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The Pearl River Estuary (PRE), one of the primary e-waste recycling centers in the world, has been suffering from the pollution of Liquid Crystal Monomers (LCMs), critical materials with persistent, bio-accumulative, and toxic substances used in electronic devices. It has been detected in seabed sediment with both high frequency and concentration near PRE - Hong Kong (HK) waters. In the same area, dredging operations with in-situ sediment have been frequently used in the last decades for coastal land reclamation projects. Dredging is known to cause a huge amount of sediment re-suspension into water columns, with potential damage to marine ecosystems and biodiversity. In this study, we proposed a new risk assessment strategy to estimate the secondary pollution due to the re-suspension sediment highly contaminated by LCMs. We formulate a robust and reliable probabilistic approach based on unsupervised machine learning and hydrodynamic and sediment transport numerical simulation. New risk indexes were also proposed to better quantify the impact of contaminated sediments. We applied the methodology to assess the potential impact of dredging operations in the PRE and Hong Kong waters on the local marine ecosystem. The results of the analysis showed how the potentially contaminated areas depended on the dredging locations.
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The outbreak of the COVID-19 pandemic led to a sharp increase in disposable surgical mask usage. Discarded masks can release microplastic and cause environmental pollution. Since masks have become a daily necessity for protection against virus infections, it is necessary to review the usage and disposal of masks during the pandemic for future management. In this study, we constructed a dynamic model by introducing related parameters to estimate daily mask usage in 214 countries from January 22, 2020 to July 31, 2022. And we validated the accuracy of our model by establishing a dataset based on published survey data. Our results show that the cumulative mask usage has reached 800 billion worldwide, and the microplastics released from discarded masks due to mismanagement account for 3.27% of global marine microplastic emissions in this period. Furthermore, we illustrated the response relationship between mask usage and the infection rates. We found a marginally significant negative correlation existing between the mean daily per capita mask usage and the rate of cumulative confirmed cases within the range of 25% to 50%. This indicates that if the rate reaches the specified threshold, the preventive effect of masks may become evident.
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COVID-19 , Máscaras , Modelos Teóricos , COVID-19/epidemiologia , COVID-19/prevenção & controle , Humanos , Pandemias , Microplásticos/análise , SARS-CoV-2RESUMO
The investigation of pharmaceuticals as emerging contaminants in marine biota has been insufficient. In this study, we examined the presence of 51 pharmaceuticals in edible oysters along the coasts of the East and South China Seas. Only nine pharmaceuticals were detected. The mean concentrations of all measured pharmaceuticals in oysters per site ranged from 0.804 to 15.1 ng g-1 of dry weight, with antihistamines being the most common. Brompheniramine and promethazine were identified in biota samples for the first time. Although no significant health risks to humans were identified through consumption of oysters, 100-1000 times higher health risks were observed for wildlife like water birds, seasnails, and starfishes. Specifically, sea snails that primarily feed on oysters were found to be at risk of exposure to ciprofloxacin, brompheniramine, and promethazine. These high risks could be attributed to the monotonous diet habits and relatively limited food sources of these organisms. Furthermore, taking chirality into consideration, chlorpheniramine in the oysters was enriched by the S-enantiomer, with a relative potency 1.1-1.3 times higher when chlorpheniramine was considered as a racemate. Overall, this study highlights the prevalence of antihistamines in seafood and underscores the importance of studying enantioselectivities of pharmaceuticals in health risk assessments.
