Co-digestion of Theobroma cacao (Cocoa) pod husk and poultry manure for energy generation: Effects of pretreatment methods.

In this study, biogas was produced from the anaerobic co-digestion of Cocoa pod husk (CPH) and poultry manure. Pretreatment of the CPH was carried out using sulfuric acid and hydrogen peroxide. The physicochemical, elemental and structural analyses were carried out on the CPH before and after pretreatment. The microbial composition of the fermenting materials were also determined using standard method while the Fourier Transform Infra-red (FTIR) spectroscopy was used to identify the structural changes that took place after pretreatments. Use of alkaline hydrogen peroxide caused high solubilization of the lignin component of the CPH and reduced up to 81% of lignin i.e. initial value of 21.7% m.m-1 to final value of 4.2% m.m-1. Similarly, the alkali reduced the hemicellulose content of the CPH from 27.0% m.m-1 to 8.5% m.m-1. Overall, there was 68% increase in biogas volume from the alkaline pretreated CPH.

Effects of experimental Trypanosoma evansi infection on pregnancy in Yankasa ewes.

Twenty pregnant Yankasa ewes were assigned to three groups to determine the effect of Trypanosoma evansi infection on pregnancy. Groups A and B comprising seven ewes each were infected with approximately 1.0 × 10(6) cells of T evansi per ewe through venepuncture at the second and third trimesters of pregnancy, respectively. Group C comprising six ewes served as uninfected control. There was slight pyrexia in the infected groups (groups A and B) but was absent in group C. The mean body weight, glucose concentration, and packed cell volume of ewes in group A were not significantly different from those in group C throughout the study. There was also no significant difference in mean glucose concentration between groups B and C. However, in group B, mean body weight was significantly (P < 0.05) lower compared to group C at week 2 and from week 4 post infection (pi) till the end of the study; the packed cell volume also significantly (P < 0.05) decreased but at weeks 4 and 6 pi. The mean plasma protein of ewes in group A was significantly (P < 0.05) increased compared to those of group C at weeks 7, 11 pi and thereafter till the end of the study. On the contrary, the plasma protein of ewes in group B decreased significantly (P < 0.05) compared to those in group C at weeks 2 and 6 pi. There were no reproductive losses throughout the study. This was characterized by insignificant differences in the gestation length between ewes in the infected groups (groups A and B) compared with those in group C. However, there were significant (P < 0.05) decreases in lamb birth weights of ewes in group B compared with ewes in groups A and C. Mice inoculation with blood from infected ewes postpartum was parasitemic 18 to 25 days pi, for ewes in group B, whereas none of the mice in groups A and C were parasitemic. Lambs born from the infected groups (groups A and B) were also aparasitemic for 40 days postpartum. It was therefore concluded that the T evansi isolate used caused mild trypanosomosis when infected at third trimester, whereas ewes infected at second trimester were resistant.