Prevalence of multi-drug resistant Pseudomonas aeruginosa isolated from selected residential sewages in Dutsin-Ma, Katsina State, Nigeria.

The global surge in Multidrug resistant (MDR) bacteria is an issue of great concern. Pseudomonas aeruginosa has been implicated in several nosocomial infections, where it has caused grave complications in immunocompromised patients. This is the first study to report the prevalence of MDR P. aeruginosa isolated from residential sewage in Dutsin-Ma, Katsina State, Nigeria. Pseudomonads count, isolation, biochemical characterization and antibiogram were carried out using standard microbiological procedures. This study examined sixty (60) samples from selected residential sewage in the study site collected at different intervals between July and September 2021. A total of 40 (66.7%) P. aeruginosa were isolated from the analyzed sewage samples. The highest (2.84x104) pseudomonad count was recorded from sewage samples collected from Kadangaru. Pseudomonas aeruginosa isolates from this sample site showed the highest (100%) resistance to cephalosporins (cefuroxime) and nitrofurantoin. Similarly, isolates from Miami area also demonstrated the highest (95%) resistance to a cephalosporin (ceftazidime). All (100%) isolates used in this study showed MDR resistance to tested antibiotics. The occurrence of MDR P. aeruginosa from a residential sewage site that may contaminate drinking water sources in the study area is of public health threat to the inhabitants. Surveillance and molecular epidemiology of antibiotics resistant bacteria are urgently needed in the study area.

Prevalence of multi-drug resistant Pseudomonas aeruginosa isolated from selected residential sewages in Dutsin-Ma, Katsina State, Nigeria

The global surge in Multidrug resistant (MDR) bacteria is an issue of great concern. Pseudomonas aeruginosa has been implicated in several nosocomial infections, where it has caused grave complications in immunocompromised patients. This is the first study to report the prevalence of MDR P. aeruginosa isolated from residential sewage in Dutsin-Ma, Katsina State, Nigeria. Pseudomonads count, isolation, biochemical characterization and antibiogram were carried out using standard microbiological procedures. This study examined sixty (60) samples from selected residential sewage in the study site collected at different intervals between July and September 2021. A total of 40 (66.7%) P. aeruginosa were isolated from the analyzed sewage samples. The highest (2.84x104) pseudomonad count was recorded from sewage samples collected from Kadangaru. Pseudomonas aeruginosa isolates from this sample site showed the highest (100%) resistance to cephalosporins (cefuroxime) and nitrofurantoin. Similarly, isolates from Miami area also demonstrated the highest (95%) resistance to a cephalosporin (ceftazidime). All (100%) isolates used in this study showed MDR resistance to tested antibiotics. The occurrence of MDR P. aeruginosa from a residential sewage site that may contaminate drinking water sources in the study area is of public health threat to the inhabitants. Surveillance and molecular epidemiology of antibiotics resistant bacteria are urgently needed in the study area.

Antibiogram studies of Escherichia coli and Salmonella species isolated from diarrheal patients attending Malam Mande General Hospital Dutsin-Ma, Katsina State, Nigeria.

INTRODUCTION: antibiotics resistant bacteria (ARB) is a worldwide problem. Information on ARB associated with diarrheal stool samples from Dutsin-Ma, Katsina State, Nigeria is scare. METHODS: this study examines 41 stool samples of diarrhea patients from a selected hospital in Dutsin-Ma. Questionnaires were used to collect demographic information and used antibiotics. Bacteria isolation and antibiotics susceptibility tests were determined using standard microbiological techniques. Multidrug resistant (MDR) bacteria were selected based on resistant to ≥3 classes of antibiotics. RESULTS: twenty bacteria that include Escherichia coli (n = 15) and Salmonella spp. (n = 5) were isolated. Pediatric age group (0-5 years) showed highest prevalence of 73.3 and 60% respectively. Illiterate patients showed highest (60%) frequency of Salmonella spp. Tetracycline was mostly observed for treating diarrhea among patients; high resistance to amoxicillin (80%), ampicillin (100%) and tetracycline (73.3%) was noticed in E. coli. To each of amoxicillin and ampicillin, 100% resistance was observed among Salmonella spp. Two and one MDR E. coli and Salmonella spp. were identified respectively. CONCLUSION: high occurrence of studied bacteria among infants and aged adults coupled with some displaying MDR characteristics calls for urgent public health attention, hence, comprehensive studies are needed for the determination of molecular epidemiology of these bacteria for public health surveillance.