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Monitoramento Ambiental , Ostreidae , Preparações Farmacêuticas , Poluentes Químicos da Água , Animais , Humanos , Bromofeniramina/análise , China , Clorfeniramina/análise , Antagonistas dos Receptores Histamínicos/análise , Oceanos e Mares , Ostreidae/química , Preparações Farmacêuticas/análise , Prometazina/análise , Poluentes Químicos da Água/análiseRESUMO
The persistence of extracellular antibiotic resistance genes (ARGs) in aquatic environments has attracted increasing attention due to their potential threat to public health and the environment. However, the fate of extracellular ARGs in receiving water remains largely unknown. This study investigated the influence of hematite nanoparticles, a widespread natural mineral, on the photodegradation of extracellular ARGs in river water. Results showed that under exposure to visible light, hematite nanoparticles, at environmental concentrations, resulted in a 3-5 orders of magnitude reduction in extracellular ARGs. This photodegradation of extracellular ARGs is shown to be facet-dependent; the (001) facet of hematite demonstrates a higher removal rate than that of the (100) facet, which is ascribed to its enhanced adsorption capability and higher hydroxyl radical (â¢OH) production. Density functional theory (DFT) calculations corroborate this finding, indicating elevated iron density, larger adsorption energy, and lower energy barrier of â¢OH formation on the (001) facet, providing more active sites and â¢OH generation for extracellular ARG interaction. Gel electrophoresis and atomic force microscopy analyses further confirm that the (001) facet causes more substantial damage to extracellular ARGs than the (100) facet. These findings pave the way for predicting the photodegradation efficiency of hematite nanoparticles with varied facets, thereby shedding light on the inherent self-purification capacity for extracellular ARGs in both natural and engineered aquatic environments.
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Antibacterianos , Águas Residuárias , Antibacterianos/farmacologia , Genes Bacterianos , Água , Resistência Microbiana a Medicamentos/genética , Nanopartículas Magnéticas de Óxido de FerroRESUMO
Amphibians are sensitive biomonitors of environmental pollutants but reports regarding per- and polyfluoroalkyl substances (PFAS), a class of synthetic organofluorine substances, are limited. In this study, samples of water and Chinese toads (Bufo gargarizans) were collected in Chaohu Lake, China. Tissue-specific bioaccumulation characteristics of 39 PFAS, including 19 perfluoroalkyl acids (PFAAs), 8 emerging PFAS, and 12 PFAA precursors, were investigated, and the levels of some biochemical indicators were determined. The highest PFAS concentrations were found in the liver [215.97 ng/g dry weight (dw)] of Chinese toads, followed by gonads (135.42 ng/g dw) and intestine (114.08 ng/g dw). A similar tissue distribution profile was found between legacy and emerging PFAS in the toads, and the occurrence of two emerging PFAS, 2,3,3,3-tetrafluoro-2-propanoate (HFPO-DA) and 6:2 hydrogen-substituted polyfluorooctane ether sulfonate (6:2 H-PFESA) in the amphibians were for the first time reported. Field-based bioaccumulation factors of HFPO-DA were higher than perfluorooctanoic acid, indicating the higher bioaccumulation potential of this emerging PFAS than the legacy C8 compound. Males had significantly higher gonad PFAS levels than females while estradiol levels in gonads increased with increasing concentrations of certain PFAS (e.g., 6:2 H-PFESA), implying that PFAS may trigger estrogenic effects in the toads, especially for male toads.
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Ácidos Alcanossulfônicos , Fluorocarbonos , Poluentes Químicos da Água , Feminino , Masculino , Animais , Bioacumulação , Lagos , Distribuição Tecidual , Baías , Fluorocarbonos/análise , China , Poluentes Químicos da Água/análise , Bufonidae , Monitoramento AmbientalRESUMO
Halogenated flame retardants (HFRs) are a large class of chemical additives intended to meet flammability safety requirements, and at present, they are ubiquitous in the environment. Herein, we conducted the target analysis and suspect screening of legacy and novel HFRs and their metabolites in the blubber of finless porpoises (Neophocaena phocaenoides; n = 70) and Indo-Pacific humpback dolphins (Sousa chinensis; n = 35) stranded in Hong Kong, a coastal city in the South China Sea, between 2013 and 2020. The average concentrations of total target HFRs (ΣHFRs) were 6.48 × 103 ± 1.01 × 104 and 1.40 × 104 ± 1.51 × 104 ng/g lipid weight in porpoises and dolphins, respectively. Significant decreasing temporal trends were observed in the concentrations of tetra-/penta-/hexa-bromodiphenyl ethers (tetra-/penta-/hexa-BDEs) in adult porpoises stranded from 2013-2015 to 2016-2020 (p < 0.05), probably because of their phasing out in China. No significant difference was found for the concentrations of decabromodiphenyl ether and hexabromocyclododecane, possibly due to their exemption from the ban in China until 2025 and 2021, respectively. Eight brominated compounds were additionally identified via suspect screening. A positive correlation was found between the concentrations of tetra-BDE and methyl-methoxy-tetra-BDE (Me-MeO-tetra-BDE) (p < 0.05), indicating that the metabolism of tetra-BDE may be a potential source of Me-MeO-tetra-BDE in marine mammals.
