In Vitro Intestinal and Liver Models for Toxicity Testing.

The human body is exposed to hundreds of chemicals every day. Many of these toxicants have unknown effects on the body that can be deleterious. Furthermore, chemicals can have a synergistic effect, resulting in toxic responses of cocktails at relatively low individual exposure levels. The gastrointestinal (GI) tract and the liver are the first organs to be exposed to ingested pharmaceuticals and environmental chemicals. As a result, these organs often experience extensive damage from xenobiotics and their metabolites. In vitro models offer a promising method for testing toxic effects. Many advanced in vitro models have been developed for GI and liver toxicity. These models strive to recapitulate the in vivo organ architecture to more accurately model chemical toxicity. In this review, we discuss many of these advances, in addition to recent efforts to integrate the GI and the liver in vitro for a more holistic toxicity model.

The promise of organotypic hepatic and gastrointestinal models.

Advances in the design and assembly of in vitro organotypic liver and gastrointestinal (GI) models can accelerate our understanding of metabolism, nutrient absorption, and the effect of microbial flora. Such models can provide comprehensive information on how of environmental toxins, drugs, and pharmaceuticals interact with and within these organs. Information obtained from such models could elucidate the complicated cascades of signaling mechanisms that occur in vivo. Because experiments on large-scale animal models are expensive and resource intensive, the design of organotypic models has renewed significance. The challenges and approaches to designing liver and GI models are similar. Because these organs are in close proximity and interact continually, we have described recent design considerations to guide future tissue models.