[Histological study of urethral plate: a search of the best material for surgical treatment of hypospadias in children].

AIM: to study the microstructure of the urethral plate in patients with a proximal form of hypospadias to justify the use of tissues from the ventral penile surface when creating an artificial urethra. MATERIALS AND METHODS: A histological study of surgical specimens from 10 patients with proximal hypospadias was carried out. The urethral plate, a strip of tissue from the ectopic external urethral orifice located in the penoscrotal angle, to the coronary sulcus, was completely excised and processed by various techniques. A staining with hematoxylin and eosin, picrofuchsin according to Van Gieson, as well as immunohistochemical typing of CD 34, collagen (type I, III, and IV), fibronectin, and smooth myocytes using alpha-smooth muscle actin was performed. RESULTS: All elements which are present in a healthy urethra, such as epithelial cells, blood vessels, normal collagen fibers, smooth muscle cells and complexes of glands, were found in the microstructure of the urethral plate. CONCLUSION: The cytoarchitectonic features of the ventral penile tissues are similar to the microstructure of the healthy urethra, which provides a rationale to consider the urethral plate as a material with optimal bioresistant properties for urethroplasty.

[ROLE OF TGFß-INDUCTION AND GASTROINTESTINAL MYOFIBROBLASTS IN PATHOMORPHOGENESIS OF CHRONIC GASTRITIS IN PATIENTS WITH MARFAN SYNDROME AND MARFAN-LIKE STATES].

Aim: The aim was to explore the features of epithelial morphogenesis and reparation of gastric mucosa in chronic gastritis in patients with Marfan syndrome and Marfan -like states. Materials and methods: A histological evaluation and immunohistochemical analysis of the expression of antibodies against α-actin smooth muscle (α-SMA), collagen type III and transforming growth factor ß (TGFß) in the gastric mucosa in 50 patients with hereditary disorders of connective tissue between the ages of 17 to 41 years were performed. Results: In patients with Marfan syndrome and marfan-like states chronic gastritis was an unfavorable factor for the progression of the background atrophic and fibrotic processes in the gastric mucosa. It proved a key role disinhibited to TGFß activity-dependent activation of fibrogenesis of subepithelial myofibroblasts (α-SMA +) with the development of a special morphogenetic variants of early chronic atrophic multifocal gastritis at a young age.