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Golfinhos , Retardadores de Chama , Toninhas , Animais , Hong Kong , Retardadores de Chama/análise , Toninhas/metabolismo , Golfinhos/metabolismo , China , Éteres Difenil Halogenados/análise , Monitoramento Ambiental/métodosRESUMO
Growing application of zinc oxide nanoparticles (ZnO-NPs) in global market has led to the concern over their potential environmental impacts. Filter feeders like mussels are prone to nanoparticles due to their superior filter-feeding ability. Temperature and salinity of coastal and estuarine seawaters often vary seasonally and spatially, and their changes may jointly influence physicochemical properties of ZnO-NPs and thus their toxicity. This study, therefore, aimed to investigate the interactive effect of temperatures (15, 25 and 30 °C) and salinities (12 and 32 PSU) on physicochemical properties and sublethal toxicity of ZnO-NPs towards a marine mussel Xenostrobus securis, and to compare that with the toxicity caused by Zn2+ ions (zinc sulphate heptahydrate). The results revealed increased particle agglomeration but decreased zinc ion release of ZnO-NPs at the highest temperature and salinity condition (30 °C and 32 PSU). After exposure, ZnO-NPs significantly reduced survival, byssal attachment rate and filtration rate of the mussels at high temperature and salinity (30 °C and 32 PSU). Glutathione S-transferase and superoxide dismutase activities in the mussels were suppressed at 30 °C. These aligned with the augmented zinc accumulation with increasing temperature and salinity which could likely be attributable to increased particle agglomeration of ZnO-NP and enhanced intrinsic filtration rate of the mussels under these conditions. Together with the observed lower toxic potency of Zn2+ compared to ZnO-NPs, our results suggested that the mussels might accumulate more zinc through particle filtration under higher temperature and salinity, eventually resulting in elevated toxicity of ZnO-NPs. Overall, this study demonstrated the necessity to consider the interactive effect of environmental factors such as temperature and salinity during the toxicity assessment of nanoparticles.
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Nanopartículas Metálicas , Mytilidae , Nanopartículas , Óxido de Zinco , Animais , Óxido de Zinco/toxicidade , Temperatura , Salinidade , Nanopartículas/toxicidade , Zinco , Nanopartículas Metálicas/toxicidadeRESUMO
Biochar-derived dissolved black carbon (DBC) molecules are dependent on the BC formation temperature and affect the fate of emerging contaminants in waters, such as polyvinyl chloride microplastic (MPPVC). However, the temperature-dependent evolution and MPPVC-interaction of DBC molecules remain unclear. Herein, we propose a novel DBC-MPPVC interaction mechanism by systematically interpreting heterogeneous correlations, sequential responses, and synergistic relationships of thousands of molecules and their linking functional groups. Two-dimensional correlation spectroscopy was proposed to combine Fourier transform-ion cyclotron resonance mass spectrometry and spectroscopic datasets. Increased temperature caused diverse DBC molecules and fluorophores, accompanied by molecular transformation from saturation/reduction to unsaturation/oxidation with high carbon oxidation states, especially for molecules with acidic functional groups. The temperature response of DBC molecules detected via negative-/positive-ion electrospray ionization sequentially occurred in unsaturated hydrocarbons â lignin-like â condensed aromatic â lipid-/aliphatic-/peptide-like â tannin-like â carbohydrate-like molecules. DBC molecular changes induced by temperature and MPPVC interaction were closely coordinated, with lignin-like molecules contributing the most to the interaction. Functional groups in DBC molecules with m/z < 500 showed a sequential MPPVC-interaction response of phenol/aromatic ether C-O, alkene CâC/amide CâO â polysaccharides C-O â alcohol/ether/carbohydrate C-O groups. These findings help to elucidate the critical role of DBCs in MP environmental behaviors.
