown that most transmembrane helix-helix interfaces of a set of crystallized membrane proteins are structurally equivalent to soluble leucine zipper interaction domains. To establish a simplified model of these membrane-spanning leucine zippers, we studied the homophilic interactions of artificial transmembrane segments using different experimental approaches. Importantly, an oligoleucine, but not an oligoalanine, se- quence efficiently self-assembled in membranes as well as in detergent solution. Self-assembly was maintained when a leucine zipper type of heptad motif consisting of leucine residues was grafted onto an alanine host sequence. Analysis of point mutants or of a random sequence confirmed that the heptad motif of leucines mediates self-recognition of our artificial transmembrane segments. Further, a data base search identified degenerate versions of this leucine motif within transmembrane segments of a variety of functionally different proteins. For several of these natural transmembrane segments, self-interaction was experimentally verified. These results support various lines of previously reported evidence where these transmembrane segments were implicated in the oligomeric assembly of the corresponding proteins.
Assuntos
Proteínas de Bactérias , Zíper de Leucina/genética , Leucina/metabolismo , Proteínas de Membrana/metabolismo , Sequência de Aminoácidos , Animais , Galinhas , Cromatografia em Gel , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Bases de Dados Factuais , Humanos , Proteínas de Membrana/genética , Camundongos , Dados de Sequência Molecular , Mutação Puntual , Relação Estrutura-Atividade , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Ativação Transcricional , XenopusRESUMO
Membrane-embedded protein domains frequently exist as alpha-helical bundles, as exemplified by photosynthetic reaction centers, bacteriorhodopsin, and cytochrome C oxidase. The sidechain packing between their transmembrane helices was investigated by a nearest-neighbor analysis which identified sets of interfacial residues for each analyzed helix-helix interface. For the left-handed helix-helix pairs, the interfacial residues almost exclusively occupy positions a, d, e, or g within a heptad motif (abcdefg) which is repeated two to three times for each interacting helical surface. The connectivity between the interfacial residues of adjacent helices conforms to the knobs-into-holes type of sidechain packing known from soluble coiled coils. These results demonstrate on a quantitative basis that the geometry of sidechain packing is similar for left-handed helix-helix pairs embedded in membranes and coiled coils of soluble proteins. The transmembrane helix-helix interfaces studied are somewhat less compact and regular as compared to soluble coiled coils and tolerate all hydrophobic amino acid types to similar degrees. The results are discussed with respect to previous experimental findings which demonstrate that specific interactions between transmembrane helices are important for membrane protein folding and/or oligomerization.
Assuntos
Proteínas de Membrana/química , Estrutura Secundária de Proteína , Algoritmos , Sequência de Aminoácidos , Bacteriorodopsinas/química , Complexo IV da Cadeia de Transporte de Elétrons/química , Dados de Sequência Molecular , Complexo de Proteínas do Centro de Reação Fotossintética/química , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , SolubilidadeRESUMO
The glycophorin A transmembrane segment homo-dimerizes to a right-handed pair of alpha-helices. Here, we identified the amino acid motif mediating this interaction within a natural membrane environment. Critical residues were grafted onto two different hydrophobic host sequences in a stepwise manner and self-assembly of the hybrid sequences was determined with the ToxR transcription activator system. Our results show that the motif LIxxGxxxGxxxT elicits a level of self-association equivalent to that of the original glycophorin A transmembrane segment. This motif is very similar to the one previously established in detergent solution. Interestingly, the central GxxxG motif by itself already induced strong self-assembly of host sequences and the three-residue spacing between both glycines proved to be optimal for the interaction. The GxxxG element thus appears to be the most crucial part of the interaction motif.
