[Clinical observation of virtual reality technology combined with isokinetic strength training for patients after anterior cruciate ligament reconstruction].

OBJECTIVE: To explore application value and effectiveness of virtual reality technology combined with isokinetic muscle strength training in the rehabilitation of patients after anterior cruciate ligament (ACL) reconstruction surgery. METHODS: Forty patients who underwent ACL reconstruction surgery from December 2021 to January 2023 were selected and divided into control group and observation group according to treatment methods, 20 patients in each group. Control group was received routine rehabilitation training combined with isokinetic muscle strength training, including 15 males and 5 females, aged from 17 to 44 years old, with an average of (29.10±8.60) years old. Observation group was performed virtual reality technology combined with isokinetic muscle strength training, including 16 males and 4 females, aged from 17 to 45 years old with an average of (30.95±9.11) years old. Lysholm knee joint score, knee extension peak torque, and knee flexion peak torque between two groups at 12 (before training) and 16 weeks (after training) after surgery were compared. RESULTS: All patients were followed up for 1 to 6 months with an average of (3.30±1.42) months. There were no statistically significant difference in Lysholm knee joint score, peak knee extension peak torque, and peak knee flexion peak torque between two groups (P>0.05) before training. After training, Lysholm knee joint score, knee extension peak torque, and knee flexion peak torque of both groups were improved compared to before training (P<0.05);there were significant difference in Lysholm knee joint score, knee extension peak torque, and knee flexion peak torque between two groups(P<0.05). CONCLUSION: The application of virtual reality technology combined with isokinetic muscle strength training could promote recovery of knee joint function and enhance muscle strength in patients after ACL reconstruction surgery in further.

[Analysis and Enlightenment of Foreign Open Breast Image Datasets].

With the upsurge of medical artificial intelligence,the use of computer vision technology to study medical images,which can effectively help doctors to identify and screen diseases,has become a focus of researchers.This paper summarizes the basic situation,specific information,related research,and data sharing and utilizing ways of foreign breast image datasets.This review provides inspirations for the opening of Chinese medical and health data.

Effects of Conservation Tillage on Soil Physicochemical Properties and Crop Yield in an Arid Loess Plateau, China.

Conservation tillage can improve soil physical structure and water storage, protect moisture, and increase crop yield. However, the long-term adoption of a single tillage method may have some adverse effects on soil and ecological environment, although crop yields have increased. Through informed allocation of soil tillage techniques, the combination and configuration of soil tillage measures, such as rotary tillage, subsoiling, and no tillage may reduce the shortcomings of traditional long-term farming. To explore the long-term production mode suitable for production of maize in the loess dryland area, a long-term experiment was conducted in Fuping County, Shaanxi Province, from 2013 to 2018. Six farming modes were used in the experiment: no tillage/subsoiling (N ↔ S), subsoiling/rotary tillage (S ↔ R), rotary tillage/no tillage (R ↔ N), continuous no tillage (N ↔ N), continuous subsoiling (S ↔ S), and continuous rotary tillage (R ↔ R). The changes in soil physical and chemical properties, soil water use patterns, soil water storage, conservation effects during the fallow and growth period, and the effects on farmland yield increase were analyzed. The results showed that rotary tillage can effectively improved soil structure and reduced soil bulk density, where N ↔ S treatment soil bulk density is low and in 0-60 cm soil layer averaged 1.31 g/cm3. Different tillage treatments could be used during the fallow period to store additional soil moisture: the N ↔ S treatment showed good water storage effect. Compared to traditional tillage, different tillage methods provided better soil moisture conditions for crops during the growth period, where N ↔ S treatment showed good soil moisture status during the growth period of spring maize. Among all the treatments, N ↔ S treatment effectively increased the organic carbon storage in the 0-60 cm soil layer, which was 54.3 t/hm2. Compared with traditional tillage, different tillage treatments effectively increased plant height and dry matter accumulation of spring maize, where N ↔ S treatment was found to be the best. Compared with the traditional rotary tillage model, the N ↔ S treatment significantly increased crop yield and water use efficiency (WUE) in continuous cropping fields of corn, the average yield of spring corn was 9340.2 kg/hm2, and the average WUE was 22.9 kg/(hm2·mm). In summary, for long-term sustainable development, the N ↔ S model is the best rotational tillage mode for continuous maize cropping in loess soil.

Complete mitochondrial genome of banana new pest Basilepta fulvipes (Coleoptera: Eumolpinae) and phylogenetic analysis.