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Microplásticos , Plásticos , Cloreto de Polivinila , Lignina , Temperatura , Carbono , Fuligem , Éteres , CarboidratosRESUMO
There is growing contamination of copper (Cu) in the marine environment, particularly after the ban of organotin compounds and the increase of the use of Cu-based antifouling paints. Although there are increasing research interests in temperature-dependent chemical toxicity to aquatic organisms, most existing studies focused on acute impacts of chemicals at high concentrations. This study aimed to investigate the interacting effect of temperature and copper exposure at environmentally relevant concentrations on survival and development in the marine copepod Tigriopus japonicus with a partial life-cycle toxicity test. Expressions of five stress response genes in the copepod, namely two glutathione S-transferases (GST-S and GST-O), two heat shock proteins (HSP70 and HSP90), and glutathione reductase (GR) were also investigated. The copepod's survival was significantly impaired at 15 °C after development to adult stage, while its developmental time reduced significantly with increasing temperature. Copper at the two environmentally relevant test concentrations had no significant impacts on these apical endpoints whereas the interaction between Cu and temperature was more significant in modulating gene expressions. GST-S, GST-O and HSP90 genes in copepods exposed to 100 µg Cu L-1 were significantly upregulated at 20 °C. At 32 °C, most genes were either insignificantly expressed or down-regulated, compared to the control, likely suggesting that thermal stress inhibited the copepod's antioxidative defense system. Overall, the results revealed that the joint Cu and thermal stresses have significantly elicited antioxidative system in the copepods. It clearly demonstrated the need for more fundamental studies about potential impacts of different environmental factors such as temperature on chemical toxicity under realistic scenario of marine pollution.
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Copépodes , Poluentes Químicos da Água , Animais , Cobre/toxicidade , Cobre/metabolismo , Copépodes/genética , Temperatura , Testes de Toxicidade/métodos , Antioxidantes/metabolismo , Poluentes Químicos da Água/toxicidade , Poluentes Químicos da Água/metabolismoRESUMO
Microplastics (MPs) and nanoplastics (NPs) are widely detected in food and the human environment. More studies have begun to pay attention to the influence of MPs and NPs on genetics; in particular, exposure of paternal generation to MPs and NPs on epigenetic inheritance and the offspring of animal models have attracted considerable interest. In this Viewpoint, we mainly discuss the suggestion that reproductive genetic changes in the male parent have the potential to be transferred to the offspring and illustrate how MPs and NPs in the father tissues are distributed in later generations. We provide a systematic understanding of the potential health hazards of paternal exposure to MPs and NPs to subsequent generations and put forth recommendations about the epigenetic effects for future research on public health and food safety.
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Microplásticos , Poluentes Químicos da Água , Animais , Masculino , Humanos , Microplásticos/toxicidade , Plásticos/toxicidade , Herança Paterna , Reprodução , Modelos Animais , Poluentes Químicos da Água/análiseRESUMO
Emerging contaminants such as nanoplastics and nanoparticles likely experience similar environmental behaviours, fate and effects but our knowledge of their combined toxicity is scanty. This study, therefore, investigated the joint toxicity of polystyrene nanoplastics (PNPs) and zinc oxide nanoparticles (ZnO-NPs) to an ecologically important rotifer Brachionus koreanus, and compared with the joint toxicity of PNPs and Zn ions (Zn-IONs from ZnSO4·7H2O). With increasing concentration, ZnO-NPs formed significant agglomeration with PNPs for up to 1.3 times of the original hydrodynamic size of ZnO-NPs, alongside doubling in their sedimentation and thereby losing 58% of their released Zn ions. In contrast, the availability of Zn-IONs was less affected by the agglomeration and sedimentation of PNPs, with only a loss of 18% of Zn ions at the highest concentration of PNPs. Consequently, as suggested by Concentration Addition and Independent Action models and the Model Deviation Ratios, ZnO-NPs and PNPs exerted an antagonistic interaction whereas Zn-IONs and PNPs exhibited an additive effect. We also advocate the use of the Nonparametric Response Surface method, which is more useful to predict the toxicity of chemical mixtures with interacting effects. Our findings suggested a potential difference between particle-particle and particle-ion interactions, especially at higher test concentrations, which may eventually affect their toxicity. We, therefore, call for a more systematic evaluation of commonly coexisting chemical mixtures which consist of nanoplastics and manufactured nanomaterials.