Assuntos
Proteínas de Bactérias , Glicoforinas/química , Conformação Proteica , Divisão Celular/genética , Linhagem Celular , Proteínas de Ligação a DNA/metabolismo , Dimerização , Glicina/química , Maltose/metabolismo , Proteínas de Membrana/química , Regiões Promotoras Genéticas/genética , Engenharia de Proteínas/métodos , Estrutura Secundária de Proteína , Proteínas Recombinantes de Fusão/química , Fatores de Transcrição/metabolismo , Ativação Transcricional/genéticaRESUMO
Synaptobrevin is an integral membrane protein of presynaptic vesicles and is essential for neurotransmitter release. Previously, a dimeric quaternary structure has been proposed by cross-linking experiments performed on brain fractions. Here, we demonstrate that heterologously expressed and solubilized synaptobrevin II forms a homodimer. The dimers were detected upon cross-linking with a homobifunctional lysine-reactive reagent or by oxidation of the single cysteine residue located within the transmembrane segment. Dimerization was also observed without prior cross-linking upon SDS/PAGE under mild conditions. Interestingly, dimerization required the presence of the transmembrane segment which therefore is inferred to be the principal site of subunit-subunit interaction. The residues comprizing this segment were individually mutated. Dimerization of some point mutants was significantly impaired, which proved the sequence specificity of interaction and identified residues contributing to the subunit-subunit interface. The distribution of these residues (Leu99, Ile102, Cys103, Leu107, Ile110, and Ile111) suggests that the transmembrane segment has an alpha-helical structure and that the helices pair in a right-handed fashion. The importance of the transmembrane segment for subunit-subunit interaction relates synaptobrevin to fusogenic membrane proteins of enveloped viruses where transmembrane segments have been implicated in both oligomerization and membrane fusion.
Assuntos
Proteínas de Membrana/biossíntese , Proteínas de Membrana/química , Estrutura Secundária de Proteína , Vesículas Sinápticas/metabolismo , Sequência de Aminoácidos , Animais , Clonagem Molecular , Reagentes de Ligações Cruzadas , Cisteína , Dimerização , Escherichia coli , Lisina , Substâncias Macromoleculares , Proteínas de Membrana/isolamento & purificação , Modelos Estruturais , Dados de Sequência Molecular , Oxirredução , Proteínas R-SNARE , Ratos , Proteínas Recombinantes de Fusão/biossíntese , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/isolamento & purificaçãoRESUMO
Specific interactions between membrane spanning polypeptide segments are important for folding and oligomerisation of integral membrane proteins. Previously the dimerisation of glycophorin A has been shown to depend on interactions between its transmembrane segment by studying chimeric proteins in detergent solution. Here, we examined dimerisation of the glycophorin A transmembrane segment in a natural membrane employing the ToxR transcription activator from Vibrio cholerae. The ToxR protein is integral to the bacterial inner membrane and its activity requires a dimeric state. Therefore, the ToxR protein is suited to monitor quantitative homophilic interactions. We replaced the ToxR transmembrane segment with parts of the glycophorin A transmembrane segment containing the amino acid motif LIxxGVxxGVxxT previously shown to be sufficient for dimerisation in detergent solution. Expression of these chimeric proteins in an indicator strain resulted in strong transcription activation. This is indicative of efficient dimerisation mediated by the glycophorin transmembrane segment inserted into the inner membrane. Analysis of individual point mutants revealed that at least four residues out of this motif are critical for dimer formation in membranes. However, dimerisation of the glycophorin A transmembrane segment appears to be less sensitive to mutations when localised within a natural lipid bilayer compared to measurements in detergent solution. This may be related to a slightly altered structure of the dimer and/or to a higher local concentration and preorientation of the interacting molecules in a membrane. This makes the ToxR system well suited for probing low-affinity interactions between the transmembrane segments of other proteins.
Assuntos
Proteínas de Bactérias , Membrana Celular/metabolismo , Proteínas de Ligação a DNA/metabolismo , Glicoforinas/química , Glicoforinas/metabolismo , Fatores de Transcrição/metabolismo , Sequência de Aminoácidos , Proteínas de Ligação a DNA/genética , Escherichia coli/genética , Escherichia coli/metabolismo , Glicoforinas/genética , Dados de Sequência Molecular , Mutação , Conformação Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Fatores de Transcrição/genética , Ativação TranscricionalRESUMO
The tubulin-binding protein gephyrin copurifies with the inhibitory glycine receptor (GlyR) and is essential for its postsynaptic localization. Here we have analyzed the interaction between the GlyR and recombinant gephyrin and identified a gephyrin binding site in the cytoplasmic loop between the third and fourth transmembrane segments of the beta subunit. GlyR alpha subunits and GABAA receptor proteins failed to bind recombinant gephyrin. However, insertion of an 18 residue segment of the GlyR beta subunit into the GABAA receptor beta 1 subunit conferred gephyrin binding both in an overlay assay and in transfected mammalian cells. These results indicate that beta subunit expression is essential for the formation of a postsynaptic GlyR matrix.
