The endocannabinoid system as a therapeutic target in neuropathic pain: a review.

INTRODUCTION: This review highlights the critical role of the endocannabinoid system (ECS) in regulating neuropathic pain and explores the therapeutic potential of cannabinoids. Understanding the mechanisms of the ECS, including its receptors, endogenous ligands, and enzymatic routes, can lead to innovative treatments for chronic pain, offering more effective therapies for neuropathic conditions. This review bridges the gap between preclinical studies and clinical applications by emphasizing ECS modulation for better pain management outcomes. AREAS COVERED: A review mapped the existing literature on neuropathic pain and the effects of modulating the ECS using natural and synthetic cannabinoids. This analysis examined ECS components and their alterations in neuropathic pain, highlighting the peripheral, spinal, and supraspinal mechanisms. This review aimed to provide a thorough understanding of the therapeutic potential of cannabinoids in the management of neuropathic pain. EXPERT OPINION: Advances in cannabinoid research have shown significant potential for the management of chronic neuropathic pain. The study emphasizes the need for high-quality clinical trials and collaborative efforts among researchers, clinicians, and regulatory bodies to ensure safe and effective integration of cannabinoids into pain management protocols. Understanding the mechanisms and optimizing cannabinoid formulations and delivery methods are crucial for enhancing therapeutic outcomes.

Effect of Storage Conditions and Time on the Dimensional Stability of 3D Printed Surgical Guides: An In Vitro Study.

PURPOSE: To evaluate the dimensional stability over time of additively manufactured surgical templates, fabricated by different resins, and stored by different methods. MATERIALS AND METHODS: Using a 3D printer with DLS technology and two different resins (Surgical Guide (SG)-WhipMix and Key Guide (KG)-KeystoneIndustries), 96 surgical guides were additively manufactured. The guides were stored in three different environments: directly exposed to sunlight (S1), in normal interior room conditions (S2), and in darkness (S3). The guides were digitally scanned immediately after fabrication and post-processing, and after 1, 3, and 6 months of storage. For each group, the mean deviation of the root mean square (RMS) between guide's intaglio surface, as well as the axial deviation between sleeves' housings were calculated. RESULTS: The mean axial variations of angular axis deviation of sleeves' housings ranged between 0.09° and 3.99°. The mean deviation of the RMS discrepancy in guide's intaglio ranged from 0.1 to 0.18 mm. Variations were significant (p < 0.001) only for the S1 group and only for SG material. After 3 months, an additional storage time of 3 months did not have any further effect on dimensional stability. CONCLUSIONS: Within the limitations of the present study, storage time of a surgical guide for up to 3 months after manufacturing, as well as printing material can significantly affect surgical guide's dimensional stability, when they are exposed to direct or indirect sunlight conditions. Storage of guides in a dark environment is recommended in order to avoid an additional source of error in computer-guided surgery workflows.

Transcutaneous Tibial Nerve Stimulation for Pain Management in Women with Primary Dysmenorrhea: A Randomized Clinical Trial.

Primary dysmenorrhea is considered one of the main causes of pelvic pain during a woman's childbearing years, resulting in poor quality of life. The objective was to evaluate the effectiveness of transcutaneous tibial nerve stimulation (TTNS) in painful symptomatology improvement and non-steroidal anti-inflammatory drug (NSAID) intake reduction in women with primary dysmenorrhea (PD) compared with a control group in the short, medium, and long terms. A single-blind, controlled clinical trial was developed. Participants were randomized to the experimental (TTNS) and control group (sham TTNS). Both groups received 12-weekly 30-min sessions with a NeuroTracTM PelviTone electrostimulation device. The intensity and severity of pain and non-steroidal anti-inflammatory drug (NSAID) intake were evaluated in the short-term (after treatment), medium-term (1-3 months), and long-term (6 months). A total of 61 participants were randomized, with a split of 31 (experimental group) and 30 (control group), but 55 participants completed the study and were analyzed. Statistically significant differences between both groups in the maximum pain intensity decrease (F = 4.88, p = 0.0043) measured with the visual analogue scale, as well as NSAID intake decrease (F = 4.68, p = 0.011) and days of their ingestion (F = 4.57, p = 0.012) occurred in the short term. Furthermore, significant decreases in the total number of NSAIDs ingested during the cycle (F = 3.82, p = 0.011) and the number of days on which patients ingested NSAIDs (F = 3.59, p = 0.015) in the medium-long term occurred. TTNS could be an effective and safe strategy to reduce pain caused by PD, which could reduce or complement the use of pharmacological techniques and other more invasive methods.

