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Biochemistry ; 53(21): 3446-56, 2014 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-24801145

RESUMO

In this work, perturbed angular correlation (PAC) spectroscopy is used to study differences in the nuclear quadrupole interactions of Cd probes in DNA molecules of mice infected with the Y-strain of Trypanosoma cruzi. The possibility of investigating the local genetic alterations in DNA, which occur along generations of mice infected with T. cruzi, using hyperfine interactions obtained from PAC measurements and density functional theory (DFT) calculations in DNA bases is discussed. A comparison of DFT calculations with PAC measurements could determine the type of Cd coordination in the studied molecules. To the best of our knowledge, this is the first attempt to use DFT calculations and PAC measurements to investigate the local environment of Cd ions bound to DNA bases in mice infected with Chagas disease. The obtained results also allowed the detection of local changes occurring in the DNA molecules of different generations of mice infected with T. cruzi, opening the possibility of using this technique as a complementary tool in the characterization of complicated biological systems.


Assuntos
Cádmio/química , Doença de Chagas/genética , DNA/química , Trypanosoma cruzi , Adenina/química , Animais , Cátions Bivalentes , Complexos de Coordenação/química , Citosina/química , Guanina/química , Camundongos , Especificidade da Espécie , Timina/química
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