Predicting risk of endometrial failure: a biomarker signature that identifies a novel disruption independent of endometrial timing in patients undergoing hormonal replacement cycles.

OBJECTIVE: To propose a new gene expression signature that identifies endometrial disruptions independent of endometrial luteal phase timing and predicts if patients are at risk of endometrial failure. DESIGN: Multicentric, prospective study. SETTING: Reproductive medicine research department in a public hospital affiliated with private fertility clinics and a reproductive genetics laboratory. PATIENTS: Caucasian women (n = 281; 39.4 ± 4.8 years old with a body mass index of 22.9 ± 3.5 kg/m2) undergoing hormone replacement therapy between July 2018 and July 2021. Endometrial samples from 217 patients met RNA quality criteria for signature discovery and analysis. INTERVENTION(S): Endometrial biopsies collected in the mid-secretory phase. MAIN OUTCOME MEASURE(S): Endometrial luteal phase timing-corrected expression of 404 genes and reproductive outcomes of the first single embryo transfer (SET) after biopsy collection to identify prognostic biomarkers of endometrial failure. RESULTS: Removal of endometrial timing variation from gene expression data allowed patients to be stratified into poor (n = 137) or good (n = 49) endometrial prognosis groups on the basis of their clinical and transcriptomic profiles. Significant differences were found between endometrial prognosis groups in terms of reproductive rates: pregnancy (44.6% vs. 79.6%), live birth (25.6% vs. 77.6%), clinical miscarriage (22.2% vs. 2.6%), and biochemical miscarriage (20.4% vs. 0%). The relative risk of endometrial failure for patients predicted as a poor endometrial prognosis was 3.3 times higher than those with a good prognosis. The differences in gene expression between both profiles were proposed as a biomarker, coined the endometrial failure risk (EFR) signature. Poor prognosis profiles were characterized by 59 upregulated and 63 downregulated genes mainly involved in regulation (17.0%), metabolism (8.4%), immune response, and inflammation (7.8%). This EFR signature had a median accuracy of 0.92 (min = 0.88, max = 0.94), median sensitivity of 0.96 (min = 0.91, max = 0.98), and median specificity of 0.84 (min = 0.77, max = 0.88), positioning itself as a promising biomarker for endometrial evaluation. CONCLUSION(S): The EFR signature revealed a novel endometrial disruption, independent of endometrial luteal phase timing, present in 73.7% of patients. This EFR signature stratified patients into 2 significantly distinct and clinically relevant prognosis profiles providing opportunities for personalized therapy. Nevertheless, further validations are needed before implementing this gene signature as an artificial intelligence (AI)-based tool to reduce the risk of patients experiencing endometrial failure.

Field-scale evaluation of struvite phosphorus and nitrogen leaching relative to monoammonium phosphate.

Struvite (MgNH4 PO4 ·6H2 O) is a wastewater-derived phosphorus (P) fertilizer with potential to reduce P as well as nitrogen (N) losses due to its low water solubility. To test hypothesized lower P and N losses from struvite relative to monoammonium phosphate (MAP), field experiments with a randomized-complete block design were conducted in central (Urbana) Illinois on an Endoaquoll-Argiudoll complex and in southern (Ewing) Illinois on a Fragiudalf-Hapludalf complex. Fertilizer was broadcast applied in the fall prior to spring planting of soybean (Glycine max L.) at a maintenance rate of 29.5 kg P ha-1 (Urbana) and 22.0 kg P ha-1 (Ewing). In the spring, soil extractable N and Mehlich 3-P at 0- to 15-cm and 15- to 35-cm depths were determined, and leached N and P were estimated using fall-installed ion-exchangeable resin (IER) lysimeters. At Urbana, soil extractable nitrate-N was higher under MAP than struvite at 0- to 15-cm depth. At Ewing, soil Mehlich 3-P under struvite was lower than MAP at both depths. At Urbana, leached P was 10-fold lower, and leached N was twofold lower under struvite than MAP. Soybean yields were similar between MAP and struvite at Urbana (4.1-4.3 Mg ha-1 ) and Ewing (3.2-3.5 Mg ha-1 ), but at Ewing yields were 23% higher under struvite compared to the P-unfertilized control. Off-season yield-scaled P and N losses under struvite were lower than MAP by 51% at Urbana and by 10% at Ewing. Our results support the hypothesized potential of struvite to reduce nutrient losses while meeting crop P needs. Additionally, we identify disproportionally greater reductions in N leaching and yield-scaled N losses by substituting struvite for MAP in fall applications, indicating that struvite can offer greater relative benefits for N loss reduction than P loss reduction.

Social cognition and emotional rehabilitation in participants with schizofrenia.