Effect of Hydrogen Peroxide-Based Bleaching Agents on the Color Dimensions and Surface Roughness of Different Milled Restorative Dental Materials.

PURPOSE: To compare the color dimensions, color discrepancies (ΔE00), and surface roughness of milled materials before and after application of a bleaching agent. MATERIALS AND METHODS: A total of 10 extracted molars were obtained. Each tooth was cut in transverse sections to create disks (3-mm thick, 10-mm diameter; control group). Disk specimens of eight materials (n = 10 per group) were fabricated: polymethyl methacrylate (PMMA) interim material (PMMA-Telio group), two resin nanoceramics (RNC-Ultimate group and RNC-Cerasmart group), two hybrid ceramics (HC-Shofu group and HC-Enamic group), lithium disilicate (LD-Emax group), zirconia-reinforced glass ceramic (ZGC-Suprinity group), and zirconia (Zr-InCeram group). Color measurements were obtained using a spectrophotometer before and after applying 35% hydrogen peroxide-based bleaching agent. Pre- and postbleaching surface roughness (Sa) analyses were completed using a profilometer. RESULTS: Significant L*, a*, b*, and ΔE00 value differences were found (P < .05). Color discrepancies (ΔE00) ranged from 0.30 ± 0.14 to 4.82 ± 0.10. The highest color discrepancies were measured on the PMMA-Telio group, while the lowest color discrepancies were computed for ZGC-Suprinity, RNCUltimate, and RNC-Cerasmart. Significant surface roughness differences were found (P < .05). The largest increase of surface roughness values between the pre- and postbleaching measurements was obtained in the PMMA-Telio group with a mean ΔSa value of 4.73 ± 3.02, while the largest decrease of surface roughness values between the pre- and postbleaching measurements was obtained in the Zr-InCeram group with a mean ΔSa value of -1.58 ± 0.10. CONCLUSIONS: The milled materials showed significant pre- and postbleaching color and surface roughness discrepancies.

Digital analysis of fabrication accuracy and fit in additively and subtractively manufactured implant-supported fixed complete dentures.

OBJECTIVES: To digitally evaluate the trueness and fit of additively and subtractively manufactured fixed complete dentures in materials intended for definitive use. METHODS: An edentulous maxillary model with implants at the left first molar, left canine, right canine, and right first molar site was digitized and a fixed complete denture was designed. This design was used to fabricate fixed dentures in an additively manufactured resin for definitive use (AM), a high-impact polymer composite (SM-CR), and a strength gradient zirconia (SM-ZR) (n = 10). Each fixed denture was digitized and the surface (overall, occlusal, except occlusal, and abutments), linear, and interimplant distance deviations were analyzed. The fit was assessed with the triple-scan protocol. Data were analyzed with Welch analysis of variance and Games-Howell tests (α = 0.05). RESULTS: SM-ZR led to lower overall deviations than AM, which had the highest occlusal and the lowest abutments deviations (P ≤ 0.007). SM-ZR had the lowest occlusal and SM-CR had the highest except occlusal deviations (P ≤ 0.002). AM mostly had higher linear and SM-CR mostly had higher interimplant distance deviations (P ≤ 0.043). AM led to the highest marginal gap at the left canine site, while SM-CR had the highest and SM-ZR had the lowest gaps at the right canine site (P ≤ 0.022). CONCLUSIONS: SM-ZR dentures mostly had trueness and marginal fit similar to or better than the other groups. Tested fixed complete dentures were mostly smaller than the design file in terms of interimplant distances.

Classification of Complete-Arch Implant Scanning Techniques Recorded by Using Intraoral Scanners.

OBJECTIVES: To classify the complete-arch implant scanning techniques recorded by using intraoral scanners (IOSs). OVERVIEW: Different implant scanning techniques have been described for recording complete-arch implant scans by using IOSs. However, dental literature lacks on a classification of these implant scanning techniques. Implant scanning techniques aim is to record the 3-dimensional position of the implants being scanned, while implant scanning workflows require additional scans to record all the information needed for designing an implant prosthesis. This additional information includes soft tissue information, tooth position, antagonist arch, and maxillomandibular relationship. CONCLUSIONS: There are five complete-arch implant scanning techniques captured by using IOSs: non-splinting, non-calibrated splinting, calibrated implant scan bodies, calibrated frameworks, and reverse impression methods. The digital workflow varies depending on the implant scanning technique selected. CLINICAL SIGNIFICANCE: The understanding of the varying implant scanning techniques and the main differences among them may ease the decision criteria for recording digital implant scans by using intraoral scanners.

