Development of a sensitive electrochemical method to determine amitraz based on perylene tetracarboxylic acid/mesoporous carbon/Nafion@SPCEs.

A cutting-edge electrochemical method is presented for precise quantification of amitraz (AMZ), a commonly used acaricide in veterinary medicine and agriculture. Leveraging a lab-made screen-printed carbon electrode modified with a synergistic blend of perylene tetracarboxylic acid (PTCA), mesoporous carbon (MC), and Nafion, the sensor's sensitivity was significantly improved. Fine-tuning of PTCA, MC, and Nafion ratios, alongside optimization of the pH of the supporting electrolyte and accumulation time, resulted in remarkable sensitivity enhancements. The sensor exhibited a linear response within the concentration range 0.01 to 0.70 µg mL-1, boasting an exceptionally low limit of detection of 0.002 µg mL-1 and a limit of quantification of 0.10 µg mL-1, surpassing maximum residue levels permitted in honey, tomato, and longan samples. Validation with real samples demonstrated high recoveries ranging from 80.8 to 104.8%, with a relative standard deviation below 10%, affirming the method's robustness and precision. The modified PTCA/MC/Nafion@SPCE-based electrochemical sensor not only offers superior sensitivity but also simplicity and cost-effectiveness, making it a pivotal tool for accurate AMZ detection in food samples. Furthermore, beyond the scope of this study, the sensor presents promising prospects for wider application across various electrochemical analytical fields, thereby significantly contributing to food safety and advancing agricultural practices.

A novel immunosensor based on cobalt oxide nanocomposite modified single walled carbon nanohorns for the selective detection of aflatoxin B1.

We developed a novel, sensitive, and selective platform for the specific determination of aflatoxin B1 (AFB1). Single-walled carbon nanohorns decorated by a cobalt oxide composite and gold nanoparticles were created to provide facile electron transfer and improve the sensor's sensitivity. In addition, we attributed the selectivity of the proposed sensor to the specific binding property of the anti-aflatoxin B1 antibody. We clarified the specific interaction of the proposed immunosensor to AFB1 using homology modeling combined with molecular docking. In the presence of AFB1, the current signal of the modified electrode reduced; this involved specific antibody-antigen binding, including hydrophobic hydrogen bonding and pi-pi stack interactions. The new AFB1 sensor platform showed two linearity ranges of 0.01-1 ng mL-1 and 1-100 ng mL-1, with the limit of detection at 0.0019 ng mL-1. We investigated the proposed immunosensor in real samples, including peanuts, certified reference material of a peanut sample (labeled 206 µg kg-1 AFB1), corn, and chicken feed. The sensor's accuracy was 86.1-104.4% recovery, which agrees with the reference HPLC technique using paired t-test analysis. The present work shows excellent performance for AFB1 detection and could be applied for food quality control or modified to detect other mycotoxins.

A novel and portable electrochemical sensor for 5-hydroxymethylfurfural detection using silver microdendrite electrodeposited paper-based electrode.

A portable paper-based electrochemical sensor has been developed to determine 5-hydroxymethylfurfural (5-HMF). A screen-printed carbon electrode (SPCE) was facilely fabricated for the first time on poster paper which showed a very satisfactory electrochemical response. The analytical performance of the electrode was enhanced by electrochemical deposition of silver microdendrites (AgMDs). The cathodic peak of 5-HMF occurred at approximately -1.48 V, lower than that obtained from the bare poster-SPCE. Moreover, the modified electrode showed a higher current response than the bare electrode, revealing that the AgMDs not only exhibited highlighted electrocatalytic features but also improved the electrical conductivity and increased the electrode surface area. Afterward, some influencing conditions were optimized, including scan rate and the number of scan cycles for AgMD deposition, pH, temperature, and square wave voltammetric parameters. Under the optimal conditions, the analytical characteristics of the proposed sensor were evaluated. The cathodic peak current increased linearly according to 5-HMF concentration over the range of 3-100 ppm, and the detection limit was 1.0 ppm. This low-cost, disposable electrochemical sensor provided environmentally friendly, simple and rapid detection, acceptable precision, good stability, and high selectivity. Additionally, this method can be applied to quantify 5-HMF in honey samples with satisfying accuracy.

A facile colorimetric aptasensor for low-cost chlorpyrifos detection utilizing gold nanoparticle aggregation induced by polyethyleneimine.

A colorimetric aptasensor for chlorpyrifos detection utilizing the localized surface plasmon resonance (LSPR) of gold nanoparticle (AuNP) aggregates coupling with a specific aptamer and cationic polyethyleneimine (PEI) has been developed. The measurement principle is based on a remarkable characteristic of AuNPs that can change their colors under the aggregation and dispersion conditions, which enables a sensitive colorimetric detection. In the absence of chlorpyrifos, negatively charged phosphate backbones of the aptamer potentially interact with the cationic PEI, resulting in the red color appearance of the dispersed AuNPs, whereas, in the presence of chlorpyrifos, the aptamer binds explicitly to chlorpyrifos, consequently releasing cationic PEI. Uninteracted PEI induces AuNP aggregation, causing a color change from red to blue that can be observed through the naked eye. Under the optimized conditions, 6 nM PEI, 10 nM aptamer, and a pH buffer of 7.5, the colorimetric aptasensor gives a linear response in the range of 20-300 ng mL-1 with a low detection limit of 7.4 ng mL-1. The developed method has been successfully applied to complex sample analysis. The accuracy and precision of chlorpyrifos quantification in spiked samples, including tap water, pomelo, and longan samples, are in the acceptable criteria of method validation, indicating that the developed aptasensor can be utilized as an alternative analytical tool for chlorpyrifos determination in complex samples. This aptasensor provides advantages such as a simple procedure, low cost, short analysis time, and involving uncomplicated instruments. Moreover, it offers high sensitivity, selectivity, and stability.