Introduction: People with schizophrenia have deficits in social cognition, emotion and social perception, as well as attributional style. The purpose of this study was to evaluate the efficacy of a multicomponent social cognition training program, e-Motional Training® (ET), in people with schizophrenia and to compare its efficacy with people who did not receive it. Therefore, a single-blind RCT was conducted in participants with a diagnosis of schizophrenia. Methods: A randomized, single-blind, clinical trial was conducted with 50 stable outparticipants with schizophrenia (registry number CHUC_2019_109). All participants (control and intervention) were treated with pharmacotherapy, case management and were on Individual Placement and Support methodology for competitive employment. The intervention group was treated with ET, an online program designed for social cognition rehabilitation. Pre and post assessment was performed using different battery of tests. General mixed models with subject identification and repeated measures over time were used. Results: Different pre and post measurements were performed in the two groups. No significant differences were found in sociodemographic characteristics between the control and intervention groups. Improvements were obtained in the intervention group in the Ekman test (p = 0.009), mainly enhanced by the improvement shown in three emotions: fear, sadness and disgust (p = 0.041, p = 0.021 and p = 0.038 respectively). Conclusion: ET is a promising online training tool for social cognition deficits in schizophrenia, in particular, for the improvement of emotions.Clinical Trial Registration: https://beta.clinicaltrials.gov, NCT05866328.

Deciphering a shared transcriptomic regulation and the relative contribution of each regulator type through endometrial gene expression signatures.

BACKGORUND: While various endometrial biomarkers have been characterized at the transcriptomic and functional level, there is generally a poor overlap among studies, making it unclear to what extent their upstream regulators (e.g., ovarian hormones, transcription factors (TFs) and microRNAs (miRNAs)) realistically contribute to menstrual cycle progression and function. Unmasking the intricacies of the molecular interactions in the endometrium from a novel systemic point of view will help gain a more accurate perspective of endometrial regulation and a better explanation the molecular etiology of endometrial-factor infertility. METHODS: An in-silico analysis was carried out to identify which regulators consistently target the gene biomarkers proposed in studies related to endometrial progression and implantation failure (19 gene lists/signatures were included). The roles of these regulators, and of genes related to progesterone and estrogens, were then analysed in transcriptomic datasets compiled from samples collected throughout the menstrual cycle (n = 129), and the expression of selected TFs were prospectively validated in an independent cohort of healthy participants (n = 19). RESULTS: A total of 3,608 distinct genes from the 19 gene lists were associated with endometrial progression and implantation failure. The lists' regulation was significantly favoured by TFs (89% (17/19) of gene lists) and progesterone (47% (8 /19) of gene lists), rather than miRNAs (5% (1/19) of gene lists) or estrogen (0% (0/19) of gene lists), respectively (FDR < 0.05). Exceptionally, two gene lists that were previously associated with implantation failure and unexplained infertility were less hormone-dependent, but primarily regulated by estrogen. Although endometrial progression genes were mainly targeted by hormones rather than non-hormonal contributors (odds ratio = 91.94, FDR < 0.05), we identified 311 TFs and 595 miRNAs not previously associated with ovarian hormones. We highlight CTCF, GATA6, hsa-miR-15a-5p, hsa-miR-218-5p, hsa-miR-107, hsa-miR-103a-3p, and hsa-miR-128-3p, as overlapping novel master regulators of endometrial function. The gene expression changes of selected regulators throughout the menstrual cycle (FDR < 0.05), dually validated in-silico and through endometrial biopsies, corroborated their potential regulatory roles in the endometrium. CONCLUSIONS: This study revealed novel hormonal and non-hormonal regulators and their relative contributions to endometrial progression and pathology, providing new leads for the potential causes of endometrial-factor infertility.

Association of blood cadmium and lead levels with self-reported reproductive lifespan and pregnancy loss: The national health and nutrition examination survey 1999-2018.

Cadmium and lead are known to interfere with the endocrine function. Thus, hormonally regulated processes such as menarche, menopause and pregnancy are likely influenced by chronic exposure to these metals. In US post-menopausal women, who already completed their reproductive lifespan, we evaluated the association between blood cadmium and lead levels with self-reported reproductive lifespan and personal history of pregnancy loss. We selected 5317 post-menopausal women participating in the National Health and Nutrition Examination Survey (NHANES), 1999-2018. Blood cadmium and lead levels were measured by inductively coupled plasma mass spectrometry. Reproductive lifespan was defined as the number of years between self-reported age at menarche and menopause. Personal history of pregnancy loss was defined as number of self-reported pregnancy losses out of the self-reported number of pregnancies. The fully adjusted mean difference in reproductive lifespan (95% confidence interval [CI]) comparing the 80th to the 20th percentiles of blood cadmium and lead distributions was, respectively, 0.50 (0.10, 0.91) and 0.72 (0.41, 1.03) years. Ever smoker showed stronger association of blood lead with reproductive lifespan. For self-reported pregnancy loss, the corresponding fully adjusted relative prevalence (95% CI) was 1.10 (0.93, 1.31) for cadmium and 1.10 (1.00, 1.21) for lead, and remained similar after additional adjustment for reproductive lifespan. In never smokers, the relative prevalence was 1.07 (1.04, 1.11) and 1.16 (1.05, 1.28) for blood cadmium and lead, respectively. These findings suggest that blood cadmium and lead exposures increase reproductive lifespan and prevalence of pregnancy loss in the general population. Additional studies are needed to improve the understanding of mechanisms and prevention potential of metals-related pregnancy outcomes.