Can nonhazardous postprocessing cleaning solutions enable adequate surface properties for printed dental casts in different resins?

STATEMENT OF PROBLEM: Printed casts and dental devices and prostheses are increasingly being used, and the ecological impact of additive manufacturing should be considered in addition to the fabrication accuracy and surface properties of the printed object. To overcome the ecological drawbacks of alcohol postprocessing, water-washable, 3-dimensionally (3D) printable cast resins and postprocessing cleaning solutions that do not include alcohol have been introduced. However, whether using only water rather than chemical solvents would enable the surface smoothness and hardness required for accurate diagnostic and prosthetic procedures is unknown. PURPOSE: The purpose of this in vitro study was to evaluate the effect of resin type (water-washable or nonwater washable) and postprocessing cleaning solution on the surface roughness and microhardness of 3D printed dental cast resins. MATERIAL AND METHODS: One hundred eight disk-shaped specimens (Ø10×2 mm) were additively manufactured (AM) from 3 dental cast resins: 2 water-washable (Epax (WW1) and Phrozen (WW2)) and 1 nonwater-washable resin (KeyModel Ultra resin-beige (NWW)) with a printer (n=36). Specimens in each resin type were divided into 3 groups for the application of postprocessing cleaning solution (water, 98% isopropyl alcohol [IPA] or methyl ether solvent) and polymerized after cleaning. The surface roughness (Ra, µm) and Vickers microhardness (HV) were measured. Laser microscope images were made of 1 specimen from each group. RESULTS: NWW-IPA (control group) had a similar Ra to WW2-water (P=.81) and WW2-methyl ether solvent (P=.511). NWW-IPA had lower HV than WW2-water (P<.001) and WW1-methyl ether solvent (P=.001). Solutions had no significant effect on the Ra of WW1 (P≥.554) and WW2 (P≥.805). WW1 had higher surface irregularities with water, whereas no significant difference was visually observed with IPA or methyl ether solvent. Solutions had a similar effect on the surface of WW2 when evaluated visually with the laser microscope. CONCLUSIONS: Resin type and postprocessing cleaning solution affected the surface roughness and microhardness of 3D printed dental cast resins, except for the surface roughness of tested water-washable resins. Water or methyl ether solvent cleaned water-washable resin (WW2) had surface roughness and hardness similar to commonly used nonwater-washable cast resin.

Influence of scanning pattern on accuracy, time, and number of photograms of complete-arch implant scans: A clinical study.

OBJECTIVES: To measure the influence of scanning pattern on the accuracy, time, and number of photograms of complete-arch intraoral implant scans. METHODS: A maxillary edentulous patient with 7 implants was selected. The reference implant cast was obtained using conventional methods (7Series Scanner). Four groups were created based on the scanning pattern used to acquire the complete-arch implant scans by using an intraoral scanner (IOS) (Trios4): manufacturer's recommended (Occlusal-Buccal-Lingual (OBL)), zig-zag (Zig-zag), circumferential (Circumf), and novel pattern that included locking an initial occlusal scan (O-Lock group) (n = 15). Scanning time and number of photograms were recorded. The linear and angular measurements were used to assess scanning accuracy. One-way ANOVA and Tukey tests were used to analyze trueness, scanning time, and number of photograms. The Levene test was selected to assess precision (α=0.05). RESULTS: Statistically significant differences in trueness were detected among OBL, Zig-zag, Circumf, and O-Lock regarding linear discrepancy (P<0.01), angular discrepancy (P<0.01), scanning time (P<0.01), and number of photograms (P<0.01). The O-Lock (63 ± 20 µm) showed the best linear trueness with statistically significant differences (P < 0.01) with Circumferential (86 ± 16 µm) and OBL (87 ± 19 µm) groups. The O-Lock (93.5 ± 13.4 s, 1080 ± 104 photograms) and Circumf groups (102.9 ± 15.1 s, 1112 ± 179 photograms) obtained lower scanning times (P < 0.01) and number of photograms (P < 0.01) than OBL (130.3 ± 19.4 s, 1293 ± 161 photograms) and Zig-zag (125.7 ± 22.1 s, 1316 ± 160 photograms) groups. CONCLUSIONS: The scanning patterns tested influenced scanning accuracy, time, and number of photograms of the complete-arch scans obtained by using the IOS tested. The zig-zag and O-Lock scanning patterns are recommended to obtain complete-arch implant scans when using the selected IOS.

