The genus Elaphomyces (Ascomycota, Eurotiales): a ribosomal DNA-based phylogeny and revised systematics of European 'deer truffles'.

Elaphomyces ('deer truffles') is one of the most important ectomycorrhizal fungal genera in temperate and subarctic forest ecosystems, but also one of the least documented in public databases. The current systematics are mainly based on macromorphology, and is not significantly different from that proposed by Vittadini (1831). Within the 49 species recognised worldwide, 23 were originally described from Europe and 17 of these were described before the 20th century. Moreover, very recent phylogenetic treatments of the genus are mainly based on a few extra-European species and most common European species are still poorly documented. Based on an extensive taxonomic sampling mainly made in the biogeographically rich Cantabrian area (Spain), complemented with collections from France, Greece, Italy, Norway, Portugal and Sweden, all currently recognized species in Europe have been sequenced at the ITS and 28S of the rDNA. Combined phylogenetic analyses yielded molecular support to sections Elaphomyces and Ceratogaster (here emended), while a third, basal lineage encompasses the sections Malacodermei and Ascoscleroderma as well as the tropical genus Pseudotulostoma. Species limits are discussed and some taxa formerly proposed as genuine species based on morphology and biogeography are re-evaluated as varieties or forms. Spore size and ornamentation, features of the peridial surface, structure of the peridium, and the presence of mycelium patches attached to the peridial surface emerge as the most significant systematic characters. Four new species: E. barrioi, E. quercicola, E. roseolus and E. violaceoniger, one new variety: E. papillatus var. sulphureopallidus, and two new forms: E. granulatus forma pallidosporus and E. anthracinus forma talosporus are introduced, as well as four new combinations in the genus: E. muricatus var. reticulatus, E. muricatus var. variegatus, E. papillatus var. striatosporus and E. morettii var. cantabricus. Lectotypes and epitypes are designated for most recognised species. For systematic purposes, new infrageneric taxa are introduced: E. sect. Ascoscleroderma stat. nov., E. subsect. Sclerodermei stat. nov., E. subsect. Maculati subsect. nov., E. subsect. Muricati subsect. nov., and E. subsect. Papillati subsect. nov. Lastly, E.laevigatus, E. sapidus, E. sulphureopallidus and E. trappei are excluded from the genus and referred to Rhizopogon roseolus, Astraeus sapidus comb. nov., Astraeus hygrometricus and Terfezia trappei comb. nov. (syn.: Terfezia cistophila), respectively.

The Role of Polyphenols, ß-Carotene, and Lycopene in the Antioxidative Action of the Extracts of Dried, Edible Mushrooms.

One of the nutritional benefits of mushrooms is the presence of bioactive secondary metabolites which have been reported to exert various beneficial effects in vivo. Therefore, we selected thirteen frequently consumed species of Polish mushrooms and determined the concentration of polyphenols, flavonoids, ß-carotene, and lycopene in aqueous and methanolic extracts of dried fruiting bodies as well as their reducing power and ability to scavenge ABTS cation radical. We found that the concentration of antioxidants is different in different species and in various parts of the fruiting body of mushrooms. We observed a strong correlation (r > 0.9) between the concentration of total phenolics and reducing power/scavenging effects in both aqueous and methanolic extracts, while this correlation was moderate for flavonoids. Beta-carotene did not contribute discernibly to the antioxidative properties of the extracts, while lycopene had a significant contribution to the scavenging activity of methanolic mushroom extracts.

Coagulase-negative variants of methicillin-resistant Staphylococcus aureus subsp. aureus strains isolated from hospital specimens.

At the Department of Clinical Bacteriology of Medical University of Warsaw, S. aureus strains displaying negative reactions in the coagulase tube-test were isolated with a relatively high frequency from clinical specimens. As many as seventeen of such strains all from different patients were isolated during 1994 among MRSA strains. Despite coagulase negativity all strains were positive in tests for clumping factor (CF), protein A and thermonuclease. Biochemical characteristics, phage patterns and antibiotic resistance characteristics of these strains were tested. Most of the coagulase-negative strains possessed a heterogenous type of methicillin resistance. Apart from methicillin most of them were resistant to many other antimicrobials. All were resistant to gentamicin, tetracyclines and macrolide-lincosamide-streptogramine B. An occurrence of coagulase-negative S. aureus strains may lead to problems in their identification and the necessity of an application of other methods like CF, protein A or biochemical reactions.

[Selected properties of Staphylococcus aureus strains using phenotype to show a lack of coagulase synthesis]. / Wybrane wlasciwosci szczepów Staphylococcus aureus fenotypowo wykazujacych brak syntezy koagulazy.

Eighteen of S. aureus strains isolated from clinical specimens and showing negative reaction for coagulase in conventional tube test were characterized by using five different methods: ID32 Staph, API Staph, Gpl-15, STAPH-ZYM (Rosco) and classical tube methods. The membership of all examined strains to S. aureus subsp. aureus was confirmed. None of the strains produced detectable amounts of coagulase but all of them produced protein A and clumping factor. All of them were typable with phages of the III lytic group and 11 of them possessed phage pattern characteristic of the 83A complex. All the investigated hospital strains were resistant to methicillin. Five showed homogenous and 13 heterogenous type of methicillin resistance. Moreover, all of them were resistant to tetracycline, gentamicin, tobramycin and kanamycin and to MLS group of antibiotics.

[Evaluation of external laboratory test results for the correct identification and determination of chemotherapeutic sensitivity of staphylococci in bacteriologic provincial laboratories of sanitary-epidemiologic stations in 1995]. / Ocena wyników zewnatrzlaboratoryjnego sprawdzianu poprawnosci identyfikowania i oznaczania wrazliwosci gronkowców na chemioterapeutyki w bakteriologicznych pracowniach wojewódzkich Stacji Sanitarno-Epidemiologicznych w 1995 roku.

In the tests bacteriological laboratories participated in 45 sanitary-epidemiological stations. Each station was given two strains of staphylococci: S. epidermidis and S. aureus homogeneously resistant to methicillin (MRSA) or S. aureus sensitive to methicillin (MSSA), and S. aureus coagulase-negative and clumping-factor-positive strain. The analysis was carried out of the results of control identifications of strains of the determinations of the sensitivity of the identified strains to antibiotics. Among the studied strains the greatest difficulty in identification were caused by the coagulase-negative strain of S. aureus. In the determination of the sensitivity of the control strains to chemotherapeutic agents abnormalities were found in the technique of antibiogram performing and incorrect selection of discs for antibiograms as well as erroneous interpretation of the results.