Diseño y validez de contenido del cuestionario continuidad del cuidado entre niveles asistenciales / Design and content validity of the Continuity of Care Across Levels of Care Questionnaire

Introducción: La falta de continuidad del cuidado puede ocasionar omisiones o duplicaciones en las acciones dirigidas al cuidado de usuarios con Enfermedades Crónicas No Transmisibles (ECNT), generando un posible deterioro de su salud. Particularmente, en México y Colombia no existe un instrumento que evalúe la continuidad del cuidado que incluya sus tres elementos esenciales. Objetivos: Diseñar un instrumento que evalúe la continuidad del cuidado entre niveles asistenciales en usuarios con ECNT en México y Colombia; y validar el contenido del cuestionario por medio de un juicio de expertos en versiones adaptadas al contexto mexicano y colombiano. Materiales y Métodos: Se diseñó el cuestionario Continuidad del Cuidado entre Niveles Asistenciales. Se realizó el proceso de validación de contenido por expertos usando el método Delphi. Se seleccionaron 16 jueces expertos (8 por país). Los ítems del cuestionario fueron evaluados bajo cuatro categorías: suficiencia, claridad, coherencia y relevancia. Se realizaron dos rondas de evaluación para determinar el grado de concordancia entre jueces. Resultados: El cuestionario obtuvo un Coeficiente de Validez de Contenido General "Excelente" para ambos países (0,97). La versión final quedó conformada por 85 ítems divididos en tres secciones. Discusión: Este instrumento, a diferencia de otros, evalúa desde la experiencia de los usuarios con ECNT la continuidad del cuidado de forma multidisciplinaria en los tres niveles de atención. Conclusión: El cuestionario alcanzó una validez de contenido esperada usando el método Delphi, para evaluar la continuidad del cuidado entre niveles asistenciales en usuarios con ECNT según el contexto mexicano y colombiano.

Introduction: In the absence of continuity of care, actions aimed at the care of users with chronic non-communicable diseases (NCDs) may be omitted or duplicated, which can potentially worsen users' health. In Mexico and Colombia, in particular, there is no instrument for assessing continuity of care that includes its three essential elements. Objective: To develop an instrument to assess care continuity across levels of care for users with NCDs in Mexico and Colombia and validate the content of the questionnaire through expert judgment of versions adapted to the Mexican and Colombian contexts. Materials and Methods: The Continuity of Care across Levels of Care Questionnaire was designed. The content validation process was carried out by experts using the Delphi technique. Sixteen experts were selected (8 per country). Questionnaire items were assessed in four categories: sufficiency, clarity, coherence, and relevance. Two assessment rounds were conducted to determine the level of experts' agreement. Results: The questionnaire obtained an 'Excellent' overall Content Validity Coefficient in both countries (0.97). Discussion: This instrument, unlike others, assesses the continuity of care in a multidisciplinary manner across the three levels of care from the experience of users with NCDs. Conclusion: The questionnaire achieved the expected content validity using the Delphi technique to assess care continuity across levels of care for users with NCDs, according to the Mexican and Colombian contexts.

Introdução: A falta de continuidade do cuidado pode ocasionar omissões ou duplicidades nas ações voltadas para o atendimento aos usuários com Doenças Crônicas Não Transmissíveis (DCNT), gerando um possível agravamento de sua saúde. Particularmente, no México e na Colômbia não existe um instrumento que avalie a continuidade do cuidado que inclua seus três elementos essenciais. Objetivo: Desenhar um instrumento que avalie a continuidade do cuidado entre os níveis de atenção em usuários com DCNT no México e na Colômbia; e validar o conteúdo do questionário por meio de julgamento de especialistas em versões adaptadas ao contexto mexicano e colombiano. Materiais e Métodos: Foi elaborado o questionário de Continuidade de Cuidados entre os Níveis de Cuidados. O processo de validação de conteúdo foi realizado por especialistas por meio do método Delphi. Foram selecionados 16 juízes especialistas (8 por país). Os itens do questionário foram avaliados em quatro categorias: suficiência, clareza, coerência e relevância. Duas rodadas de avaliação foram realizadas para determinar o grau de concordância entre os juízes. Resultados: O questionário obteve um Coeficiente de Validade de Conteúdo Geral "Excelente" para ambos os países (0,97). A versão final foi composta por 85 itens divididos em três seções. Discussão: Este instrumento, diferente de outros, avalia a partir da experiência dos usuários com DCNT a continuidade do cuidado de forma multidisciplinar nos três níveis de atenção. Conclusão: O questionário atingiu a validade de conteúdo esperada usando o método Delphi, para avaliar a continuidade do cuidado entre níveis de atenção em usuários com DCNT de acordo com o contexto mexicano e colombiano.

Inhibition of Human Coronaviruses by Combinations of Host-Targeted and Direct-Acting Antivirals.