Discrepancies of centric occlusion located by using a conventional method and four intraoral scanners combined with a computer-aided design program: A pilot investigation.

STATEMENT OF PROBLEM: Intraoral scanners (IOSs) can be used to record the maxillomandibular relationship at centric relation (CR). The articulated digital scans can be imported into a dental computer-aided design (CAD) program and used to locate centric occlusion (CO); however, the accuracy of the CO recorded by using IOSs and a dental CAD program remains unknown. PURPOSE: The purpose of this clinical study was to compare the position of the CO located by using a conventional method and 4 IOSs combined with a dental CAD program. MATERIAL AND METHODS: A patient volunteered to participate in this study. Conventional diagnostic stone casts were obtained. A facebow record (Kois Dentofacial Analyzer) was used to transfer the maxillary cast into a semi-adjustable articulator (Panadent PCH Articulator). A Kois deprogrammer (KD) was used to record the maxillomandibular relationship at CR and to transfer the mandibular cast into the articulator. Afterwards, CO was located in the articulated casts by removing the incisal pin and using an 8-µm articulating foil. CO was marked in the casts by using a blue articulating paper (control). Three groups were created based on the IOS used: TRIOS 4, iTero Element 5D Plus, i700, and Primescan. In each IOS group, a maxillary and mandibular scan were obtained. The scans were duplicated 10 times. Afterwards, a bilateral occlusal record captured with the KD was used to articulate each pair of duplicated scans. Each articulated specimen was imported into a CAD program (DentalCAD) and CO was virtually located. The teeth contacting at the CO of each specimen were compared with the control group. Categorical data were analyzed by using the chi-squared test (α=.05). RESULTS: The chi-squared test revealed a significant association between the IOS system and the location of the CO (P=.004). The highest association was found between the TRIOS 4 and CO position, in which 100% of the specimens obtained the same CO position as in the conventional group. The lowest association was found between the i700 and CO position. In the i700 group, 20% of the specimens showed the same CO position as in the control group. A similar outcome was obtained in the iTero and Primescan groups. In both groups, 60% of the specimens demonstrated the same CO position as the control group. CONCLUSIONS: The IOS system used to acquire articulated scans at CR impacted the CO position located by using the evaluated digital methods. The TRIOS 4 system was the only IOS that consistently reproduced the same CO position as the conventional method.

Fracture resistance, marginal and internal adaptation of innovative 3D-printed graded structure crown using a 3D jet printing technology.

PURPOSE: This in vitro study aimed to create a graded structured dental crown using 3D printing technology and investigate the fracture resistance and the adaptation of this new design. MATERIALS AND METHODS: A dental crown with a uniform thickness of 1.5 mm was designed, and the exported stereolithography file (STL) was used to manufacture 30 crowns in three groups (n = 10), solid (SC), bilayer (BL), and multilayer (ML) crowns using  3D jet printing technology. Marginal and internal gaps were measured using the silicone replica technique. Crowns were then luted to a resin die using a temporary luting agent and the fracture resistance was measured using a universal testing machine. One-way ANOVA and Tukey post hoc tests were used to compare the fracture resistance and the adaptation of crowns at a significance level of 0.05. RESULTS: Mean marginal and internal gap of the ML group were 80 and 82 mm, respectively; which were significantly (p < 0.05) smaller than BL (203 and 183 mm) and SC (318 and 221 mm) groups. The SC group showed the highest mean load at fracture (2330 N) which was significantly (p < 0.05) higher than the BL (1716 N) and ML (1516 N) groups. CONCLUSION: 3D jet printing technology provides an opportunity to manufacture crowns in a graded structure with various mechanical properties. This study provided an example of graded structured crowns and presented their fracture resistance. SC group had the highest fracture resistance; however, ML had the best marginal and internal adaptation.

Pharmacological Treatment for Dialysis-Related Muscle Cramps: A Systematic Review.