Basilepta fulvipes (Motschulsky, 1860) is a banana new pest and mainly distributed in Eastern Asia. The complete mitogenome of B. fulvipes (GenBank accession number MT627597) is 15,762 bp in size, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a noncoding D-loop region. The D-loop region is located between 12S rRNA and tRNAIle . The base composition of the whole B. fulvipes mitogenome is 41.66% for A, 8.89% for G, 34.32% for T and 15.12% for C, with a high AT bias of 75.98%. The present data could contribute to further detailed phylogeographic analysis and comprehensive control of this banaba new pest.

Enhancement of the second plateau in solid high-order harmonic spectra by the two-color fields.

We theoretically investigate high-order harmonic generation (HHG) from solids in two-color fields. It is found that under the premise of maintaining the same amplitude, the intensity of the second plateau can be enhanced by two to three orders in a proper two-color field compared with the result in the monochromatic field with the same frequency as the driving pulse of the two-color field. This can be attributed to the fact that most excited electrons can be driven to the top of the first conduction band due to the larger vector potential of the two-color fields, which leads to the higher electron population of upper conduction bands. Moreover, we also find that isolated attosecond pulses can be generated from solids by choosing a proper two-color field that allows the electrons to reach the top of the first conduction band only once. This work provides a promising method for extending the range of solid HHG spectra in experiments.

Genome-wide analysis of microsatellite sequence in seven filamentous fungi.

Abundance of microsatellites with repeated unit lengths of 1-6 base pairs in seven fungi: Aspergillus nidulans, Coprinus cinereus, Cryptococcus neoformans (serotype A), Fusarium graminearum, Magnaporthe grisea, Neurospora crassa and Ustilago maydis were investigated on genomic scale. The results showed that each species has its specific profile for different types and different motifs of SSR loci. Ascomycetes fungi M. grisea, N. crassa and basidiomycete fungus U. maydis adopt much more microsatellites than other fungi examined. Total amount of 15,751, 14,788 and 6,854 SSR loci were observed respectively, average density is 406, 389 and 347 per Mbp sequence; overall length of SSR sequence was 0.82%, 0.95% and 0.79% of genomic sequence respectively. While ascomycetes fungus F. graminearum and A. nidulans contains the least SSRs in the genomic DNA, only 4,679 and 4,837 tracts were observed in 36 Mb and 30 Mb genomic sequence respectively. Microsatellite repeats in protein coding regions are investigated in Aspergillus nidulans, Magnaporthe grisea, and Neurospora crassa also, the results show that the difference of different types and motifs among three fungi is very little than that in whole genomic sequence. For trinucleotide repeats, overrepresent (comparing to the total base pair of protein coding region) of AGC, GGC, AGG, ACG and ACC was observed in coding region, frequencies of AAC and AAG were not difference between coding and non-coding region, AAT, AGT and ATG were underrepresent in coding region excepted for A. nidulans, in which ATG was overrepresentative.

[Analysis of the secreted proteins encoded by genes in genome of filamental fungus (Neurospora crassa)].

The completed genome sequence of filamental fungus Neurospora crassa has completed and 10,082 open reading frames (ORFs) were predicted in whole genome. Among them, 437 proteins were secreted ones based on the combination of prediction algorithms SignalP v3.0 and PSORT, transmembrane domains prediction algorithms TMHMM v2.0 and THUMBUP, potential GPI-anchor sites prediction algorithm big-PI predictor and the subcellular protein location prediction algorithm TargetP v1.01. The minimum ORF length of the encoding sequences for 437 secreted proteins was 252 bp, the maximum ORF length was 6 604 bp and average was 1 433 bp. The length of signal peptide of secreted proteins ranged from 15 to 59 amino acids. Among the 437 secreted proteins, 205 were provided with function description. The function of secretory proteins were involved in kinds of enzyme, cell energy, transmission, cell recovered itself and defense mechanism et al. These results implied that processes catalyzed by these proteins probably occurred outside of cells, at least partially.

[Frequency and distribution of variable-number tandem repeats in the genome of Aspergillus niculans].

A total of 30.1 Mb of publicly available DNA sequence in Aspergillus niculans was researched for mono- to hexanucleotide variable-number tandem repeat (VNTR) to determine its type, size and frequency. A total of 4 837 VNTRs were observed in whole genomic DNA sequence with criteria of VNTR length > 15 bp and 80% matches. Considering all six classes of VNTRs, they occur on average about once every 6.2 kb for mono- to hexanucleotide in genomic DNA. The most abundance variable-number tandem repeat is pentanucleotide repeats; the number was 1 386, followed by hexanucleotide and trinucleotide repeats, the number was 1 228 and 1 199 respectively. The least abundance is dinucleotide repeat, only 144 tracts.