RESUMO
In this study, the effect of transition metal dopants, originally added as colouring agents, on the mechanical properties and biocompatibility of sintered zirconia was investigated. This study confirmed that transition metal dopants could have a slight detrimental effect on the mechanical properties of zirconia. The addition of metal dopants did not affect the adhesion and proliferation of gingival fibroblasts.
Assuntos
Materiais Biocompatíveis , Cerâmica , Metais/química , ZircônioRESUMO
This study examined the cell response to a TiO2 nanotubular surface (Ti-NT) for future biomedical applications. The level of cell attachment and spreading at 20 min and 60 min was evaluated by SEM. IL-6 and PGE2 secretion was evaluated by ELISA. In SEM analysis, the Ti-NT surface had more fully spread cells compared to the machined titanium surface (Ti-S). ELISA revealed that the level of IL-6 and PGE2 production was higher on the Ti-NT than on the Ti-S. These results suggest that a surface treatment with a nanotubular TiO2 surface enhances the early osteoblast responses, such as cell spreading and cytokine release, which are important for subsequent cell functions and bone healing in vivo.
Assuntos
Nanotubos/química , Crânio/efeitos dos fármacos , Titânio/farmacologia , Animais , Movimento Celular/efeitos dos fármacos , Células Cultivadas , Materiais Revestidos Biocompatíveis/química , Dinoprostona/biossíntese , Interleucina-6/biossíntese , Nanopartículas Metálicas/química , Nanopartículas Metálicas/ultraestrutura , Microscopia Eletrônica de Varredura , Nanotecnologia , Nanotubos/ultraestrutura , Osteoclastos/citologia , Osteoclastos/efeitos dos fármacos , Osteoclastos/fisiologia , Ratos , Crânio/citologia , Crânio/fisiologiaRESUMO
The biological response of fetal rat calvarial cells on a TiO2 nanotubular surface (Ti-NT) was evaluated by cell viability assay, alkaline phosphatase (ALP) activity and reverse transcription polymerase chain reaction (RT-PCR) analysis. The cell viability assay showed no significant difference between the Ti-NT and smooth titanium surfaces (Ti-S). Ti-NT had better cellular responses with regard to the ALP activity and bone-associated markers, such as bone sialoprotein and osteocalcin mRNA than Ti-S. These results suggest that Ti-NT stimulate the differentiation into osteoblasts of fetal rat calvarial cells, potentially contributing to rapid osseointegration.
Assuntos
Nanotubos/química , Nanotubos/ultraestrutura , Osteoblastos/citologia , Osteoblastos/fisiologia , Osteogênese/fisiologia , Titânio/química , Animais , Adesão Celular , Diferenciação Celular , Células Cultivadas , Substâncias Macromoleculares/química , Teste de Materiais , Conformação Molecular , Tamanho da Partícula , Ratos , Propriedades de SuperfícieRESUMO
The purpose of this study was to fabricate and characterize nanotubular structure on machined titanium (MA) and resorbable blast media (RBM) treated titanium by anodizing. The anodized MA and RBM were characterized with scanning electron microscopy, transmission electron microscopy, X-ray diffraction, energy disperse spectra, X-ray photoelectron spectra, and nano-indentation and scratch test. Highly ordered nanotubular layers of individually 100 nm in diameter and 500 nm in length approximately were formed regardless of the substrates. The nanotubular layers consisted mainly of amorphous TiO(2) with trace fluorine. The nanotubular surfaces on both the substrates significantly reduced water contact angles and elastic modulus compared with those prior to anodizing. The anodizing treatment significantly increased the surface roughness of the smooth MA, but significantly decreased the surface roughness of the roughened RBM. The critical delamination forces of the nanotubular layer were not obtained due to the limitation of surface roughness. The anodized RBM consisted of a nano-micro porous graded structure, or a nanotubular amorphous fluoride containing TiO(2) layer on top of micro-roughened titanium surface, which is expected to significantly improve the surface area that can be used to deliver drugs and growth factors and alleviate the interfacial elastic modulus mismatch as to enhance osseointegration when compared with conventional dental and orthopedic implant devices with smooth or acid etched surface.