Antiviral compounds targeting cellular metabolism are part of the therapeutic arsenal to control the spread of virus infection, either as sole treatment or in combination with direct-acting antivirals (DAA) or vaccines. Here, we describe the effect of two of them, lauryl gallate (LG) and valproic acid (VPA) both exhibiting a wide antiviral spectrum, against infection by coronaviruses such as HCoV-229E, HCoV-OC43, and SARS-CoV-2. A consistent 2 to 4-log-decrease in virus yields was observed in the presence of each antiviral, with an average IC50 value of 1.6 µM for LG and 7.2 mM for VPA. Similar levels of inhibition were observed when adding the drug 1 h before adsorption, at the time of infection or 2 h after infection, supporting a postvirus entry mechanism of action. The specificity of the antiviral effect of LG against SARS-CoV-2, relative to other related compounds such as gallic acid (G) and epicatechin gallate (ECG), predicted to be better inhibitors according to in silico studies, was also demonstrated. The combined addition of LG, VPA, and remdesivir (RDV), a DAA with a proven effect against human coronaviruses, resulted in a robust synergistic effect between LG and VPA, and to a lesser extent between the other drug combinations. These findings reinforce the interest of these wide antiviral spectrum host-targeted compounds as a first line of defense against viral diseases or as a vaccine complement to minimize the gap in antibody-mediated protection evoked by vaccines, either in the case of SARS-CoV-2 or for other possible emerging viruses.

Deregulation of ζ-carotene desaturase in Arabidopsis and tomato exposes a unique carotenoid-derived redundant regulation of floral meristem identity and function.

A level of redundancy and interplay among the transcriptional regulators of floral development safeguards a plant's reproductive success and ensures crop production. In the present study, an additional layer of complexity in the regulation of floral meristem (FM) identity and flower development is elucidated linking carotenoid biosynthesis and metabolism to the regulation of determinate flowering. The accumulation and subsequent cleavage of a diverse array of ζ-carotenes in the chloroplast biogenesis 5 (clb5) mutant of Arabidopsis results in the reprogramming of meristematic gene regulatory networks establishing FM identity mirroring that of the FM identity master regulator, APETALA1 (AP1). The immediate transition to floral development in clb5 requires long photoperiods in a GIGANTEA-independent manner, whereas AP1 is essential for the floral organ development of clb5. The elucidation of this link between carotenoid metabolism and floral development translates to tomato exposing a regulation of FM identity redundant to and initiated by AP1 and proposed to be dependent on the E class floral initiation and organ identity regulator, SEPALLATA3 (SEP3).

Plastids: diving into their diversity, their functions, and their role in plant development.

Plastids are a group of essential, heterogenous semi-autonomous organelles characteristic of plants that perform photosynthesis and a diversity of metabolic pathways that impact growth and development. Plastids are remarkably dynamic and can interconvert in response to specific developmental and environmental cues, functioning as a central metabolic hub in plant cells. By far the best studied plastid is the chloroplast, but in recent years the combination of modern techniques and genetic analyses has expanded our current understanding of plastid morphological and functional diversity in both model and non-model plants. These studies have provided evidence of an unexpected diversity of plastid subtypes with specific characteristics. In this review, we describe recent findings that provide insights into the characteristics of these specialized plastids and their functions. We concentrate on the emerging evidence that supports the model that signals derived from particular plastid types play pivotal roles in plant development, environmental, and defense responses. Furthermore, we provide examples of how new technologies are illuminating the functions of these specialized plastids and the overall complexity of their differentiation processes. Finally, we discuss future research directions such as the use of ectopic plastid differentiation as a valuable tool to characterize factors involved in plastid differentiation. Collectively, we highlight important advances in the field that can also impact future agricultural and biotechnological improvement in plants.

Percepción de los estudiantes sobre el consumo de drogas psicoactivas en su universidad / Perception of Students Regarding Psychoactive Drug Use at Their University / Percepção de estudantes sobre o consumo de drogas psicoativas na universidade

entre los jóvenes universitarios se ha observado una tolerancia creciente hacia el consumo de drogas, debido ­entre otros factores­ a una reducción en la percepción de riesgo, que puede con-tribuir a la normalización del consumo. El objetivo fue analizar la percepción de los estudiantes de unauniversidad pública mexicana sobre la situación y la gravedad del consumo de drogas en su institución mediante diversos indicadores. Materiales y métodos: se analizó la base de datos derivada de la aplica-ción del Cuestionario sobre Consumo de Drogas en Estudiantes Universitarios (codeu-3), en el que partici-paron 19 815 estudiantes; se realizaron comparaciones según sexo y área académica utilizando pruebas de chi-cuadrado y el análisis post hoc de Bonferroni. Resultados: las mujeres indicaron tener una menor percepción de riesgo que los hombres sobre las variables analizadas (percepción de los participantes sobre el consumo de drogas en su facultad, venta y gravedad del consumo de drogas, según sexo y área académica y conductas de docentes y estudiantes que facilitan el consumo de alcohol y la postura de las autoridades universitarias ante estudiantes consumidores, según sexo y área académica). Conclusión: los resultados muestran diferencias en la percepción de hombres y mujeres, así como las diferencias asociadas con la adscripción de los estudiantes en las distintas áreas académicas, por lo que se sugiere profundizar el estudio de estos aspectos.

