Tacticity in chiral phononic crystals.

The study of vibrational properties in engineered periodic structures relies on the early intuitions of Haüy and Boscovich, who regarded crystals as ensembles of periodically arranged point masses interacting via attractive and repulsive forces. Contrary to electromagnetism, where mechanical properties do not couple to the wave propagation mechanism, in elasticity this paradigm inevitably leads to low stiffness and high-density materials. Recent works transcend the Haüy-Boscovich perception, proposing shaped atoms with finite size, which relaxes the link between their mass and inertia, to achieve unusual dynamic behavior at lower frequencies, leaving the stiffness unaltered. Here, we introduce the concept of tacticity in spin-spin-coupled chiral phononic crystals. This additional layer of architecture has a remarkable effect on their dispersive behavior and allows to successfully realize material variants with equal mass density and stiffness but radically different dynamic properties.

Tandem emulsification for high-throughput production of double emulsions.

Core-shell double emulsions produced using microfluidic methods with controlled structural parameters exhibit great potential in a wide range of applications, but the low production rate of microfluidic methods hinders the exploitation of the capabilities of microfluidics to produce double emulsions with well-defined features. A major obstacle towards the scaled-up production of core-shell double emulsions is the difficulty of achieving robust spatially controlled wettability in integrated microfluidic devices. Here, we use tandem emulsification, a two-step process with microfluidic devices, to scale up the production. With this method, single emulsions are generated in a first device and are re-injected directly into a second device to form uniform double emulsions. We demonstrate the application of tandem emulsification for scalable core-shell emulsion production with both integrated flow focusing and millipede devices and obtain emulsions of which over 90% are single-core monodisperse double emulsion drops. With both mechanisms, the shell thickness can be controlled, so that shells as thin as 3 µm are obtained for emulsions 50 µm in radius.

Enhanced single-cell printing by acoustophoretic cell focusing.

Recent years have witnessed a strong trend towards analysis of single-cells. To access and handle single-cells, many new tools are needed and have partly been developed. Here, we present an improved version of a single-cell printer which is able to deliver individual single cells and beads encapsulated in free-flying picoliter droplets at a single-bead efficiency of 96% and with a throughput of more than 10 beads per minute. By integration of acoustophoretic focusing, the cells could be focused in x and y direction. This way, the cells were lined-up in front of a 40 µm nozzle, where they were analyzed individually by an optical system prior to printing. In agreement with acoustic simulations, the focusing of 10 µm beads and Raji cells has been achieved with an efficiency of 99% (beads) and 86% (Raji cells) to a 40 µm wide center region in the 1 mm wide microfluidic channel. This enabled improved optical analysis and reduced bead losses. The loss of beads that ended up in the waste (because printing them as single beads arrangements could not be ensured) was reduced from 52% ± 6% to 28% ± 1%. The piezoelectric transducer employed for cell focusing could be positioned on an outer part of the device, which proves the acoustophoretic focusing to be versatile and adaptable.