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Nanopartículas , Poluentes Químicos da Água , Óxido de Zinco , Óxido de Zinco/toxicidade , Zinco/toxicidade , Zinco/análise , Poliestirenos/toxicidade , Microplásticos , Poluentes Químicos da Água/toxicidade , Nanopartículas/toxicidade , ÍonsRESUMO
Parkinson's disease (PD) is characterized by dopaminergic neurodegeneration in nigrostriatal and cortical brain regions associated with pathogenic α-synuclein (αSyn) aggregate/oligomer accumulation. LRRK2 hyperactivity is a disease-modifying therapeutic target in PD. However, LRRK2 inhibition may be associated with peripheral effects, albeit with unclear clinical consequences. Here, we significantly reduced αSyn oligomer accumulation in mouse striatum through long-term LRRK2 inhibition using GNE-7915 (specific brain-penetrant LRRK2 inhibitor) without causing adverse peripheral effects. GNE-7915 concentrations in wild-type (WT) mouse sera and brain samples reached a peak at 1 h, which gradually decreased over 24 h following a single subcutaneous (100 mg/kg) injection. The same dose in young WT and LRRK2R1441G mutant mice significantly inhibited LRRK2 kinase activity (Thr73-Rab10 and Ser106-Rab12 phosphorylation) in the lung, which dissipated by 72 h post-injection. 14-month-old mutant mice injected with GNE-7915 twice weekly for 18 weeks (equivalent to ~13 human years) exhibited reduced striatal αSyn oligomer and cortical pSer129-αSyn levels, correlating with inhibition of LRRK2 hyperactivity in brain and lung to WT levels. No GNE-7915-treated mice showed increased mortality or morbidity. Unlike reports of abnormalities in lung and kidney at acute high doses of LRRK2 inhibitors, our GNE-7915-treated mice did not exhibit swollen lamellar bodies in type II pneumocytes or abnormal vacuolation in the kidney. Functional and histopathological assessments of lung, kidney and liver, including whole-body plethysmography, urinary albumin-creatinine ratio (ACR), serum alanine aminotransferase (ALT) and serum interleukin-6 (inflammatory marker) did not reveal abnormalities after long-term GNE-7915 treatment. Long-term inhibition of mutant LRRK2 hyper-kinase activity to physiological levels presents an efficacious and safe disease-modifying therapy to ameliorate synucleinopathy in PD.
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Atmospheric particulate matter, especially in urban and industrial environments, can act as a source of different organic pollutants that can pose significant health impacts to residents. However, the pollution status and transport mechanisms of fine particle-bound polycyclic aromatic hydrocarbons (PAHs) in indoor and outdoor environments are uncertain. This study aimed to determine the spatial distribution and morphological characteristics of fine particle-bound PAHs and analyze the factors (source contributions and backward trajectories) that influence their concentrations. The results showed that mean concentrations of 16 PAHs were higher in indoor dust as compared to outdoor dust. In addition, the lowest concentrations of the 16 PAHs were found on the 11-20th floor, with smoking households > nonsmoking households (except Nap, Acy, and Ace). The 2-3 ring PAHs were more prominent in households with cooking activities. The particle size distribution showed that most of the particles were <62 µm in diameter, indicating that the indoor particles were smaller in size. Furthermore, the range of δ13C values in the outdoor dust (-30.17 ~ -28.63 ) samples was significantly lower than in indoor dust (-28.29 ~ -22.53 ). The results based on diagnostic ratios, positive matrix factorization (PMF) analysis and backward trajectory model analysis suggested that the sources of PAHs in indoor and outdoor dust were mixed, originated both locally and from neighboring provinces transported over long distances, especially concentrated in the Yangtze River Delta area. Finally, carcinogenic risk values for indoor dust were greater than those for outdoor dust. Therefore, it is recommended that local governments and industries with high PAH emissions should implement proper protocols to monitor and minimize the pollution levels of PAHs in the urban industrial environment in order to mitigate their health risks.