There is a growing tolerance toward drug use among university students due to a reduction in the perception of risk, which is considered as those judgments or intuitions held regarding the danger of a situation that can contribute to the normalization of consumption. This study aimed to analyze the perception of the students at a Mexican public university toward the situation and seriousness of drug use in the university using various indicators. Materials and methods: The database derived from the application of the Questionnaire on Drug Use in University Students (codeu-2 by its name in Spanish), in which 19,815 students from a Mexican public university participated, was analyzed, and comparisons were made according to gender and academic area using chi-squared tests and Bonferroni's post hocanalysis. Results: Women were reported to have a lower perception of risk than men on the variables analyzed (participants' perception of drug use in their faculty, sale and severity of drug use, according to gender and academic area, and behaviors of teachers and students that facilitate alcohol use and the position of university authorities towards student consumers, according to gender and academic area). Conclusion: The results indicated the importance of studying in greater depth the differences in the perception of men and women, as well as their affiliation in the different academic areas.

tem-se observado entre os jovens universitários uma crescente tolerância ao uso de dro-gas, devido à redução da percepção de risco, que é considerada como aqueles julgamentos ou intuições que se fazem sobre o perigo de uma situação, o que pode contribuir para a normalização do consumo. O objetivo do estudo foi analisar a percepção dos estudantes de uma universidade pública mexicana sobre a situação e a gravidade do uso de drogas em sua instituição por meio de vários indicadores. Materiais e métodos: analisou-se a base de dados derivada da aplicação do Questionário de Uso de Drogas em Estudantes Universitários (codeu-2), do qual participaram 19,815 estudantes; as comparações foram feitas de acordo com o gênero e a área acadêmica por meio de testes de qui-quadrado e análise post hoc de Bonferroni. Resultados: as mulheres relataram menor percepção de risco do que os homens nas variáveis analisadas (percepção dos participantes sobre o uso de drogas na faculdade, venda e gravidade do uso de drogas, por gênero e área acadêmica, e comportamentos de professores e alunos que facilitam o consumo de álcool e a atitude das autoridades universitárias perante estudantes consumidores, de acordo com o gênero e área acadêmica). Conclusão: os resultados mostram diferenças na percepção de homens e mulheres, bem como as diferenças associadas à inserção dos alunos nas diferentes áreas acadêmicas, para o que se sugere aprofundar o estudo desses aspectos.

Association of Co-Living and Age on the Type of Sports Practiced by Older People.

Introdution: The environment and the type of co-living of older people are crucial to understanding how the nature of their context influences a healthy lifestyle. However, no studies have investigated to what extent their type of co-living may be associated with the sports and the profile practice according to their age. Objective: This study aimed to assess the association between the types of co-living and the age of the physically active elderly and the sports they practice. Hypothesis: It is thought that the age, situation, and satisfaction with the way of living in physically active older people differ according to the type of sport they practice. Methods: Cross-sectional study. We included 358 individuals aged between 61 and 93 years old (M = 69.66, SD = 4.74). Type of co-living was classified as living alone or living with others. The sports activities evaluated were: gym, dance, water activities, and yoga/pilates. Differences in the type of co-living and sports practiced were evaluated by ANOVA or Chi2. Results: Among the elderly who practice gymnastics, most of them live alone and in a tight core (p < 0.001) (Phi = 0.244). Furthermore, those who practice aquatic activities are more frequently the youngest (p < 0.001) (Phi = 0.198). Conclusion: Older people who do gymnastics have smaller living groups, those under 69 opt for gymnastics and aquatic activities, while those aged 70 and over prefer dance, yoga, and pilates.

Parasitosis y antígenos TF, Tn y sTn / Parasitosis and TF, Tn and sTn antigens

Resumen Las investigaciones realizadas establecen una relación entre los epítopos T, Tn y sTn y las enfermedades parasitarias. Estos epítopos se expresan en un alto porcentaje de tumores epiteliales e inicialmente fueron relacionados con el síndrome T, caracterizado por trombocitopenia, leucopenia y anemia hemolítica. Se ha identificado la expresión de Tn en varios carcinomas, aunque los eventos asociados a su exposición en éstos parecen ser diferentes de los observados en el síndrome Tn. Diversos estudios comunicaron que estructuras asociadas a tumores, tales como los antígenos Tn y sialil-Tn, se expresan en algunos protozoarios y helmintos, y plantearon numerosos interrogantes a nivel de la interacción parásito-hospedador, de la glicobiología parasitaria y de las eventuales relaciones entre la biología de algunos parásitos y las células cancerígenas. Los hematíes son poliaglutinables cuando son aglutinados por casi todas las muestras de suero humano normal. Algunas formas de poliaglutinidad se deben a la exposición del determinante antigénico TF, mediante la eliminación del ácido N-acetilneuramínico, por la acción de neuraminidasas bacterianas o virales, aunque en los últimos años se ha comunicado el desenmascaramiento de este antígeno críptico eritrocitario por Ascaris lumbricoides y Trichinella spiralis. Debido a la importancia clínica de la activación T, se destaca la necesidad de estudiar la exposición del antígeno críptico TF en todos los parásitos cuyo hábitat sea la sangre, o bien en aquellos cuyos ciclos de vida comprendan una migración por el torrente circulatorio, pues su desenmascaramiento puede ocasionar autoaglutinación y/o hemólisis.

