The Effect of Anti-fatigue Decoction on the Behaviors and Serological Indicators in a Central Fatigue Rat Model.

This study aimed to assess the effects of Anti-fatigue Decoction (AFD) against central fatigue by observing the behaviors and serological indicators of rats modeled by the modified multiple platform method (MMPM) after drug intervention. Grip strength measurements were used to evaluate the muscle strength of rats. The open field test was utilized to assess anxiety-like behavior, while the Morris water maze test was conducted to evaluate the memory function of the rats. Following the behavioral assessments, rat serum samples were collected to measure the concentrations of corticosterone (CORT) and lactic acid (LAC). The concentration of LAC was determined using the colorimetric method, while the concentration of CORT was measured using the enzyme-linked immunosorbent assay (ELISA) method. Compared to the blank control group, following MMPM modeling, rats exhibited significant reductions in grip strength and impaired ability to memory. The serum analysis revealed increased levels of LAC and CORT in the model group rats. AFD can noticeably reverse these adverse changes to a certain extent. These findings highlight the positive effects of AFD and coenzymeQ10 on physical and cognitive abilities and alterations in serum biomarker levels of central fatigue rats.

Replicating human characteristics: A promising animal model of central fatigue.

Central fatigue is a common pathological state characterized by psychological loss of drive, lack of appetite, drowsiness, and decreased psychic alertness. The mechanism underlying central fatigue is still unclear, and there is no widely accepted successful animal model that fully represents human characteristics. We aimed to construct a more clinically relevant and comprehensive animal model of central fatigue. In this study, we utilized the Modified Multiple Platform Method (MMPM) combined with alternate-day fasting (ADF) to create the animal model. The model group rats are placed on a stationary water environment platform for sleep deprivation at a fixed time each day, and they were subjected to ADF treatment. On non-fasting days, the rats were allowed unrestricted access to food. This process was sustained over a period of 21 days. We evaluated the model using behavioral assessments such as open field test, elevated plus maze test, tail suspension test, Morris water maze test, grip strength test, and forced swimming test, as well as serum biochemical laboratory indices. Additionally, we conducted pathological observations of the hippocampus and quadriceps muscle tissues, transmission electron microscope observation of mitochondrial ultrastructure, and assessment of mitochondrial energy metabolism and oxidative stress-related markers. The results revealed that the model rats displayed emotional anomalies resembling symptoms of depression and anxiety, decreased exploratory behavior, decline in learning and memory function, and signs of skeletal muscle fatigue, successfully replicating human features of negative emotions, cognitive decline, and physical fatigue. Pathological damage and mitochondrial ultrastructural alterations were observed in the hippocampus and quadriceps muscle tissues, accompanied by abnormal mitochondrial energy metabolism and oxidative stress in the form of decreased ATP and increased ROS levels. In conclusion, our ADF+MMPM model comprehensively replicated the features of human central fatigue and is a promising platform for preclinical research. Furthermore, the pivotal role of mitochondrial energy metabolism and oxidative stress damage in the occurrence of central fatigue in the hippocampus and skeletal muscle tissues was corroborated.

Effects of upward flow rate and modified biochar location on the performance and microecology of an anaerobic reactor treating kitchen waste.

Increasing the value of food waste through anaerobic digestion is an attractive strategy. Meanwhile, the anaerobic digestion of kitchen waste also faces some technical challenges. In this study, four EGSB reactors were equipped with Fe-Mg-chitosan bagasse biochar at different locations, and the reflux pump flow rate was increased to change the upward flow rate of the reactor. The effects of adding modified biochar at different locations under different upward flow rate on the efficacy and microecology of anaerobic reactors treating kitchen waste were investigated. Results showed that Chloroflexi was the dominant microorganism when the modified biochar was added to the lower, middle, and upper parts of the reactor and mixed in the reactor, accounting for 54%, 56%, 58%, and 47%, respectively, on day 45. With the increased upward flow rate, the abundance of Bacteroidetes and Chloroflexi increased, while Proteobacteria and Firmicutes decreased. It was worth noting that the best COD removal effect was achieved when the anaerobic reactor upward flow rate was v2 = 0.6 m/h and the modified biochar was added in the upper part of the reactor, during which the average COD removal rate reached 96%. In addition, mixing modified biochar throughout the reactor while increasing the upward flow rate provided the greatest stimulus for the secretion of tryptophan and aromatic proteins in the sludge extracellular polymeric substances. The results provided a certain technical reference for improving the efficiency of anaerobic digestion of kitchen waste and scientific support for the application of modified biochar to the anaerobic digestion process.

Long-term effect of perfluorooctanoic acid on the anammox system based on metagenomics: Performance, sludge characteristic and microbial community dynamic.

The effects of PFOA on the nitrogen removal performance, microbial community and functional genes of anaerobic ammonium oxidation (anammox) sludge in an anaerobic baffled reactor (ABR) were investigated. The removal efficiencies of ammonia nitrogen (NH4+-N) and nitrite (NO2--N) decreased from 93.90 ± 3.64% and 98.6 ± 1.84% to 77.81 ± 6.86% and 77.96 ± 1.88% when PFOA increased from 5 mg/L to 50 mg/L, respectively. X-ray photoelectron spectra analysis of the anammox sludge showed the presence of both C-F and CaF2 forms of F. Metagenomics analysis of the anammox sludge in the first compartment illustrated that the relative abundance of Ca.Brocadia and Ca.Kuenenia decreased from 22.21% and 5.61% to 2.11% and 2.84% at 50 mg/L PFOA compared with that without PFOA. In addition, the nitrogen metabolism pathway showed that adding 50 mg/L PFOA decreased the expression of HzsB, HzsC, and Hdh (anammox genes) by 0.096%, 0.05% and 0.062%, respectively.

Metagenomic analysis revealed the microbiota and metabolic function during co-composting of food waste and residual sludge for nitrogen and phosphorus transformation.

This paper used bagasse as a composting additive and bulking agent in order to investigate the aerobic composting process of food waste and residual sludge. Accordingly, the variations of nitrogen and phosphorus nutrients, microbiota and metabolic function during the composting process were systematically explored. Three piles with residual sludge, food waste and bagasse mass ratios of 1:1:1, 2:1:1 and 4:1:1 were set. The ammonia nitrogen content in the three compost piles were 3.18 mg/g, 4.68 mg/g and 5.84 mg/g at the end of composting. The final available phosphorus content of the three piles were 3.42 mg/g, 6.70 mg/g and 11.21 mg/g, respectively. X-ray photoelectron spectroscopy (XPS) analysis showed that absorption peaks attributed to amines, amino acids and amides appeared in the 1:1:1 pile. Metagenomic analysis of the glycolysis and ammonia transformation pathways showed that the total relative abundance of key enzyme genes for the conversion of glucose to glucose-6-phosphate in the three plies were 0.326%, 0.213% and 0.248%, respectively. The total relative abundance of 2 glutamate dehydrogenase (GDH2), glud1-2 and E1,4,1,4 dehydrogenases in the three piles was 0.125%, 0.151% and 0.160%, respectively, as the main enzymes for the mutual conversion of ammonia and glutamate.