RESUMO
Species of the genus Psychotria are used for multiple purposes in Brazilian folk medicine, either as water infusions, baths or poultices. This study was aimed to evaluate the genotoxic and antiproliferative effects of infusions of Psychotria brachypoda and P. birotula on the Allium cepa test. Exposure to distilled water was used as a negative control, while exposure to glyphosate was used as a positive control. The interaction of extracts (as a post-treatment) with the effects of glyphosate was also studied. Results showed that glyphosate and the extracts of both P. brachypoda and P. birotula reduced the mitotic index as compared with the negative control (distilled water). Surprisingly, however, both extracts from P. brachypoda and P. birotula caused a partial reversal of the antiproliferative effect of glyphosat e when used as a post-treatment. Glyphosate also induced the highest number of cells with chromosomal alterations, which was followed by that of P. birotula extracts. However, the extracts from P. brachypoda did not show any significant genotoxic effect. Post-treatment of glyphosate-treated samples with distilled water allowed a partial recovery of the genotoxic effect of glyphosate, and some of the Psychotria extracts also did so. Notably, post-treatment of glyphosate-treated samples with P. brachypoda extracts induced a statistically significant apoptotic effect. It is concluded that P. brachypoda extracts show antiproliferative effects and are not genotoxic, while extracts of P. birotula show a less potent antiproliferative effect and may induce chromosomal abnormalities. The finding of a partial reversion of the effects of glyphosate by a post-treatment with extracts from both plants should be followed up.
Assuntos
Cebolas/citologia , Extratos Vegetais/farmacologia , Glicina/análogos & derivados , Medicina Tradicional , Plantas , Proliferação de Células , Brasil , Interações Medicamentosas , Glicina/farmacologia , Glicina/toxicidade , Inibidores Enzimáticos/farmacologia , Inibidores Enzimáticos/toxicidadeRESUMO
Species of the genus Psychotria are used for multiple purposes in Brazilian folk medicine, either as water infusions, baths or poultices. This study was aimed to evaluate the genotoxic and antiproliferative effects of infusions of Psychotria brachypoda and P. birotula on the Allium cepa test. Exposure to distilled water was used as a negative control, while exposure to glyphosate was used as a positive control. The interaction of extracts (as a post-treatment) with the effects of glyphosate was also studied. Results showed that glyphosate and the extracts of both P. brachypoda and P. birotula reduced the mitotic index as compared with the negative control (distilled water). Surprisingly, however, both extracts from P. brachypoda and P. birotula caused a partial reversal of the antiproliferative effect of glyphosat e when used as a post-treatment. Glyphosate also induced the highest number of cells with chromosomal alterations, which was followed by that of P. birotula extracts. However, the extracts from P. brachypoda did not show any significant genotoxic effect. Post-treatment of glyphosate-treated samples with distilled water allowed a partial recovery of the genotoxic effect of glyphosate, and some of the Psychotria extracts also did so. Notably, post-treatment of glyphosate-treated samples with P. brachypoda extracts induced a statistically significant apoptotic effect. It is concluded that P. brachypoda extracts show antiproliferative effects and are not genotoxic, while extracts of P. birotula show a less potent antiproliferative effect and may induce chromosomal abnormalities. The finding of a partial reversion of the effects of glyphosate by a post-treatment with extracts from both plants should be followed up.(AU)
Assuntos
Glicina/análogos & derivados , Medicina Tradicional , Proliferação de Células/efeitos dos fármacos , Cebolas/citologia , Plantas/efeitos dos fármacos , Extratos Vegetais/farmacologia , Brasil , Glicina/farmacologia , Glicina/toxicidade , Inibidores Enzimáticos/farmacologia , Inibidores Enzimáticos/toxicidade , Interações MedicamentosasRESUMO
Hospital effluents are serious problems in developing countries like Brazil, and when not treated adequately, can cause mutagenic effects on live organisms. Biomonitors, like Allium cepa L., which is one of the most used plant species when monitoring effluent genotoxicity, have been used to alert the world population about environmental contamination and genotoxic chemical emissions. The Allium cepa test was used to evaluate the genotoxicity of a hospital effluent in Santa Maria, Rio Grande do Sul State, Brazil. During the study, chromosomal disruptions, anaphasic bridges, and micronuclei during telophase were observed, indicating environmental toxicity risk.
Assuntos
Monitoramento Ambiental/métodos , Hospitais , Cebolas/efeitos dos fármacos , Esgotos/química , Bioensaio , Brasil , Divisão Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Cromossomos de Plantas/efeitos dos fármacos , Dano ao DNA , Testes de Mutagenicidade/métodos , Medição de Risco , Purificação da ÁguaRESUMO
Aloysia gratissima is used in Brazilian folk medicine for the treatment of digestive and respiratory diseases. The infusions (6 and 24 g L-1) and essential oils (0.25%, on ethanol) were prepared and we used groups of five Allium cepa bulbs for each treatment. A total of 2500 cells per treatment were analyzed and the mitotic indexes were calculated. The antiproliferative effect of infusions and essential oils of Aloysia gratissima on the Allium cepa (onion) cell cycle was evaluated using the leaves of studied specimens. The infusions presented a significant decrease in the mitotic index (4.55% at 6 g L-1 and 2.04% at 24 g L-1) compared to the control-water (6.83%), as well as for the essential oil (2.58%), in comparison to the control-ethanol (3.65%). This investigation showed that the infusions and essential oil of Aloysia gratissima present important antiproliferative effects on the Allium cepa cell cycle.