Abstract The investigations carried out establish a relationship between the T, Tn and sTn epitopes and the parasitic diseases. These epitopes are expressed in a high percentage of epithelial tumors, and they were initially related to the T syndrome, characterised by thrombocytopenia, leukopenia, and hemolytic anemia. The expression of Tn has been identified in several carcinomas, although the events associated with its exposure appears to be different from those observed in the Tn syndrome. Various studies report that tumor-associated structures such as Tn and sialyl-Tn antigens are expressed in some protozoa and helminths, raising numerous questions at the level of parasite-host interaction, parasitic glycobiology and eventual relationships between the biology of some parasites and cancer cells. Red cells are polyaglutinate when agglutinated by almost all normal human serum samples. Some forms of polyaglutinity are due to the exposure of the antigenic determinant TF, through the elimination of N-acetylneuraminic acid, by the action of bacterial or viral neuraminidases, although the unmasking of this erythrocyte cryptic antigen by Ascaris lumbricoides and Trichinella spiralis has been reported in recent years. Due to the clinical importance of T activation, the need to study the exposure of the cryptic TF antigen in all parasites whose habitats are blood or whose life cycles include migration through the circulatory stream is highlighted, since its unmasking can cause autoagglutination and/or hemolysis.

Resumo As investigações realizadas estabelecem uma relação entre os epítopos T, Tn e sTn e doenças parasitárias. Esses epítopos são expressos em alta porcentagem de tumores epiteliais e foram inicialmente relacionados à síndrome T, caracterizada por trombocitopenia, leucopenia e anemia hemolítica. A expressão de Tn foi identificada em vários carcinomas, embora os eventos associados à sua exposição neles pareçam ser diferentes dos observados na síndrome Tn. Vários estudos relatam que estruturas associadas a tumores, tais como antígenos Tn e sialil-Tn, são expressos em alguns protozoários e helmintos, levantando inúmeras questões no nível da interação parasita-hospedeiro, glicobiologia parasitária e eventuais relações entre a biologia de alguns parasitas e células cancerígenas. Os glóbulos vermelhos são poliaglutináveis quando são aglutinados por quase todas as amostras de soro humano normal. Algumas formas de poliaglutinidade são devidas à exposição do determinante antigênico TF, pela eliminação do ácido N-acetilneuramínico, pela ação de neuraminidases bacterianas ou virais, embora nos últimos anos tenha sido relatado o desmascaramento desse antígeno eritrocitário críptico por Ascaris lumbricoides e Trichinella spiralis. Devido à importância clínica da ativação T, destaca-se a necessidade de estudar a exposição do antígeno críptico TF em todos os parasitas cujo habitat seja o sangue ou naqueles cujos ciclos de vida incluam migração pela corrente circulatória, uma vez que seu desmascaramento pode causar autoaglutinação e/ou hemólise.

Variables predictoras de la calidad de vida durante la pandemia de Covid-19 en adultos latinoamericanos

Objetivo: Evaluar las variables predictoras de la calidad de vida en población adulta latinoamericana en situación de pandemia durante la cuarentena por COVID-19. Métodos: Estudio descriptivo, trasversal. La muestra estuvo compuesta por 3101 habitantes adultos de Chile, Colombia, México y Perú. Los factores que aumentaron la probabilidad de tener una baja calidad de vida se identificaron mediante un análisis de regresión logística. Estos análisis fueron ajustados por estado nutricional, edad y procedencia geográfica. Resultados: Ser de género femenino (OR=1.73; p=0.001), físicamente inactivo/a (OR=1.85; p=0.001), consumir tabaco (OR=1.29; p=0,026), alcohol (OR=1.31; p=0,002) y comida chatarra (OR=2.04; p=0,001) aumentaron la probabilidad de tener una disminución en la dimensión salud general de la calidad de vida durante una cuarentena por Covid-19. Conclusiones: Los hallazgos en este estudio confirman la necesidad de promover hábitos y estilos de vida saludables en la población durante las cuarentenas en una pandemia como, por ejemplo, una dieta sana, practicar actividad física y evitar estar mucho tiempo sentado.

Objective: To evaluate the predictive variables of the quality of life in the Latin American adult population in a pandemic situation during the Covid-19 quarantine. Methods: Descriptive, cross-sectional study. The population was composed of 3,101 adult inhabitants of Chile, Colombia, Mexico and Peru. Factors that increased the likelihood of poor quality of life were identified using logistic regression analysis. These analyzes were adjusted for nutritional status, age, and geographic origin. Results: Being female (OR=1.73; p=0.001), physically inactive (OR=1.85; p=0.001), consuming tobacco (OR=1.29; p=0.026), alcohol (OR=1.31; p=0.002) and junk food (OR=2.04; p=0.001) increased the probability of having a decrease in the general health dimension of quality of life during a Covid-19 quarantine. Conclusions: The findings in this study confirm the need to promote healthy habits and lifestyles in the population during quarantines in a pandemic, such as a healthy diet, practicing physical activity and avoiding prolonged sitting.

Endometrial gene expression differences in women with coronavirus disease 2019.

