Expressions of HIF-1α and HSP70 in placenta tissue of patients with pregnancy-induced hypertension syndrome and their significances / 吉林大学学报(医学版)

Objective:To investigate the expressions of hypoxia-inducible factor-1α(HIF-1α)and heat shock protein 70(HSP70)in the placenta tissue of the patients with pregnancy-induced hypertension syndrome(PIH), and to elucidate the clinical significances of their expressions in the placenta tissue of the patients with PIH. Methods:Twenty-two cases of placenta tissue of the PIH patients(PIH group)and eighteen cases of placenta tissue of the normal pregnant women(control group)were selected.The expressions of HIF-1αand HSP70 were detected by immunohistochemical method.The differences of potive expressions rates of HIF-1α and HSP70 in placenta tissue of the PIH patients and the normal pregnant women and their relationships in placenta tissue of the PIH patients were analyzed.Results:The positive expression rate of HIF-1α in placenta tissue of the patients in PIH group(81.8%)was higher than that of the normal pregnant women in control group(38.9%)(χ2=7.785,P=0.005),and the positive expression rate of HSP70 in placenta tissue of the patients in PIH group(90.9%)was higher than that of the normal pregnant women in control group(55.6%)(χ2=6.599,P=0.010).Eighteen cases of placenta tissue with HIF-1α positive expression in the PIH patients had HSP70 positive expression(100.0%);among four cases of placenta tissue with HIF-1α negative expression,two cases had HSP70 negative expression (50.0%);their expressions in placenta tissue of the PIH patients had positive correlation(r=0.671,P=0.001). Conclusion:The expressions of HIF-1αand HSP70 in placenta tissue of the PIH patients are higher,and they have positive correlation.

Identification, structure and function of a novel tetraspaninhomologue from Spirometra erinaceieuropaei.

OBJECTIVE: To identify a full length c DNA sequence of a novel tetraspanin (TSP) homologue from Spirometra erinaceieuropaei and to predict the structure and function of its encoding protein using bioinformatics methods. METHODS: Using the NCBI, EMBI, Expasy and other online sites, the open reading frame (ORF), conserved domain, physical and chemical parameters, signal peptide, transmembrane domain, epitope, topological structures of the protein sequences were predicted. And Vector NTI software was used for multiple sequence alignment and phylogenetic tree construction. RESULTS: The target sequence was 1132 bp length with a 681 bpbiggest ORF encoding 226 amino acids protein with typical TSP conserved domain. It was confirmed as full length c DNA of TSP16 from Spirometra erinaceieuropaei and named as SeTSP16 (GenBank accession number: JF728872). The predicted molecular weight and isoelectric point of the deduced protein were 24 750.5 Da and 7.88 Da, respectively. Compared with TSP16s from Schistosoma japonicum and Schistosoma mansoni, it showed similarity of 59% and 59%, respectively. SeTSP16 contained four transmembrane domains (TM1-4), intracellular N and C-termini, one short small extracellular loop and one large extracellular loop. Four major epitopes that were significant different from the corresponding epitope regions of TSP16 from Schistosoma mansoni and Schistosoma japonicum were predicted. CONCLUSIONS: The full length c DNA sequences of SeTSP16 are identified. It encodes a transmembrane protein which might be an ideal diagnosis antigen and target molecule for antiparasitic drugs.

Seasonal Change of Serum Antibodies Levels of Asthma Patients and Der P1 Concentration in Dust in Their Bedrooms / 环境与健康杂志

Objective To study the seasonal change of antibodies levels in the serum of asthma patients and Der P1 concentration in the dust samples collected from their bedrooms. Methods 36 asthma patients who were allergic to mites were treated as the patients group,21 male and 15 female,aged 43 years of average age. 20 healthy individuals were treated as the control group,11 male and 9 female,with the same age as the patients group. In September and December in 2005,and Match and June in 2006,the dust samples in the bedrooms of patients and the venous blood were collected,the samples of the control were collected only in September. Der P1 concentrations in dust samples and the levels of total IgE,mite specific IgE (s-IgE),s-IgG1,s-IgG2,s-IgG3 and s-IgG4 were detected by ELISA assay. Results In autumn,the levels of total IgE,s-IgE,s-IgG1,s-IgG2,s-IgG4 and the Der P 1 concentrations in patients were significant higher compared with the control group (P