RESUMO
Incomplete pattern clustering is a challenging task because the unknown attributes of the missing data introduce uncertain information that affects the accuracy of the results. In addition, the clustering method based on the single view ignores the complementary information from multiple views. Therefore, a new belief two-level weighted clustering method based on multiview fusion (BTC-MV) is proposed to deal with incomplete patterns. Initially, the BTC-MV method estimates the missing data by an attribute-level weighted imputation method with k-nearest neighbor (KNN) strategy based on multiple views. The unknown attributes are replaced by the average of the KNN. Then, the clustering method based on multiple views is proposed for a complete data set with estimations; the view weights represent the reliability of the evidence from different source spaces. The membership values from multiple views, which indicate the probability of the pattern belonging to different categories, reduce the risk of misclustering. Finally, a view-level weighted fusion strategy based on the belief function theory is proposed to integrate the membership values from different source spaces, which improves the accuracy of the clustering task. To validate the performance of the BTC-MV method, extensive experiments are conducted to compare with classical methods, such as MI-KM, MI-KMVC, KNNI-FCM, and KNNI-MFCM. Results on six UCI data sets show that the error rate of the BTC-MV method is lower than that of the other methods. Therefore, it can be concluded that the BTC-MV method has superior performance in dealing with incomplete patterns.
Assuntos
Algoritmos , Reprodutibilidade dos Testes , Análise por ConglomeradosRESUMO
FBW7 (F-box and WD repeat domain-containing 7), also known as CDC4, AGO and SEL10, is the substrate recognition component of an evolutionary conserved SCF (complex of SKP1, CUL1 and F-box protein)-type E3 ubiquitin ligase. It is a recognized tumor suppressor because it targets multiple oncoproteins for ubiquitination-mediated destruction and its mutations are frequently identified in a variety of human malignancies. However, the function of FBW7 in proliferation of cholangiocarcinoma (CCA) remains unknown. We found that overexpression of FBW7α induced CCA cell arrest in G1 phase of cell cycle and inhibited cell proliferation in vitro and CCA xenograft tumor growth, suggesting that FBW7α is a tumor suppressor in CCA progression. Overxpression of FBW7α resulted in the protein degradation of its substrates such as c-Myc and cyclin E which promote CCA cell proliferation. Restoration of the expression of c-Myc, but not cyclin E, rescued the proliferation of FBW7α-overexpression CCA cells. These results suggest that FBW7α plays an essential inhibitory role in CCA progression, indicating that targeting FBW7α substrate c-Myc may be a potential strategy for CCA treatment.
Assuntos
Neoplasias dos Ductos Biliares/prevenção & controle , Ductos Biliares Intra-Hepáticos/patologia , Proteínas de Ciclo Celular/metabolismo , Proliferação de Células , Colangiocarcinoma/prevenção & controle , Ciclina E/metabolismo , Proteínas F-Box/metabolismo , Proteínas Oncogênicas/metabolismo , Proteínas Proto-Oncogênicas c-myc/metabolismo , Ubiquitina-Proteína Ligases/metabolismo , Animais , Apoptose , Neoplasias dos Ductos Biliares/genética , Neoplasias dos Ductos Biliares/metabolismo , Neoplasias dos Ductos Biliares/patologia , Ductos Biliares Intra-Hepáticos/metabolismo , Western Blotting , Ciclo Celular , Proteínas de Ciclo Celular/genética , Colangiocarcinoma/genética , Colangiocarcinoma/metabolismo , Colangiocarcinoma/patologia , Ciclina E/genética , Proteínas F-Box/genética , Proteína 7 com Repetições F-Box-WD , Humanos , Técnicas Imunoenzimáticas , Masculino , Camundongos , Camundongos Nus , Proteínas Oncogênicas/genética , Proteínas Proto-Oncogênicas c-myc/genética , RNA Mensageiro/genética , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Células Tumorais Cultivadas , Ubiquitina-Proteína Ligases/genética , Ensaios Antitumorais Modelo de XenoenxertoRESUMO
OBJECTIVE: To analyze the characteristic of chronic paranasal sinusitis CT scans and discuss the relationship between the anatomic variations in ostiomeatal complex (OMC) and the incidence of chronic sinusitis. METHOD: One hundred and ninety-seven cases diagnosis as sinusitis were selected and analyzed,whose age ranged between 4 and 74 years old. Several common anatomical abnormalities in ostiomeatal complex were evaluated through the observation of the coronal and axial plane CT scans of the paranasal sinusitis. RESULT: The incidence of anatomic variations in OMC concentrated in the age between 11-20. The incidence of chronic sinusitis also concentrated in the age between 11-20. Deviation of nasal septum, uncinate variations, inferior-turbinate hypertrophy were observed between the chronic sinusitis group and the none chronic sinusitis group,the correlation between deviation of nasal septum, uncinate variations, inferior-turbinate hypertrophy and chronic sinusitis were significant (P<0.05). CONCLUSION: The occurrence of OMC variation is common. The anatomic variations may be one of the causes of chronic paranasal sinusitis, especially deviation of nasal septum, uncinate variations and inferior-turbinate hypertrophy. If these abnormalities were altered,it will have a great significance for the preservation and treatment of chronic paranasal sinusitis.