OBJECTIVE: To study the potential effect of coronavirus disease (COVID-19) on the endometrial transcriptome of affected, symptomatic women for the detection of altered gene expression. DESIGN: Pilot study of the endometrial transcriptomes of women manifesting COVID-19 compared with those of women without COVID-19 undergoing hysteroscopic procedures for benign gynecologic disorders using RNA sequencing. SETTING: Hospital and university laboratories. PATIENT(S): Women with (n = 14) and without a COVID-19 (n = 10) diagnosis based on a nasopharyngeal swab analysis using quantitative reverse-transcription polymerase chain reaction. The endometrium of the patients with COVID-19 had previously been tested for severe acute respiratory syndrome coronavirus 2 infection, revealing the absence of the virus in this tissue. INTERVENTION(S): Endometrial biopsy sample collection. MAIN OUTCOMES MEASURE(S): Endometrial gene expression and functional analysis of symptomatic patients with COVID-19 vs. individuals without the infection. RESULT(S): The systemic disease COVID-19 altered endometrial gene expression in 75% of the women, with the patients exhibiting a preponderance of 163 up-regulated (e.g., UTS2, IFI6, IFIH1, and BNIP3) and 72 down-regulated genes (e.g., CPZ, CDH3, and IRF4) (false discovery rate<0.05). A total of 161 dysregulated functions (36 up-regulated and 125 down-regulated) were typically enriched in the endometria of the patients with COVID-19, including up-regulation in pathways involved in the development of immune responses to viruses and cytokine inflammation, reflecting elicitation of a COVID-19 response pathway. CONCLUSION(S): Coronavirus disease 2019 affects endometrial gene expression despite the absence of severe acute respiratory syndrome coronavirus 2 RNA in endometrial tissues.

Consumo de drogas legales e ilegales y síntomas de depresión en adultos mayores usuarios de Facebook durante la pandemia por SARS-CoV-2 en México / Consumption of legal and illegal drugs and depressive symptoms in older adults during the SARS-CoV-2 pandemic in Mexico

Antecedentes: Los riesgos para la salud que enfrentan los adultos mayores son diversos; sin embargo, poco se ha explorado acerca del uso y abuso de sustancias psicoactivas en esta población. La reclusión impuesta por la situación que prevalece debido al SARS-CoV-2 ha incrementado los sentimientos de soledad, aislamiento y tristeza asociados a esta edad, que los convierte en un factor de riesgo para el consumo de drogas. Objetivo: Analizar el consumo de drogas legales e ilegales en el personas mayores de 60 años usuarios de Facebook®, y su relación con síntomas de depresión durante la pandemia por SARS-CoV-2.Material y métodosEstudio realizado a 380 personas mayores, usuarios de Facebook®, que respondieron un cuestionario publicado en línea, que indagó sobre: datos sociodemográficos, frecuencia y cantidad de consumo de drogas legales e ilegales y sintomatología depresiva. Resultados: El 50,26% fueron mujeres; la edad promedio fue de 6,79 años (DS=5,81); el 31,05% consumieron alcohol en los últimos 30 días, el 22,63% tabaco, tranquilizantes sin prescripción médica el 16,05% y mariguana el 7,89%. El consumo de otras drogas ilegales no superaron al 2,6% de la población. Al comparar entre consumidores y no consumidores, resultó que el consumo en los últimos 30 días fue ligeramente mayor en mujeres y en solteros, y no se observaron diferencias en función del nivel de escolaridad. Los síntomas de depresión leve y grave se encontró asociada con todas las drogas a excepción de tabaco y opiáceos. Discusión y conclusiones: Los resultados obtenidos demuestran la necesidad de visibilizar el consumo de drogas entre los adultos mayores, y de desarrollar estrategias que disminuyan las alteraciones anímicas que pueden estar experimentando, como el miedo, la angustia y la depresión. (AU)

Background: The health risks faced by older adults are diverse; however, little has been explored about the use and abuse of psychoactive substances in this population. The seclusion imposed by the situation that prevails due to SARS-CoV-2 has increased the feelings of loneliness, isolation and sadness associated with this age, which makes them a risk factor for drug use. Objective: To analyze the consumption of legal and illegal drugs in people over 60 years of age who are Facebook users and its relationship with symptoms of depression during the SARS-CoV-2 pandemic. Material and methods: Study carried out on 380 elderly people, Facebook users, who answered a questionnaire published online, which inquired about: sociodemographic data, frequency and amount of legal and illegal drug use, and depressive symptomatology. Results: 50.26% were women; the average age was 66.79 years (SD=5.81); 31.05% consumed alcohol in the last 30 days, 22.63% tobacco, tranquilizers without medical prescription 16.05% and marijuana 7.89%. The consumption of other illegal drugs did not exceed 2.6% of the population. When comparing between users and non-users, it turned out that consumption in the last 30 days was slightly higher in women, in single people and no differences were observed depending on the level of schooling. Mild and severe depressive symptoms were found to be associated with all drugs except tobacco and opiates. Discussion and conclusions: The results obtained demonstrate the need to make drug use visible among older adults and to develop strategies that reduce the mood disorders they may be experiencing, such as fear, anguish and depression. When comparing between users and non-users, it turned out that consumption in the last 30 days was slightly higher in women, in single people and no differences were observed depending on the level of schooling. (AU)

Accurate Computational Prediction of Core-Electron Binding Energies in Carbon-Based Materials: A Machine-Learning Model Combining Density-Functional Theory and GW.