Assuntos
Proteínas de Transporte/metabolismo , Proteínas de Membrana/metabolismo , Receptores de Glicina/química , Sequência de Aminoácidos , Sequência de Bases , Sítios de Ligação , Linhagem Celular , Eletroforese em Gel de Poliacrilamida , Embrião de Mamíferos , Expressão Gênica , Humanos , Rim , Substâncias Macromoleculares , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Receptores de GABA/genética , Receptores de GABA/metabolismo , Receptores de Glicina/genética , Receptores de Glicina/metabolismo , Proteínas Recombinantes/metabolismo , Homologia de Sequência , TransfecçãoRESUMO
The glycine receptor is a ligand-gated anion channel protein of postsynaptic membranes. We expressed a homo-oligomeric receptor composed of human alpha 1 subunits in Spodoptera frugiperda cells by infection with a recombinant Autographa californica nuclear polyhedrosis virus. A substantial fraction of the recombinant receptor was incorporated as a functional channel protein into the cell's plasma membrane at expression levels 4- to 30-fold higher than in other eukaryotic heterologous expression systems or native rat spinal cord membranes, respectively. Upon detergent solubilization, the alpha 1 receptor was found to exist in a predominantly monodisperse state and could be affinity-purified to near homogeneity. This preparation is a potential starting point for future crystallisation studies.
Assuntos
Receptores de Glicina/genética , Animais , Sequência de Bases , Biopolímeros , Western Blotting , Linhagem Celular , Cromatografia de Afinidade , Clonagem Molecular , Eletroforese em Gel de Poliacrilamida , Dados de Sequência Molecular , Nucleopoliedrovírus/genética , Oligodesoxirribonucleotídeos , Receptores de Glicina/isolamento & purificação , Receptores de Glicina/metabolismo , Solubilidade , SpodopteraRESUMO
beta-Alanine and taurine are agonists of the glycine receptor (GlyR) which, at low concentrations, antagonize the action of the principal agonist glycine. We analysed the potency of these ligands on alpha 1 subunits mutated at residue R271. GlyRs formed from alpha 1R271K subunits showed a reduction of beta-alanine and taurine affinities and maximal inducible currents; the mutants alpha 1R271Q and alpha 1R271L associated with human hyperekplexia gave no responses to these ligands. Inhibition of glycine-evoked currents by beta-alanine and taurine, however, was similar for all mutant GlyRs. These data are consistent with the existence of two subdomains within the ligand binding region of the GlyR, an agonistic one, which depends on arginine 271, and an antagonistic subsite, which is not connected to this residue.
Assuntos
Doenças do Sistema Nervoso/genética , Receptores de Aminoácido/metabolismo , Receptores de Glicina/metabolismo , Animais , Glicina/antagonistas & inibidores , Glicina/farmacologia , Humanos , Ligantes , Membranas/metabolismo , Mutação , Doenças do Sistema Nervoso/metabolismo , Oócitos/metabolismo , RNA/metabolismo , Receptores de Aminoácido/agonistas , Receptores de Aminoácido/genética , Receptores de Glicina/agonistas , Receptores de Glicina/genética , Taurina/farmacologia , Xenopus laevis , beta-Alanina/farmacologiaRESUMO
Hereditary hyperekplexia is a dominant neurological disorder associated with point mutations at the channel-forming segment M2 of the glycine receptor alpha 1 subunit. Voltage-clamp recordings from the heterologously expressed mutants (alpha 1R271L or alpha 1R271Q) revealed 146- to 183-fold decreased potencies of glycine to activate the chloride channel, and significantly reduced maximal whole-cell currents as compared with wild-type receptors. In contrast, the ability of the competitive antagonist strychnine to block glycine-induced currents was similar in all cases. Radioligand binding assays showed a 90- to 1365-fold reduction in the ability of glycine to displace [3H]strychnine from its binding site on the mutant receptors. Paralleling the reductions in whole-cell current, the elementary main-state conductances of the mutants (alpha 1R271L, 64 pS; alpha 1R271Q, 14 pS) were lower than that of the wild-type receptor (86 pS). The decreased agonist affinities and chloride conductances of the mutants are likely to cause neural hyperexcitability of affected patients by impairing glycinergic inhibition. In addition, our data reveal that structural modifications of the ion-channel region can affect agonist binding to the glycine receptor.