RESUMO
A stereomicroscope system is adapted to make accurate, quantitative displacement, and strain field measurements with microscale spatial resolution and nanoscale displacement resolution on mouse carotid arteries. To perform accurate and reliable calibration for these systems, a two-step calibration process is proposed and demonstrated using a modification to recently published procedures. Experimental results demonstrate that the microscope system with three-dimensional digital image correlation (3D-DIC) successfully measures the full 3D displacement and surface strain fields at the microscale during pressure cycling of 0.40-mm-diameter mouse arteries, confirming that the technique can be used to quantify changes in local biomechanical response which may result from variations in extracellular matrix composition, with the goal of quantifying properties of the vessel.