BACKGROUND: Patients with end-stage renal disease undergoing dialysis suffer from muscle cramps, a prevalent and burdensome symptom for which there is a paucity of efficient and safe treatments. AIM: What is the efficacy and safety of pharmacological interventions for the treatment of dialysis-related muscle cramps? DESIGN: A systematic review was conducted in OVID, CINAHL, PubMed, Web of Science, and Central Cochrane databases up to August 25, 2023. DATA SOURCES: Experimental studies reporting on a pharmacological intervention for the treatment of dialysis-related muscle cramps were included. The review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analysis, and the studies quality was assessed with the RoB2 tool. RESULTS: A total of 4660 studies were retrieved, and 13 articles were included. The studies reported on nine interventions: vitamin C, vitamin E, vitamin K2, vitamin B7, dextrose solutions, gabapentin, sodium chloride, creatine monohydrate, and L-carnitine. The studies testing L-carnitine and creatine monohydrate were the only ones deemed to have a low risk of bias. Side effects were reported in only two trials, consisting primarily of gastrointestinal discomfort and hyperglycemia. Vitamins C and E are the two most studied interventions that showed positive results in reducing the frequency, severity, and duration of dialysis-related muscle cramps. L-carnitine is a promising intervention that warrants further investigation. CONCLUSION: Our review consolidates the existing evidence, elucidating the range of treatments along with their potential benefits and limitations. Future studies should uphold high-quality standards, incorporate patient-reported outcomes, and utilize well-defined, robust samples to improve patient care.

Influence of apical finish line location of tooth preparations on the scanning accuracy of intraoral scanners with various focal lengths and scanning technologies.

STATEMENT OF PROBLEM: Limited studies have reported the influence of finish line location on the accuracy of intraoral scanners (IOSs). Focal length is a hardware characteristic of IOSs. Whether there is a relationship between scanning accuracy of tooth preparations with the finish located at different apical positions and focal length and IOS technology or system remains uncertain. PURPOSE: The purpose of the present in vitro study was to assess the influence of the apical finish line location of tooth preparations on the accuracy of 4 IOSs with various focal lengths and scanning technologies. MATERIAL AND METHODS: A maxillary typodont with a crown preparation on the left first molar was digitized (T710). Afterwards, a removable die was created on the prepared first molar of the virtual cast and duplicated to create 4 dies with different apical finish line locations: 2- or 1-mm supragingival, 0-mm or equigingival, and -0.5-mm or intracrevicular. The cast and die designs were additively fabricated (Asiga Pro 4K with Keystone Model Ultra). Each die was independently scanned by using the same laboratory scanner (reference scans). Four groups were created: TRIOS 5, i700, iTero, and Primescan. Four subgroups were developed depending on the apical position of the finish line (n=15). In each subgroup, the cast was assembled by positioning the corresponding die into the cast. The cast was then scanned by using the corresponding IOS. The reference scans were used as a control to compute the root mean square (RMS) error discrepancies with each experimental scan on the preparation and margin of the preparation areas. Two-way ANOVA and pairwise comparisons were used to analyze trueness (α=.05). The Levene and pairwise comparisons using the Wilcoxon Rank sum test were used to analyze precision (α=.05). RESULTS: Trueness discrepancies in the preparation area were found among the groups (P=.010) and subgroups (P<.001), with a significant interaction between group×subgroup (P<.001). The -0.5 mm location obtained significantly worse trueness in the preparation area. The TRIOS 5 and i700 obtained the best trueness in the preparation area. Trueness discrepancies in the margin area were found among the groups (P=.002) and subgroups (P<.001), with a significant interaction between group×subgroup (P=.004). The -0.5 mm location obtained the worst trueness in the margin area. The i700 and Primescan obtained the best trueness in the margin area. Precision discrepancies were found in the preparation area (P<.001). The TRIOS 5 obtained the best precision in the preparation area (P=.001). Precision discrepancies in the margin area were obtained (P<.001). The 1-mm subgroup obtained the best precision (P=.001). CONCLUSIONS: The apical position of the finish line of the tooth preparation tested affected the trueness and precision of the IOSs tested.

True horizontal or gravity plane registration for transferring the maxillary scan into the virtual articulator by using a facial scanner without the need for an additional device.

Different reference planes can be used to transfer the maxillary cast into the analog articulator, including the true horizontal or gravity reference plane. Different techniques have been described to record the gravity reference plane for transferring the maxillary scan into the virtual articulator by using facial scanning techniques. However, these digital facebow procedures require the use of an extraoral scan body system, printed reference device, or orientation reference board. This manuscript describes a technique for recording the gravity reference plane by using a facial scanner without the use of an additional device. This technique aims to reduce the clinical time needed to capture a patient's digital data and minimize the laboratory time needed to integrate the virtual patient and transfer the maxillary scan into the virtual articulator.

