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Laryngoscope ; 131(5): 1008-1015, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33022112

RESUMO

OBJECTIVES/HYPOTHESIS: To analyze the use of highly translatable three-dimensional (3D)-printed auricular scaffolds with and without novel cartilage tissue inserts in a rodent model. STUDY DESIGN: Preclinical rodent animal model. METHODS: This prospective study assessed a single-stage 3D-printed auricular bioscaffold with or without porcine cartilage tissue inserts in an athymic rodent model. Digital Imaging and Communications in Medicine computed tomography images of a human auricle were segmented to create an external anatomic envelope filled with orthogonally interconnected spherical pores. Scaffolds with and without tissue inset sites were 3D printed by laser sintering bioresorbable polycaprolactone, then implanted subcutaneously in five rats for each group. RESULTS: Ten athymic rats were studied to a goal of 24 weeks postoperatively. Precise anatomic similarity and scaffold integrity were maintained in both scaffold conditions throughout experimentation with grossly visible tissue ingrowth and angiogenesis upon explantation. Cartilage-seeded scaffolds had relatively lower rates of nonsurgical site complications compared to unseeded scaffolds with relatively increased surgical site ulceration, though neither met statistical significance. Histology revealed robust soft tissue infiltration and vascularization in both seeded and unseeded scaffolds, and demonstrated impressive maintenance of viable cartilage in cartilage-seeded scaffolds. Radiology confirmed soft tissue infiltration in all scaffolds, and biomechanical modeling suggested amelioration of stress in scaffolds implanted with cartilage. CONCLUSIONS: A hybrid approach incorporating cartilage insets into 3D-printed bioscaffolds suggests enhanced clinical and histological outcomes. These data demonstrate the potential to integrate point-of-care tissue engineering techniques into 3D printing to generate alternatives to current reconstructive surgery techniques and avoid the demands of traditional tissue engineering. LEVEL OF EVIDENCE: NA Laryngoscope, 131:1008-1015, 2021.


Assuntos
Pavilhão Auricular/diagnóstico por imagem , Cartilagem da Orelha/cirurgia , Procedimentos de Cirurgia Plástica/efeitos adversos , Impressão Tridimensional , Infecção da Ferida Cirúrgica/epidemiologia , Alicerces Teciduais , Animais , Biópsia , Criança , Condrogênese , Desenho Assistido por Computador , Cartilagem Costal/transplante , Modelos Animais de Doenças , Pavilhão Auricular/anatomia & histologia , Pavilhão Auricular/patologia , Pavilhão Auricular/cirurgia , Cartilagem da Orelha/anatomia & histologia , Cartilagem da Orelha/diagnóstico por imagem , Cartilagem da Orelha/patologia , Humanos , Masculino , Fotografação , Poliésteres , Estudos Prospectivos , Ratos , Procedimentos de Cirurgia Plástica/instrumentação , Procedimentos de Cirurgia Plástica/métodos , Infecção da Ferida Cirúrgica/etiologia , Infecção da Ferida Cirúrgica/patologia , Infecção da Ferida Cirúrgica/prevenção & controle , Tomografia Computadorizada por Raios X , Transplante Autólogo/efeitos adversos , Transplante Autólogo/instrumentação , Resultado do Tratamento
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