RESUMO
Seasonal influenza virus causes acute respiratory tract infections, which can be severe in children and the elderly. At present, rapid influenza diagnostic tests (RIDTs) are popular at clinical sites because they enable early diagnosis and avoid unnecessary use of antibiotics; in addition, high risk patients with underlying disease can be given antiviral drugs. However, the sensitivity and specificity of some of those tests are relatively poor. To overcome these problems, nucleic acid-based molecular point-of-care tests have been developed; however, they are significantly more expensive than RIDTs. Previously, the authors developed real-time reverse transcription loop-mediated isothermal amplification (rRT-LAMP) assays using a quenching primer to detect influenza viruses. However, the assay is limited to laboratory use because it requires a nucleic acid purification step and preparation of reaction mixtures on ice. Therefore, the authors developed and validated direct rRT-LAMP assays that require no nucleic acid purification steps using commercial RNA isolation kits, and no storage and handling of reagents on ice. These assays can be performed within 10-30 min and require only mixing a clinical specimen with extraction reagent followed by addition of a lyophilized detection reagent. The established assay showed high sensitivity and specificity when validated using 310 clinical specimens. Thus, the assay is a powerful tool for molecular diagnosis of seasonal influenza virus infection in the clinic.
Assuntos
Influenza Humana/diagnóstico , Técnicas de Diagnóstico Molecular/métodos , Técnicas de Amplificação de Ácido Nucleico/métodos , Transcrição Reversa , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Primers do DNA/genética , Feminino , Fluorescência , Humanos , Influenza Humana/virologia , Masculino , Pessoa de Meia-Idade , Técnicas de Diagnóstico Molecular/normas , RNA Viral/genética , Infecções Respiratórias/virologia , Sensibilidade e Especificidade , Adulto JovemRESUMO
A clinical trial was conducted at HCM city Traditional Medicine Hospital, on 70 patients with chronic sinusitis, chronic rhinititis using trivixomtea. The preparation exerted good effect on sinusitis. The symptoms such as head ache, stuffy has abased obviously, high rate of treatment was reported, X-ray image was improved. There is no considerable side effect