Remodeling p38 signaling in muscle controls locomotor activity via IL-15.

Skeletal muscle has gained recognition as an endocrine organ releasing myokines upon contraction during physical exercise. These myokines exert both local and pleiotropic health benefits, underscoring the crucial role of muscle function in countering obesity and contributing to the overall positive effects of exercise on health. Here, we found that exercise activates muscle p38γ, increasing locomotor activity through the secretion of interleukin-15 (IL-15). IL-15 signals in the motor cortex, stimulating locomotor activity. This activation of muscle p38γ, leading to an increase locomotor activity, plays a crucial role in reducing the risk of diabetes and liver steatosis, unveiling a vital muscle-brain communication pathway with profound clinical implications. The correlation between p38γ activation in human muscle during acute exercise and increased blood IL-15 levels highlights the potential therapeutic relevance of this pathway in treating obesity and metabolic diseases. These findings provide valuable insights into the molecular basis of exercise-induced myokine responses promoting physical activity.

Chronic neuropathic pain components in whiplash-associated disorders correlate with metabolite concentrations in the anterior cingulate and dorsolateral prefrontal cortex: a consensus-driven MRS re-examination.

Introduction: Whiplash injury (WHI) is characterised by a forced neck flexion/extension, which frequently occurs after motor vehicle collisions. Previous studies characterising differences in brain metabolite concentrations and correlations with neuropathic pain (NP) components with chronic whiplash-associated disorders (WAD) have been demonstrated in affective pain-processing areas such as the anterior cingulate cortex (ACC). However, the detection of a difference in metabolite concentrations within these cortical areas with chronic WAD pain has been elusive. In this study, single-voxel magnetic resonance spectroscopy (MRS), following the latest MRSinMRS consensus group guidelines, was performed in the anterior cingulate cortex (ACC), left dorsolateral prefrontal cortex (DLPFC), and occipital cortex (OCC) to quantify differences in metabolite concentrations in individuals with chronic WAD with or without neuropathic pain (NP) components. Materials and methods: Healthy individuals (n = 29) and participants with chronic WAD (n = 29) were screened with the Douleur Neuropathique 4 Questionnaire (DN4) and divided into groups without (WAD-noNP, n = 15) or with NP components (WAD-NP, n = 14). Metabolites were quantified with LCModel following a single session in a 3 T MRI scanner within the ACC, DLPFC, and OCC. Results: Participants with WAD-NP presented moderate pain intensity and interference compared with the WAD-noNP group. Single-voxel MRS analysis demonstrated a higher glutamate concentration in the ACC and lower total choline (tCho) in the DLPFC in the WAD-NP versus WAD-noNP group, with no intergroup metabolite difference detected in the OCC. Best fit and stepwise multiple regression revealed that the normalised ACC glutamate/total creatine (tCr) (p = 0.01), DLPFC n-acetyl-aspartate (NAA)/tCr (p = 0.001), and DLPFC tCho/tCr levels (p = 0.02) predicted NP components in the WAD-NP group (ACC r 2 = 0.26, α = 0.81; DLPFC r 2 = 0.62, α = 0.98). The normalised Glu/tCr concentration was higher in the healthy than the WAD-noNP group within the ACC (p < 0.05), but not in the DLPFC or OCC. Neither sex nor age affected key normalised metabolite concentrations related to WAD-NP components when compared to the WAD-noNP group. Discussion: This study demonstrates that elevated glutamate concentrations within the ACC are related to chronic WAD-NP components, while higher NAA and lower tCho metabolite levels suggest a role for increased neuronal-glial signalling and cell membrane dysfunction in individuals with chronic WAD-NP components.

Digital workflow for the fabrication of custom-fit additively manufactured sports mouthguards with balanced occlusion using an optical jaw tracking system: A dental technique.

Custom sports mouthguards are used in various sports to protect teeth, temporomandibular joints, and soft tissues from impact forces. The present article demonstrates a digital workflow to fabricate a 3-dimensionally (3D) printed individualized sports mouthguard. An optical jaw tracking system is used to record a repeatable reference position, and mandibular excursive movements to achieve a completely balanced occlusion. The technique simplifies the fabrication of a custom-fit mouthguard over the conventional approach by providing increased thickness accuracy, control of design, and integration of jaw motion.

