A Nonbactericidal Anionic Antimicrobial Peptide Provides Prophylactic and Therapeutic Efficacies against Bacterial Infections in Mice by Immunomodulatory-Antithrombotic Duality.

Although bactericidal cationic antimicrobial peptides (AMPs) have been well characterized, less information is available about the antibacterial properties and mechanisms of action of nonbactericidal AMPs, especially nonbactericidal anionic AMPs. Herein, a novel anionic antimicrobial peptide (Gy-CATH) with a net charge of -4 was identified from the skin of the frog Glyphoglossus yunnanensis. Gy-CATH lacks direct antibacterial effects but exhibits significantly preventive and therapeutic capacities in mice that are infected with Staphylococcus aureus, Enterobacteriaceae coli, methicillin-resistant Staphylococcus aureus (MRSA), or carbapenem-resistant E. coli (CREC). In vitro and in vivo investigations proved the regulation of Gy-CATH on neutrophils and macrophages involved in the host immune defense against infection. Moreover, Gy-CATH significantly reduced the extent of pulmonary fibrin deposition and prevented thrombosis in mice, which was attributed to the regulatory role of Gy-CATH in physiological anticoagulants and platelet aggregation. These findings show that Gy-CATH is a potential candidate for the treatment of bacterial infection.

Multi-Spectral Fusion Based Approach for Arbitrarily Oriented Scene Text Detection in Video Images.

Scene text detection from video as well as natural scene images is challenging due to the variations in background, contrast, text type, font type, font size, and so on. Besides, arbitrary orientations of texts with multi-scripts add more complexity to the problem. The proposed approach introduces a new idea of convolving Laplacian with wavelet sub-bands at different levels in the frequency domain for enhancing low resolution text pixels. Then, the results obtained from different sub-bands (spectral) are fused for detecting candidate text pixels. We explore maxima stable extreme regions along with stroke width transform for detecting candidate text regions. Text alignment is done based on the distance between the nearest neighbor clusters of candidate text regions. In addition, the approach presents a new symmetry driven nearest neighbor for restoring full text lines. We conduct experiments on our collected video data as well as several benchmark data sets, such as ICDAR 2011, ICDAR 2013, and MSRA-TD500 to evaluate the proposed method. The proposed approach is compared with the state-of-the-art methods to show its superiority to the existing methods.