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Poluentes Atmosféricos , Poluição do Ar em Ambientes Fechados , Poluentes Ambientais , Hidrocarbonetos Policíclicos Aromáticos , Poluentes Atmosféricos/análise , Poluição do Ar em Ambientes Fechados/análise , China , Poeira/análise , Monitoramento Ambiental/métodos , Poluentes Ambientais/análise , Material Particulado/análise , Hidrocarbonetos Policíclicos Aromáticos/análise , Medição de RiscoRESUMO
Excessive intake of retinoic acids (RAs) and the oxidative metabolites, 4-oxo-RAs, can lead to abnormal morphological development in animals. This study investigated spatiotemporal variations of concentrations and compositions of these compounds in Hong Kong's seawater and during algal blooms. Total concentrations of the studied compounds in seawater were up to 0.790 and 0.427 ng/L in dry and wet seasons, respectively, though no significant seasonal variation was observed. Spatially, the Deep Bay Water Control Zone was the most enriched area with the studied compounds owing to its semi-enclosed nature and influence from the Pearl River discharge. During algal blooms, the studied compounds were detected up to 4.74 ng/L. Based on calculated risk quotients, the ecological risk of the studied compounds to Hong Kong's marine ecosystems was low. Nevertheless, the occurrence and distribution of these chemicals in the marine environment should be closely monitored where algal blooms frequently occur.
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Ecossistema , Monitoramento Ambiental , Animais , Hong Kong , Rios , Água do Mar/químicaRESUMO
Volatile organic compound (VOC) emissions from pyrolysis of widely used biomass are expected to increase significantly under the carbon neutrality target. However, the dynamic emissions and evolution mechanism of biomass-VOCs remain unclear, hindered by complex reactions and offline measurements. Here, we propose a novel covariant evolution mechanism to interpret the emission heterogeneities, sequential temperature responses, and evolved correlations of both VOCs and residual functional groups (RFGs) during corn straw (CS), wood pellet (WP), and semibituminous coal (SBC) pyrolysis. An innovative combination of online thermogravimetric-Fourier transform infrared-gas chromatography/mass spectrometry and two dimensional-correlation spectroscopy was applied. The relative percentages of CS/WP-VOCs were higher than those of SBC-VOCs, and most VOCs tended to have relatively small carbon skeletons as the average carbon oxidation state increased. With the temperature increased from low to high during CS/WP pyrolysis, the primary sequential response of VOCs (acids â phenols/esters â alcohols/ethers/aldehydes/ketones â hydrocarbons/aromatics) corresponded to the RFG response (hydroxyl groups â -CH3/-CH2-/-CH groups â aliphatic ethers and conjugated ketones). Compared with the relative regularity for CS/WP responses, the gas-solid products from SBC pyrolysis exhibited complex temperature-dependent responses and high oxidation-induced variability. These insights provide favorable strategies for the online monitoring system to facilitate priority removal of coal and biomass fuels-VOCs.
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Carvão Mineral , Compostos Orgânicos Voláteis , Biomassa , Carbono , Éteres/análise , Cromatografia Gasosa-Espectrometria de Massas , Cetonas , Pirólise , Zea maysRESUMO
Being a class of vitamin A's main derivatives, retinoic acids (RAs) are important to animals' growth and development. Previous studies demonstrated that exposure of excessive amounts of RAs would lead to malformation and abnormal development in aquatic animals such as amphibians and fishes. Currently, there are only limited toxicity data of RAs available for freshwater species, while those for marine species are seriously lacking. This study aimed to fill such data gap by conducting toxicity tests on six marine species (i.e., one microalga, four invertebrates and one fish) towards the exposure to all-trans-RA (at-RA), which is the most widely distributed RA in the environment. Results showed that the embryo of medaka fish Oryzias melastigma was the most sensitive towards the exposure of at-RA while the gastropod Monodonta labio was the least sensitive. A species sensitivity distribution (SSD) was constructed based on the experimental results generated from the present study. An interim marine-specific predicted no-effect concentration (PNEC) of at-RA was derived at 2300 ng/L. By computing the hazard quotients using the interim marine-specific PNEC and available measured and predicted concentrations of RAs, we found the current levels of RAs posed no immediate risks to the marine environment of Hong Kong. The interim marine-specific PNEC was more than 500-fold of freshwater-specific PNEC (i.e., 3.93 ng/L), indicating that marine species were generally less sensitive than their freshwater counterparts towards RAs. This was the first study to document the concentration-response of various marine species towards at-RA exposure and construct the marine-specific SSD for assessing the ecological risk of at-RA towards the marine environment. Since various forms of RAs and their metabolites often coexist in aquatic environments, further studies should investigate their combined toxicity to an array of marine species of different trophic levels with consideration of chronic exposure scenarios.
