RESUMO
AIM: To study the cloning, expression and antigen of therapeutic multi-epitope gene of hepatitis B virus. METHODS: The multi-epitope gene of hepatitis B virus(BPT) was designed, synthesized and cloned into the prokaryotic expression vector pBAD/gIIIA. Then it was transformed into E.coli Top10 and multi-epitope protein of hepatitis B virus(B-BPT) was expressed under the induction of Arabinose. The immunogenicity of the protein was analyzed by Western blot detection. RESULTS: The recombinant plasmid pBAD/BPT was constructed successfully and the protein of multi-epitope gene of hepatitis B virus was expressed in E.coli. Western blot detection showed the protein had ideal antigenicity. CONCLUSION: The design of therapeutic multi-epitope gene of hepatitis B virus is proved to be correct. The expressed protein may be a good therapeutic vaccine.
Assuntos
Epitopos/imunologia , Epitopos/metabolismo , Vetores Genéticos/genética , Antígenos de Superfície da Hepatite B/imunologia , Antígenos de Superfície da Hepatite B/metabolismo , Vírus da Hepatite B/imunologia , Western Blotting , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Epitopos/genética , Antígenos de Superfície da Hepatite B/genética , Vacinas contra Hepatite B/genética , Vacinas contra Hepatite B/imunologia , Vacinas contra Hepatite B/metabolismo , Vírus da Hepatite B/genéticaRESUMO
AIM: To explore the effects of HBsAg-pulsed dendritic cells (DCs) on the proliferation and killing functions of cytokine-induced killer (CIK) cells. METHODS: The peripheral blood mononuclear cells (PBMCs) were isolated from the patients with chronic hepatitis B by the routine method, and induced into specific DCs with HBsAg pulsed. The (3)H-TdR incorporation method was used to determine the stimulation effect of HBsAg-pulsed DCs on the proliferation of CIK cells. LDH release assay was used to measure the specific killing activity of CIK cells on HepG2215 cells. RESULTS: The HBsAg-pulsed DCs could induce the memory proliferation of CIK cells and strengthen the killing activity of CIK cells (P<0.05). CONCLUSION: HBsAg-pulsed DCs can enhance the proliferation and killing functions of CIK cells.
Assuntos
Células Matadoras Induzidas por Citocinas/imunologia , Células Dendríticas/imunologia , Antígenos de Superfície da Hepatite B/imunologia , Linhagem Celular Tumoral , Proliferação de Células , Antígenos de Superfície da Hepatite B/fisiologia , HumanosRESUMO
OBJECTIVE: To analyze the clinical features of severe acute respiratory syndrome (SARS). METHODS: The clinical features of 35 patients with SARS in the past five months were retrospectively studied, and compared with 35 patients with community-acquired pneumonia. Consecutive blood samples from 13 patients with SARS and 10 healthy volunteers were collected. The CD+4 and CD+8 in T cell in peripheral blood were detected by flow cytometry. RESULTS: The most common symptoms included fever (in 100.0 percent of the patients), cough (74.3 percent), headache (45.7 percent), myalgia (45.7 percent) and lymphopenia (20/33). Serial chest radiographs showed progressive multi-infiltration in the lung fields. The CD+4 and CD+8 in T cell in 13 patients with SARS significantly decreased [CD+4: (16.10+/-4.31) percent vs. (38.30+/-8.52) percent, t=8.174,P<0.01; CD+8: (19.90+/-5.40) percent vs. (28.38+/-4.33) percent, t=4.055, P<0.01]. The time of bringing down the fever and the time of absorption of pathological changes in SARS patients were prolonged than those of the pneumonia patients [the time of bringing down the fever (13.92+/-8.35) days vs. (3.86+/-1.42)days, t=16.490,P=0.000;the time of absorption of pathological changes: (11.97+/-4.41) days vs. (9.21+/-4.42) days, t=3.082,P=0.003]. CONCLUSION: SARS is a serious respiratory illness, the most common symptoms are fever, cough, headache and myalgia, other common findings are lymphopenia, the CD+4 and CD+8 in T cell in peripheral blood decrease and multi-infiltrate through out the lung fields.
