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OBJECTIVE:To investigate the effects of 4 polar parts extracts from Li medicine Alpinia oxyphylla fruit on model mice with experimental colitis,and screen the effective parts of A. oxyphylla fruit in the treatment of colitis. METHODS:After 70% ethanol extract dispersed by water,petroleum ether,trichloromethane,ethyl acetate and n-butanol were used in turn to obtain extractions in related parts. 42 mice were randomly divided into modeling group(36 mice)and blank group(6 mice). Mice in mod-eling group were taken 2,4,6-nitrobenzne sulfonic acid method to replicate the experiment colitis. After modeling,model mice were randomly divided into model group,positive group(Sulfasalazine enteric coated tablets,52 g/kg),petroleum ether,trichloro-methane,ethyl acetate,n-butanol extract from A. oxyphylla fruit groups(10 g/kg,calculated by crude drug),6 in each group,in-tragastrically administrated once a day,for 9 d,0.2 mL/10 g;mice in blank group and model group were intragastrically given nor-mal saline. After administration,body mass of mice was determined,disease activity index(DAI)was scored and colonic myelo-peroxidase(MPO),superoxide dismutase(SOD),malondialdehyde(MDA)activities in colon tissue were detected,and the colon pathological changes were observed and scored. RESULTS:Compared with blank group,decrease percentage of body mass,DAI score,MPO and MDA activities in colon tissue in model group were increased(P<0.05 or P<0.01);colon tissue was pathologi-cally changed. Compared with model group,body mass was increased,DAI score,MPO activity and pathological score in each ad-ministration groups were decreased;SOD activities in positive group,n-butanol extract from A. oxyphylla fruit group and ethyl ace-tate extract from A. oxyphylla fruit group were significantly increased,MDA activities in positive group and ethyl acetate extract from A. oxyphylla fruit group were significantly decreased,with statistical significances (P<0.05 or P<0.01). CONCLUSIONS:The 4 polar parts extracs from A. oxyphylla fruit have certain improvement effects on model mice with experimental colitis, in which ethyl acetate and n-butanol parts are preferred.
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Objective To study the relationship between sperm activity and the level of L-carnitine in seminal plasma of patients with idiopathic asthenospermia by HPLC.Methods Experimental group:idiopathic asthenospermia 50 cases,and the control group:male with pregnancy history 10 cases.The semen parameters (volume,sperm density,viability,motility),and L-carnitine level in seminal plasma were detected with high performance liquid chromatography method.Results There were significant difference between A and B group about sperm density and the percentage of progressive motile spermatozoa,and the level of L-carnitine(P＜0.01).Conclusions The decrease of L-carnitine level in patients with idiopathic asthenospermia,is closely related to the low sperm motility,which shows that L-carnitine can be used to treat idiopathic asthenospermia effectively.
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Objective:To evaluate the improvement of pereutaneous epididymal sperm aspiration(PESA)and testicular sperm extraction(TESE) and the therapy value in the diagnosis and treatment of azoospermia. Methods:We examined 218 azoospermia patients by the techniques of PESA and TESE.Results:Of the total number of the azoospermia patients,94(43.12%)had sperm in the epididymis and 28(12.84%)in the testis.Intracytoplasmic sperm injection (ICSI) was applied to 16 patients with sperm in the epididymis or testis after sperm extraction.The pregnancy rate after the embryo transfer was 37.5%. Conclusion:The improvement of the PESA and TESE can provide safer chances of procreation to azoospermia patients with partial obstruction and effective therapies for azoospermia.
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To improve the class effectiveness,different teaching methods were chosen based on the characteristics of pharmacy students,such as to stimulate the students’ interest,raise the independently ability,design the appropriate multimedia content and practice.