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Oryzias , Poluentes Químicos da Água , Animais , Água Doce , Invertebrados , Tretinoína , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidadeRESUMO
Wastewater treatment plants (WWTPs) are important sources of antibiotic resistance genes (ARGs). Increasing attention has been paid to extracellular ARGs in cell-free form due to their horizontal gene transfer via transformation. However, the fate of the adsorbed form of extracellular ARGs that exist in extracellular polymeric substances (EPS) of activated sludge in WWTP remains largely unknown. Herein, seven EPS-associated ARGs along the anaerobic-anoxic-aerobic (AAO) process were quantified using quantitative polymerase chain reaction. Results show that the absolute abundances of EPS-associated ARGs were 0.69-4.52 logs higher than those of cell-free ARGs. There was no significant difference in the abundances of EPS-associated ARGs along the AAO process. Among these target genes, the abundances of EPS-associated sul genes were higher than those of EPS-associated tet and bla genes. Proteobacteria and Bacteroidetes were identified as the major secretors of EPS-associated ARGs, and they may play an important role in the proliferation of extracellular ARGs. Moreover, the transformation efficiencies of EPS-associated ARGs were 3.55-4.65 logs higher than those of cell-free ARGs, indicating that EPS-associated ARGs have higher environmental risks. These findings have advanced our understanding of EPS-associated ARGs and are useful for the control and risk assessment of ARGs in WWTPs.
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Matriz Extracelular de Substâncias Poliméricas , Esgotos , Antibacterianos/farmacologia , Resistência Microbiana a Medicamentos/genética , Genes Bacterianos , Águas ResiduáriasRESUMO
The coronavirus disease 2019 (COVID-19) pandemic is challenging the current public health emergency response systems (PHERSs) of many countries. Although environmental factors, such as those influencing the survival of viruses and their transmission between species including humans, play important roles in PHERSs, little attention has been given to these factors. This study describes and elucidates the roles of environmental factors in future PHERSs. To improve countries' capability to respond to public health emergencies associated with viral infections such as the COVID-19 pandemic, a number of environmental factors should be considered before, during, and after the responses to such emergencies. More specifically, to prevent pandemic outbreaks, we should strengthen environmental and wildlife protection, conduct detailed viral surveillance in animals and hotspots, and improve early-warning systems. During the pandemic, we must study the impacts of environmental factors on viral behaviors, develop control measures to minimize secondary environmental risks, and conduct timely assessments of viral risks and secondary environmental effects with a view to reducing the impacts of the pandemic on human health and on ecosystems. After the pandemic, we should further strengthen surveillance for viruses and the prevention of viral spread, maintain control measures for minimizing secondary environmental risks, develop our capability to scientifically predict pandemics and resurgences, and prepare for the next unexpected resurgence. Meanwhile, we should restore the normal life and production of the public based on the "One Health" concept, that views global human and environmental health as inextricably linked. Our recommendations are essential for improving nations' capability to respond to global public health emergencies.
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Sewage treatment plants (STPs) are important in densely populated megacities like Hong Kong to control the release of harmful pollutants from households and industries into the receiving water bodies and maintain water quality for supporting various beneficial uses. This study investigated the occurrence of the teratogenic retinoic acids (RAs) and their oxidative metabolites in sewage and sludge of six selected STPs that treat about 87% of all sewage in Hong Kong annually, and compared the removal efficiencies of these compounds from sewage between two major sewage treatment processes, i.e., chemically enhanced primary treatment (CEPT) and secondary (biological) treatment. The total concentrations of the studied RAs in influent, effluent and sludge from the six selected STPs were found between 21.5 and 33.1 ng/L, 12.0-20.4 ng/L, and 4.33-7.02 ng/g dry weight, respectively. The compounds were dominated by all-trans-RA and 13-cis-RA, together accounting for 46.9-65.6%, 38.4-56.7%, and 62.8-82.8% of the total RAs in influent, effluent and sludge, respectively. The studied RAs could not be satisfactorily removed by both treatment processes with removal efficiencies ranging from 25.4% to 47.4% only, without significant difference in their removal between CEPT and secondary treatment. Based on the calculated hazard quotients of all-trans-RA equivalents (0.248-0.521), the treated effluents from all the six STPs exhibited medium ecological risks to the receiving coastal environment. Therefore, continuous monitoring of these compounds and enhancement of treatment technologies of STPs shall be considered in the future to improve the removal efficiencies of these compounds.