We present a quantitatively accurate machine-learning (ML) model for the computational prediction of core-electron binding energies, from which X-ray photoelectron spectroscopy (XPS) spectra can be readily obtained. Our model combines density functional theory (DFT) with GW and uses kernel ridge regression for the ML predictions. We apply the new approach to disordered materials and small molecules containing carbon, hydrogen, and oxygen and obtain qualitative and quantitative agreement with experiment, resolving spectral features within 0.1 eV of reference experimental spectra. The method only requires the user to provide a structural model for the material under study to obtain an XPS prediction within seconds. Our new tool is freely available online through the XPS Prediction Server.

[Consumption of legal and illegal drugs and depressive symptoms in older adults during the SARS-CoV-2 pandemic in Mexico]. / Consumo de drogas legales e ilegales y síntomas de depresión en adultos mayores usuarios de Facebook durante la pandemia por SARS-CoV-2 en México.

BACKGROUND: The health risks faced by older adults are diverse; however, little has been explored about the use and abuse of psychoactive substances in this population. The seclusion imposed by the situation that prevails due to SARS-CoV-2 has increased the feelings of loneliness, isolation and sadness associated with this age, which makes them a risk factor for drug use. OBJECTIVE: To analyze the consumption of legal and illegal drugs in people over 60 years of age who are Facebook users and its relationship with symptoms of depression during the SARS-CoV-2 pandemic. MATERIAL AND METHODS: Study carried out on 380 elderly people, Facebook users, who answered a questionnaire published online, which inquired about: sociodemographic data, frequency and amount of legal and illegal drug use, and depressive symptomatology. RESULTS: 50.26% were women; the average age was 66.79 years (SD=5.81); 31.05% consumed alcohol in the last 30 days, 22.63% tobacco, tranquilizers without medical prescription 16.05% and marijuana 7.89%. The consumption of other illegal drugs did not exceed 2.6% of the population. When comparing between users and non-users, it turned out that consumption in the last 30 days was slightly higher in women, in single people and no differences were observed depending on the level of schooling. Mild and severe depressive symptoms were found to be associated with all drugs except tobacco and opiates. DISCUSSION AND CONCLUSIONS: The results obtained demonstrate the need to make drug use visible among older adults and to develop strategies that reduce the mood disorders they may be experiencing, such as fear, anguish and depression. When comparing between users and non-users, it turned out that consumption in the last 30 days was slightly higher in women, in single people and no differences were observed depending on the level of schooling.

The role of carotenoids as a source of retrograde signals: impact on plant development and stress responses.

Communication from plastids to the nucleus via retrograde signal cascades is essential to modulate nuclear gene expression, impacting plant development and environmental responses. Recently, a new class of plastid retrograde signals has emerged, consisting of acyclic and cyclic carotenoids and/or their degradation products, apocarotenoids. Although the biochemical identity of many of the apocarotenoid signals is still under current investigation, the examples described herein demonstrate the central roles that these carotenoid-derived signals play in ensuring plant development and survival. We present recent advances in the discovery of apocarotenoid signals and their role in various plant developmental transitions and environmental stress responses. Moreover, we highlight the emerging data exposing the highly complex signal transduction pathways underlying plastid to nucleus apocarotenoid retrograde signaling cascades. Altogether, this review summarizes the central role of the carotenoid pathway as a major source of retrograde signals in plants.

Female orgasmic dysfunction and severe climacteric symptomatology in women aged 40 to 59 years: an independent association from an analysis of a multicenter Latin American study.

OBJECTIVE: To evaluate the association between the severity of climacteric symptoms (CS) and orgasmic dysfunction (OD), controlled by demographic, clinical, and partner variables. METHODS: We carried out a secondary analysis of a multicenter Latin American cross-sectional study that surveyed sexually active women 40 to 59 years old. We assessed CS (global, somatic, psychological, or urogenital domains) and OD. Also, we explored clinical variables and partner sexual conditions. We performed logistic regression models with nonparametric bootstrap resampling to estimate crude and adjusted odds ratios (aOR) with 95% confidence intervals (CI). RESULTS: We included data of 5,391 women in the analysis. Regarding CS, 24.8%, 10.8%, 28.4%, and 32.9% had respectively severe symptoms according to total, somatic, psychological, and urogenital domain scores of the Menopause Rating Scale. OD was found in 25.4% of women. The adjusted model (including menopausal status and partner sexual dysfunction) showed that severe CS increased the odds of OD (aOR = 2.77; 95% CI: 2.41-3.19 [total Menopause Rating Scale score]; aOR = 1.65; 95% CI: 1.37-2.00 [somatic domain]; aOR = 2.02; 95% CI: 1.76-2.32 [psychological domain] and aOR = 3.89; 95% CI: 3.40-4.45 [urogenital]). CONCLUSIONS: Severe CS were associated with OD independently of demographic, clinical, and partner variables. Severe urogenital symptoms had the strongest association.