Intraoral scanners as tracking devices: A dental protocol for assessing volumetric changes between intraoral scans.

Intraoral scanners (IOSs) are digital data acquisition technologies that ease the recording of virtual diagnostic casts. Some IOSs have a specific software tool to assess volumetric changes between 2 scans acquired on the patient at different times. The scans are superimposed and volumetric differences between both meshes are reported. However, these software tools may be limited to scans captured only by the IOS of the same manufacturer. The present manuscript describes a protocol for comparing volumetric changes between 2 scans recorded using any IOS. Additionally, 1 of the scans is divided into 3 sections to minimize the alignment distortion and maximize the evaluation of the volumetric changes.

Implant-supported prostheses following the biologically oriented preparation technique (BOPT) after implantoplasty procedures: A dental technique.

Esthetic concerns may appear on implant-supported prostheses after peri-implantitis treatment, such as implantoplasty procedures that includes the thread removal and surface smoothening. A technique for restoring implants that have been treated for peri-implantitis using an implantoplasty procedure combined with the detoxification of the implant surfaces is described. The technique involves the fabrication of an implant-supported prosthesis following the biologically oriented preparation technique (BOPT) and aims to solve esthetic complications after this peri-implantitis treatment approach.

Epidemiology of traumatic spinal cord injury in Spain: A ten-year analysis of trend of clinical and demographic characteristics.

CONTEXT: The study of epidemiological changes of traumatic spinal cord injury (TSCI) is needed due to its highly variable incidence. OBJECTIVE: To determine the incidence of TSCI in Spain and to describe the trend of clinical and demographic characteristics according to age group during a 10-year period. METHODS: A prospective cohort study was conducted. A multidisciplinary team evaluated all individuals with new TSCI. The data were recorded according to the International Spinal Cord Injury Core Data Sets. RESULTS: In a 10-year period, 933 new patients with TSCI were admitted to the hospital. The annual incidence of TSCI was 6.2 per million. The leading causes of injury were traffic accidents (38.5%), low-level falls (20.6%), and high-level falls (19.1%). Males, age group of 31-45 years, and cervical level of injury were the most common profiles of TSCI. In patients over 60 years,71.5% were injured following a fall, particularly low-level falls (47.2%). In patients under 60 years old, the leading cause of SCI was traffic accidents (46%). The proportion of tetraplegia in patients above 60 years was 68.3%, compared to 43.7% in patients under 60 years of age. Patients in the age group above 60 years were hospitalized with a shorter duration of rehabilitation compared to younger age group. CONCLUSIONS: Compared with globally estimated data reported in previous studies, this research demonstrated a low incidence of TSCI in Spain, suggesting a decrease in the last years. Falls and traffic accidents were the most common causes of TSCI in elderly and youth, respectively. Prevention programs should focus on these issues.

Accuracy of artificial intelligence-based segmentation of the mandibular canal in CBCT.

OBJECTIVES: To investigate the accuracy of artificial intelligence (AI)-based segmentation of the mandibular canal, compared to the conventional manual tracing, implementing implant planning software. MATERIALS AND METHODS: Localization of the mandibular canals was performed for 104 randomly selected patients. A localization was performed by three experienced clinicians in order to serve as control. Five tracings were performed: One from a clinician with a moderate experience with a manual tracing (I1), followed by the implementation of an automatic refinement (I2), one manual from a dental student (S1), and one from the experienced clinician, followed by an automatic refinement (E). Subsequently, two fully automatic AI-driven segmentations were performed (A1,A2). The accuracy between each method was measured using root mean square error calculation. RESULTS: The discrepancy among the models of the mandibular canals, between the experienced clinicians and each investigated method ranged from 0.21 to 7.65 mm with a mean of 3.5 mm RMS error. The analysis of each separate mandibular canal's section revealed that mean RMS error was higher in the posterior and anterior loop compared to the middle section. Regarding time efficiency, tracing by experienced users required more time compared to AI-driven segmentation. CONCLUSIONS: The experience of the clinician had a significant influence on the accuracy of mandibular canal's localization. An AI-driven segmentation of the mandibular canal constitutes a time-efficient and reliable procedure for pre-operative implant planning. Nevertheless, AI-based segmentation results should always be verified, as a subsequent manual refinement of the initial segmentation may be required to avoid clinical significant errors.